Preparation method of quaternary ammonium salinized fluorescent carbon dot and application of quaternary ammonium salinized fluorescent carbon dot in bacterium resisting and gram positive bacterium and gram negative bacterium differentiating

A technology of gram-positive bacteria and fluorescent carbon dots, which is applied in the field of nanomaterials and nanomedicine, can solve the problems of limited antibacterial effect and low net charge density of antibacterial agents, and achieve low cytotoxicity, avoid drug resistance, and good antibacterial effect of nature

Active Publication Date: 2016-06-29
SOUTHEAST UNIV
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Problems solved by technology

However, due to the low net charge density of the free quaternary am

Method used

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  • Preparation method of quaternary ammonium salinized fluorescent carbon dot and application of quaternary ammonium salinized fluorescent carbon dot in bacterium resisting and gram positive bacterium and gram negative bacterium differentiating
  • Preparation method of quaternary ammonium salinized fluorescent carbon dot and application of quaternary ammonium salinized fluorescent carbon dot in bacterium resisting and gram positive bacterium and gram negative bacterium differentiating
  • Preparation method of quaternary ammonium salinized fluorescent carbon dot and application of quaternary ammonium salinized fluorescent carbon dot in bacterium resisting and gram positive bacterium and gram negative bacterium differentiating

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Preparation of aminated carbon dots (AEEA carbon dots) (reaction principle see Figure 1b ), including the following steps:

[0038] Step 1. Add diethylenetriaminopropyltrimethoxysilane (AEEA) to glycerin, stir and mix;

[0039] Step 2. React in a hydrothermal reactor at 260°C for 12 hours to form a carbon dot solution;

[0040] Step 3. Centrifuge to remove the precipitate, and the supernatant is dialyzed in ultrapure water for 2-3 days with a dialysis bag with a molecular weight cut-off of 1000 to obtain a pure carbon dot solution. Wherein, the volume fraction of the divinyltriaminopropyltrimethoxysilane is 9.1%.

Embodiment 2

[0042] The preparation of quaternized ammonium salt antibacterial carbon dot (CD-12) (reaction principle is shown in Fig. 1), comprises the following steps:

[0043] 1) lauryl betaine, NHS and EDC were activated at room temperature for 0.5 hours in a 2-(N-morpholine)ethanesulfonic acid buffer solution at pH=6 at a molar ratio of 1:10 and 1:20, respectively;

[0044] 2) Add the aminated carbon dots in such a way that the material ratio of dodecyl betaine to the raw material diethylenetriaminopropyltrimethoxysilane for preparing the aminated carbon dots is 5:1, and react at room temperature for 4 hours. Obtain quaternized ammonium salt carbon point;

[0045] 3) Use a dialysis bag with a molecular weight cut-off of 1000 to dialyze the quaternized carbon dot solution in ultrapure water for more than 2 days to obtain a pure quaternized carbon dot solution. The particle size characterization results are shown in Figure 2.

Embodiment 3

[0047] The preparation of aminated carbon dots, this group of examples is similar to Example 1, the difference is that the reaction temperatures in step 2 are 240°C, 245°C, 250°C, 255°C, 265°C, 275°C and 280°C.

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Abstract

The invention provides a preparation method of a quaternary ammonium salinized fluorescent carbon dot and application of the quaternary ammonium salinized fluorescent carbon dot in bacterium resisting and gram positive bacterium and gram negative bacterium differentiating. The preparation method of the quaternary ammonium salinized fluorescent carbon dot mainly comprises the two steps that a surface-aminated carbon dot is prepared, and then alkyl betaine is grafted to the surface of the aminated carbon dot to obtain the quaternary ammonium salinized carbon dot. The prepared quaternary ammonium salinized carbon dot has the good water solution dispersibility and the low cytotoxicity and can effectively inhibit and kill gram positive bacteria. In addition, the quaternary ammonium salinized carbon dot has the good fluorescent property and can selectively enable the gram positive bacteria to be imaged to effectively differentiate the gram positive bacteria and gram negative bacteria.

Description

technical field [0001] The invention relates to the fields of nanomaterials and nanomedicine, especially the surface quaternization of water-soluble carbon dots and its application in the field of antibacterial and bacterial selection. Background technique [0002] In recent years, with the development and popularization of antibiotics, some diseases caused by bacterial infections have been suppressed to a large extent. However, due to some complex mechanisms, pathogens can destroy the host's immune system and cause transport obstacles, so that small molecule antibacterial agents such as antibiotics cannot effectively reach the affected area to inhibit the bacteria; at the same time, due to the abuse of antibiotics, bacterial resistance has also caused Therefore, the development of new antibacterial agents is an urgent challenge nowadays. In addition, bacteria can be divided into Gram-positive bacteria and Gram-positive bacteria according to the Gram staining method, and th...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K31/205A61P31/04
CPCA61K31/205A61K49/005A61K49/0071
Inventor 吴富根杨婧婧张晓东
Owner SOUTHEAST UNIV
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