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Preparation method of high-lignin straw decomposition agent

A straw decomposing agent and lignin technology, which is applied in the preparation of organic fertilizers, biochemical equipment and methods, urea compound fertilizers, etc. problems, to achieve the effect of shortening the decomposing time, low cost, and simple preparation steps

Inactive Publication Date: 2016-07-06
CHANGZHOU DAAO NEW MATERIAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: the straw decomposing agent prepared by compounding effective live bacteria and adding carriers is not ideal due to the combination of strains, especially in the selection of lignin-decomposing bacteria, which makes the decomposing effect unsatisfactory. For the problem of unstable use effect, a method is provided that first collects the intestinal juice of termite worker ants, obtains termite intestinal colonies and cultures them, and then inoculates them with the prepared straw-corroding bacteria liquid into the domestication medium, and screens qualified medium colonies, and continue to domesticate them to obtain high lignin straw medium mixed colonies, and finally inoculate them on the mixed medium for cultivation to prepare decomposing agent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] First select termite worker ants, feed them with straw for 15 days, then wash the termite body surface with 65% ethanol solution, wash with sterile water for 3 times, and then use sterilized pointed tweezers to pull out the intestinal tract and tear the intestinal tract Cystic stomach, collect intestinal juice and place it in 0.9% sterilized physiological saline, use a stirring rod to mash the intestinal tissue to prepare a mixed solution of termite intestinal bacteria; weigh 45 One part of washed bean sprouts, 25 parts of sucrose, and 30 parts of agar were stirred and mixed to prepare a medium solid, and then the medium solid was stirred and mixed with deionized water at a solid-to-liquid ratio of 1:5, and sterilized at 120°C for 30 minutes. Prepare the culture medium, then inoculate the mixture of termite intestinal bacteria prepared above into the medium, shake the culture medium at 28°C and 120r / min for 5 days, prepare termite intestinal colonies, and set aside; then...

example 2

[0022]First, select termite worker ants and feed them with straw for 10 days, then wash the termite body surface with 65% ethanol solution, wash twice with sterile water, and then use sterilized pointed tweezers to pull out the intestines and tear the intestines Cystic stomach, collect intestinal juice and place it in 0.9% sterile saline, use a stirring rod to mash the intestinal tissue to prepare a mixed solution of termite intestinal bacteria; weigh 25 One part of washed bean sprouts, 30 parts of sucrose, and 45 parts of agar were stirred and mixed to prepare a medium solid, and then the medium solid was mixed with deionized water at a solid-to-liquid ratio of 1:5, and sterilized at 120°C for 25 minutes. Prepare the culture medium, then inoculate the mixture of termite intestinal bacteria prepared above into the culture medium, shake the shaker culture at 25°C and 120r / min for 3 days, prepare termite intestinal colonies, and set aside; then wash the straw Clean and pulverize...

example 3

[0025] First select termite worker ants, feed them with straw for 12 days, then wash the termite body surface with 65% ethanol solution, wash twice with sterile water, and then use sterilized pointed tweezers to pull out the intestines and tear the intestines Cystic stomach, collect intestinal juice and place it in 0.9% sterilized physiological saline, use a stirring rod to mash the intestinal tissue to prepare a mixed solution of termite intestinal bacteria; weigh 40 parts by weight One part of washed bean sprouts, 30 parts of sucrose, and 30 parts of agar were stirred and mixed to prepare a medium solid, and then the medium solid was mixed with deionized water at a solid-to-liquid ratio of 1:5, and sterilized at 120°C for 27 minutes. Prepare the culture medium, then inoculate the mixture of termite intestinal bacteria prepared above into the culture medium, shake the shaker culture at 26°C and 120r / min for 4 days, prepare termite intestinal colonies, and set aside; then wash ...

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Abstract

The invention relates to a preparation method of a high-lignin straw decomposition agent, belonging to the technical field of straw decomposition agent preparation. The preparation method comprises the following steps: collecting the intestinal juice of worker termite, obtaining the intestinal bacterial colony of termite and culturing; inoculating a domestication medium with the intestinal bacterial colony of termite and the prepared straw decomposition bacterial liquid; screening out the qualified medium bacterial colony and further domesticizing to obtain a lignin straw medium mixed bacterial colony; and inoculating the mixed medium with the mixed bacterial colony and culturing to obtain the decomposition agent. In the invention, the prepared straw decomposition agent is not influenced by the strain formula and contains a large quantity of lignin decomposers, thus the decomposition effect is good, and the application is relatively stable; and moreover, the decomposition time can be greatly shortened, and the straw can be completely decomposed in 6-8 days.

Description

technical field [0001] The invention relates to a preparation method of a high lignin straw decomposing agent, belonging to the technical field of straw decomposing agent preparation. Background technique [0002] The straw decomposing agent can quickly decompose organic waste such as straw, make the organic matter contained in the straw, phosphorus, potassium and other elements become the nutrients needed for plant growth, and produce a large number of beneficial microorganisms, stimulate crop production, increase soil organic matter, and strengthen plant growth. Stress resistance, reduce the use of chemical fertilizers, improve crop quality, and achieve sustainable agricultural development. [0003] At present, the known straw decomposing agent is made by compounding effective live bacteria (bacteria, fungi or actinomycetes) with the ability to decompose straw and adding carriers, which can increase the accumulated temperature of material decomposing to a certain extent an...

Claims

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Application Information

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IPC IPC(8): C05G1/00C05F17/00C12N1/00
CPCC05C9/00C05F17/00C12N1/00C05F11/00C05F11/08Y02W30/40
Inventor 潘艳丽薛培龙高力群
Owner CHANGZHOU DAAO NEW MATERIAL TECH CO LTD
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