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Preparation method of quick-response glucose-sensitive hydrogel

A hydrogel and glucose technology, applied in the field of biomedical polymer materials, can solve the problems of long response time and weak intensity of external stimuli, achieve the effect of enhancing glucose sensitivity and responsiveness, and improving the stability of use

Active Publication Date: 2016-07-06
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But not all amino groups can achieve this effect, there are still problems such as long response time to external stimuli, weak strength, etc., and as a biological material, some other factors such as toxicity, solubility, etc. still need to be considered

Method used

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  • Preparation method of quick-response glucose-sensitive hydrogel
  • Preparation method of quick-response glucose-sensitive hydrogel
  • Preparation method of quick-response glucose-sensitive hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Weigh 0.0960gAAPBA, 0.1560gAM and 0.0156gMBA respectively, add to 1ml 1% chitosan (CS) solution to dissolve. Pipette 1.2ml of DMAEMA and 10μl of TEMED, and mix well. Then add 0.5ml of ammonium persulfate solution APS (40mg / ml) into the solution, after mixing, quickly pipette 1ml of the solution and add it to the reactor, react for about 1h, and pass N through the whole process 2 protection, and finally a film-like gel is obtained. Put the prepared gel film in distilled water at constant temperature for 48 hours, replace the distilled water every 6 hours, then put the gel film in the buffer solution for 48 hours, replace the solution every 6 hours, and dry the purified gel spare.

[0046] Figure 4 is the infrared spectrum of the synthesized CS / P (AAPBA-DMAEMA-AM) hydrogel.

[0047] Polymer at 3436.98cm -1 The absorption peak is the overlapping peak of -NH and -OH stretching vibration peaks, 1740.01cm -1 and 1637.48cm -1 The absorption peaks at are the characteris...

Embodiment 2

[0049] Weigh 0.0960gAAPBA, 0.310gAM and 0.0156gMBA, respectively, and add them into 1ml of 2% polyvinylpyrrolidone (PVP) solution to dissolve. Pipette 0.6ml of DMAA and 10μl of TEMED, and mix well. Then add 0.5ml of ammonium persulfate solution APS (40mg / ml) into the solution, after mixing, quickly pipette 1ml of the solution and add it to the reactor, react for about 1h, and pass N through the whole process 2 Protect, and finally get a film-like gel Put the prepared gel film in distilled water at constant temperature for 48 hours, replace the distilled water every 6 hours, then put the gel film in the buffer solution for 48 hours, replace the solution every 6 hours , and dry the purified gel for later use.

[0050] The infrared spectrum of PVP / P(AAPBA-DMAA-AM) is as follows Figure 5 . The absorption peaks at 3444cm-1 and 1643cm-1 are the stretching vibration absorption peaks of NH and C=O, respectively, indicating that the polymer contains amido and amidophenylboronic aci...

Embodiment 3

[0052] Weigh 0.1920gAAPBA, 0.638gAM and 0.0156gMBA, respectively, and add them into 1ml of 2% PVP solution to dissolve. Pipette 10 μl of TEMED and mix well. Then add 0.5ml of ammonium persulfate solution APS (40mg / ml) into the solution, after mixing, quickly pipette 1ml of the solution and add it to the reactor, react for about 1h, and pass N through the whole process 2 protection, and finally a film-like gel is obtained. Put the prepared gel film in distilled water at constant temperature for 48 hours, replace the distilled water every 6 hours, then put the gel film in the buffer solution for 48 hours, replace the solution every 6 hours, and dry the purified gel spare.

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Abstract

The invention discloses a preparation method of quick-response glucose-sensitive hydrogel which is quick in response to glucose stimulation in a physiological environment. The preparation method particularly includes adding acrylamidophenylboronic acid and acrylic acid amino ester, N-alkyl acrylamide and acrylamide or methacrylamide and N,N'-methylenebisacrylamide into an alkaline macromolecule aqueous solution, and stirring to dissolve completely so as to obtain a mixed solution; adding catalytic reaction accelerators tetramethylethylenediamine into the mixed solution with uniformly mixing; adding initiators accounting for 0.03-0.3% of monomer mass, into the obtained solution, and conducting room temperature reaction to obtain the hydrogel containing a macromolecular copolymer shown as structural formula (I). Alkaline macromolecules in the preparation method are a macromolecule component containing amino or partially containing lactam group and can enhance glucose sensitivity and responsiveness of the hydrogel in the physiological environment by being added, so that stability in use of the hydrogel is improved.

Description

technical field [0001] The invention relates to a glucose-sensitive hydrogel and a preparation method thereof, belonging to the field of biomedical polymer materials. technical background [0002] Diabetes is a chronic disease caused by disordered blood sugar levels in the body. An effective way to treat or control diabetes is to adjust blood sugar levels in the body in time. The glucose sensitive system with continuous and non-invasive detection of blood glucose concentration in the body has broad application prospects in the control and treatment of diabetes. Glucose-sensitive hydrogels as molecular recognition elements have become one of the core issues of detection systems. Glucose-sensitive hydrogels can use glucose oxidase (GOD) and phenylboronic acid group (PBA) as recognition elements. GOD, ConA can cause immune responses in vivo and cause adverse effects in sensor use and placement. The fully synthetic PBA receptor recognition element can overcome the above short...

Claims

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Application Information

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IPC IPC(8): C08F220/56C08F220/58C08F220/34C08F220/54C08F222/38G01N5/02
CPCG01N5/02C08F220/56C08F222/385C08F220/585C08F220/54C08F220/34
Inventor 陈琛李娜
Owner XI AN JIAOTONG UNIV
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