Method of quickly biodegrading lignocellulose source inhibiting substance

A lignocellulose and biodegradation technology is applied in the field of rapid biodegradation of lignocellulose source inhibitors, which can solve the problems affecting the efficiency of lignocellulose biorefinery process, slow rate of biodegradation process, affecting efficiency of biotransformation process, etc. problems, to achieve the effect of reducing the phenomenon of contamination, saving equipment investment, and reducing energy consumption

Inactive Publication Date: 2016-07-06
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the system of microbial solid-state culture to degrade lignocellulose-derived inhibitors, there is a lack of effective mass transfer and mixing, so that there are temperature gradients and dissolved oxygen limitations in the degradation system, resulting in a slow rate of biodegradation, which greatly affects improving the efficiency of the lignocellulosic biorefinery process
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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Example 2

[0020] 1200g dry straw after dust removal and crushing and 600g dilute sulfuric acid solution with 5% mass fraction were added to the pretreatment reactor equipped with a ribbon stirring paddle to carry out high temperature pretreatment reaction. 100 grams of dry straw contains 2.5 grams of sulfuric acid), the stirring speed is 50 rpm, the temperature is raised to 175 oC, and the reaction is performed for 5 minutes. Take out the pretreated material from the reactor, and take out 2000g and add it to a 10L plastic box. Use 20% mass After the concentration of calcium hydroxide solution was adjusted to pH 5.5, the solid seeds of AmorphothecaresinaeZN1 cultured in solid straw were inoculated with a mass ratio of 10% inoculum to start the static biodegradation process in the form of solid-state fermentation. During the process, samples were taken for the detection of inhibitors. After 48 hours of treatment, the degradation rate of furfural in the straw was 92.51%,...

Example Embodiment

[0021] Example 2

[0022] Add 1200g dry straw after dust removal and crushing and 600g dilute sulfuric acid solution with 5% mass fraction into a pretreatment reactor equipped with a ribbon stirring paddle for high temperature pretreatment reaction. The reaction conditions are 175oC, 2.5% dilute sulfuric acid dosage, stirring The rotating speed is 50 rev / min, the temperature rises to 175oC and the reaction is carried out for 5 minutes, the pretreated material is taken out from the reactor, and 900 g is added to the 5L bioreactor with a ribbon stirring paddle, and the stirring speed is turned on at 50 rev / After the pH value was adjusted to 5.5 with a 20% mass concentration of calcium hydroxide solution, 90 g of solid seeds of AmorphothecaresinaeZN1 cultivated in straw were added to the inoculum at a mass ratio of 10%, and the stirring was stopped after mixing. Start the solid state biodegradation process without aeration. During the process, the stirring was turned on before ...

Example Embodiment

[0023] Example 3

[0024]Add 1200g dry straw after dust removal and crushing and 600g dilute sulfuric acid solution with 5% mass fraction into a pretreatment reactor equipped with a ribbon stirring paddle for high temperature pretreatment reaction. The reaction conditions are 175oC, 2.5% dilute sulfuric acid dosage, stirring The rotating speed is 50 rpm, the temperature is raised to 175oC and the reaction is performed for 5 minutes. The pretreated material is taken out from the reactor, and 900 g is added to a 5L bioreactor with a ribbon stirring paddle. The stirring is turned on, and the rotating speed is 50 rpm. After adjusting the pH value to 5.5 with a 20% mass concentration of calcium hydroxide solution per minute, 90 g of solid seeds of AmorphothecaresinaeZN1 cultured in straw were inserted into the inoculum at a mass ratio of 10%, and the stirring was stopped after mixing. , the solid-state biodegradation process was started, and the ventilation rate was maintained at 5...

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Abstract

The invention relates to a method of quickly biodegrading a lignocellulose source inhibiting substance. The method particularly includes the steps of: 1) pre-treating a lignocellulose raw material; 2) quickly biodegrading the lignocellulose raw material in a reactor to remove the compounds which are generated during the pretreatment and have inhibition effects on subsequent enzymolysis and fermentation; and 3) performing the enzymolysis and subsequent processes to the material treated in the step 2). The method reduces treatment period to 6-36 h on the basis of ensuring biodegrading efficiency on the inhibiting substances, so that efficiency and practicability of the process are greatly improved. The biodegrading process is amplified into reactor level and is integrated with a subsequent conversion process in the same reactor, so that the integration degree of a bio-refining process is increased. The method lays the foundation of application of biodegradation of the inhibiting substances in the lignocellulose bio-refining process.

Description

technical field [0001] The invention relates to the fields of biorefinery and bioenergy, in particular to a method for rapid biodegradation of lignocellulose-derived inhibitors. Background technique [0002] The main process of lignocellulose biorefinery includes pretreatment, pretreatment, enzymatic hydrolysis and saccharification, fermentation production and product separation. The pretreatment process refers to a process in which the structure of lignocellulose is destroyed by physical, chemical and physical-chemical methods, which can effectively increase the conversion of cellulose and hemicellulose in lignocellulose raw materials in subsequent bioprocessing. Efficiency is the most important step in the lignocellulose biorefinery process. However, in the pretreatment process, due to the synergistic effect of high temperature and chemical catalysts, cellulose, hemicellulose and lignin in lignocellulosic raw materials will inevitably undergo excessive degradation, result...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P7/16C12P7/18C12P7/64C12P7/56C12P7/48C12P13/14C12P7/46C12P19/02C12P19/04C12P19/14
CPCY02E50/10
Inventor 鲍杰何燕青张建
Owner EAST CHINA UNIV OF SCI & TECH
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