Method of quickly biodegrading lignocellulose source inhibiting substance

Abstract

The invention relates to a method of quickly biodegrading a lignocellulose source inhibiting substance. The method particularly includes the steps of: 1) pre-treating a lignocellulose raw material; 2) quickly biodegrading the lignocellulose raw material in a reactor to remove the compounds which are generated during the pretreatment and have inhibition effects on subsequent enzymolysis and fermentation; and 3) performing the enzymolysis and subsequent processes to the material treated in the step 2). The method reduces treatment period to 6-36 h on the basis of ensuring biodegrading efficiency on the inhibiting substances, so that efficiency and practicability of the process are greatly improved. The biodegrading process is amplified into reactor level and is integrated with a subsequent conversion process in the same reactor, so that the integration degree of a bio-refining process is increased. The method lays the foundation of application of biodegradation of the inhibiting substances in the lignocellulose bio-refining process.

Description

technical field [0001] The invention relates to the fields of biorefinery and bioenergy, in particular to a method for rapid biodegradation of lignocellulose-derived inhibitors. Background technique [0002] The main process of lignocellulose biorefinery includes pretreatment, pretreatment, enzymatic hydrolysis and saccharification, fermentation production and product separation. The pretreatment process refers to a process in which the structure of lignocellulose is destroyed by physical, chemical and physical-chemical methods, which can effectively increase the conversion of cellulose and hemicellulose in lignocellulose raw materials in subsequent bioprocessing. Efficiency is the most important step in the lignocellulose biorefinery process. However, in the pretreatment process, due to the synergistic effect of high temperature and chemical catalysts, cellulose, hemicellulose and lignin in lignocellulosic raw materials will inevitably undergo excessive degradation, result...

AI Technical Summary

Problems solved by technology

However, in the system of microbial solid-state culture to degrade lignocellulose-derived inhibitors, there is a lack of effective mass transfer and mixing, so that there are temperature gradients and dissolved oxygen limitations in the degradation system, resulting in a slow rate of biodegradation, which greatly affects improving the efficiency of the lignocellulosic biorefinery process

For example, in the Chinese patent with publication number CN102191279A, the applicant used the filamentous fungus Amorphothecaresinae ZN1 to degrade the inhibitors produced after pretreatment of lignocellulosic raw materials, and completely removed the three inhibitors with the highest content of furfural, hydroxymethylfurfural and acetic acid. The time is as long as 4 to 7 days, which greatly affects the efficiency of the subsequent biotransformation process