Grifola frondosa mutant strain for producing grifola frondosa mycelia

A technology of Grifola frondosa and bacterial strains, applied in the field of food microbiological technology, can solve the problems of unstable quality and yield, long cycle of Grifola frondosa, low production efficiency, etc.

Inactive Publication Date: 2016-07-13
JIANGSU UNIV
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  • Abstract
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Problems solved by technology

At present, the main method of obtaining Grifola frondosa is artificial cultivation, using fruiting body or spore powder as medicine, but artificial cultivation ...

Method used

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  • Grifola frondosa mutant strain for producing grifola frondosa mycelia
  • Grifola frondosa mutant strain for producing grifola frondosa mycelia
  • Grifola frondosa mutant strain for producing grifola frondosa mycelia

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Embodiment 1

[0053] Grifola frondosa strain adopts Grifola frondosa CCTCCM2015065. The amount of rice bran used is 8.0g / 100mL medium, the amount of bran used is 8.0g / 100mL medium, and KH 2 PO 4 0.36g / 100mL, MgSO 4‘ 7H 2 O0.36g / 100mL, cysteine ​​hydrochloride 50mg / 100mL, sodium copper chlorophyllin 10mg / 100mL, natural pH, add water to the required volume, sterilize at 121°C for 30min, use it as a culture medium for fermentation, pack in shake bottles The sample volume was 40%, the inoculum volume was 10%, and the culture temperature was 28 o C, rotation speed 180r / min, culture time 7d. The following steps (3) to (8) in the technical solution described in the foregoing summary of the invention are implemented. The results were: mycelium dry weight 7.9g / 100mL medium, mycelia polysaccharide 524.6mg / 100mL medium, exopolysaccharide 763.2mg / 100mL medium, lead content 1.72mg / kg, arsenic content 0.71mg / kg, and the mercury content is 0.07mg / kg, both of which are lower than the limit index val...

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Abstract

The invention discloses a grifola frondosa mutant strain for producing grifola frondosa mycelia, and relates to the field of application of food microorganism technologies.The mutant strain has been deposited in the China Center for Type Culture Collection (CCTCC) at Wuhan on January 23, 2015 with the number of CCTCC M 2015065, and can be named as Grifola frondosa CCTCC M 2015065.The strain is used for fermenting liquid rice bran added with cysteine hydrochloride and sodium copper chlorophyllin and a bran complete feed medium.Compared with the original strain Grifola frondosa CCTCC M 2013286, the grifola frondosa mutant strain for producing grifola frondosa mycelia has the features that the production capacity is higher, the yield of pure mycelia is improved, mycelia polysaccharides and polysaccharides in fermentation liquor are increased correspondingly, the quality of the mycelia is higher, and the grifola frondosa mutant strain is suitable for being used for producing raw materials of health food.

Description

technical field [0001] The invention relates to the application field of food microbiology technology, in particular to a new strain of Grifola frondosa strain obtained by mutagenesis that can be used for a new culture medium for liquid fermenting rice bran and bran whole material to produce Grifola frondosa mycelium raw material. Background technique [0002] Medicinal and edible fungi have a long history of use in China and a wide range of users. Modern science reveals that the mycelium, fruiting bodies or spores of commonly used medicinal and edible fungi such as Grifola frondosa, Ganoderma lucidum, Cordyceps sinensis, Hericium erinaceus, etc. can produce such as amino acids, proteins, vitamins, polysaccharides, nucleosides, Various active ingredients such as flavonoids and antibiotics, or enriched with various trace elements such as selenium and zinc, have various functions such as improving human immunity, anti-tumor, enhancing liver function, and anti-oxidation. Becau...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/145C12R2001/645
Inventor 刘伟民周彬王冲之王雪梅李婷婷程宇任晓锋伍娟朱文静张凤霞狄新园
Owner JIANGSU UNIV
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