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Rheidin application and antiviral drugs

A technology of rhubarb dianthrone and antiviral drugs, applied in the field of medicine, can solve problems such as unacceptable, uncertain curative effect, and unsatisfactory curative effect

Inactive Publication Date: 2016-07-20
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The efficacy of antiviral chemical drugs in the treatment of RSV infection is not ideal, and there are potential toxic and side effects; the efficacy of hormones and bronchodilators is uncertain; passive immune drugs IVIG, RSV-IGIV and Palivizumah are expensive to prevent and treat RSV infection, and are difficult to be accepted by the public; gene therapy and vaccine prevention are still in the research phase

Method used

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  • Rheidin application and antiviral drugs
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  • Rheidin application and antiviral drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 In vitro antiviral pharmacodynamics experiment of rhubarb dianthrone

[0035] Dilute Hep-2 cells (human laryngeal carcinoma epithelial cells) in DMEM medium containing 2% fetal bovine serum at 5×10 4 Inoculate a 96-well culture plate at a concentration of 1 / ml, 100 μl per well, place at 37°C, 5% CO 2 Cell monolayers were formed overnight in an incubator. After the supernatant was discarded, the cells were washed once with PBS to remove the residual fetal bovine serum. They were divided into normal group and experimental group. The experimental group was diluted to 100 TCID with DMEM medium without fetal bovine serum. 50 For each strain of virus, 100 μl per well, after incubation at 35°C for 2 hours, the virus solution was discarded. Rhubarb dianthrone was prepared into different concentrations in DMEM medium with 2% fetal bovine serum, and added to the single layer of Hep-2 cells in the normal group and the experimental group respectively. %CO 2 Cultivate ...

experiment example 2

[0041] Experimental example 2: Protective effect of rhubarb dianthrone on mouse death caused by influenza virus H1N1

[0042] The formal experiment of anti-influenza virus efficacy of rhubarb dianthrone in vivo used 18-20g Balb / C mice, and randomly divided them into 6 groups, 10 mice in each group, half male and half female, respectively normal control group, model group, and rhubarb dianthrone Ketone low dose group 50mg / kg / d, rhubarb dianthrone medium dose group 100mg / kg / d, rhubarb dianthrone high dose group 200mg / kg / d and positive drug ribavirin positive control group 100mg / kg / d . After 3 days of adaptive culture, the experiment was started. Except for the normal control group, mice in other groups were lightly anesthetized with ether, and intranasally inoculated with normal saline equivalent to 8LD 50 Influenza virus A / FM1 / 1 / 47 (H1N1) chicken embryo allantoic fluid 50μl / only, the normal control group was inoculated with equal volume of normal saline, each administration g...

experiment example 3

[0047] Experimental Example 3: Protective Effect of Rhubarb Danthrone on Influenza Virus Pneumonia in Mice

[0048] The modeling, grouping, and administration of Balb / c mice were the same as in Experimental Example 2. On the 6th day after the mice were infected with the virus, they were fasted for 8 hours, their body weight was recorded, their eyes were extirpated by bloodletting, they were sterilized with alcohol, and the whole lungs were aseptically removed. The lung tissue was washed twice with saline, blotted dry with filter paper, and the lung weight was recorded. The lung index and lung index inhibition rate were calculated according to the following formula, and the results are shown in Table 4.

[0049]

[0050]

[0051] Table 4 Protective effect of rhubarb dianthrone on viral pneumonia in mice infected with influenza virus

[0052]

[0053] Note: **P<0.01, *P<0.05 compared with model group

[0054] It can be seen from Table 4 that the lung index of the mice...

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Abstract

The invention provides application of rheidin in preparing drugs for treating viral diseases. It is proved through experiments that rheidin has the inhibiting effects on cell lesions induced by an influenza virus, a respiratory syncytial virus, a herpes virus and enterovirus EV71 type, and the survival rate of virus infected cells can be increased; meanwhile, virus replication can be inhibited in a body, the survival rate of virus infected animals is increased, the survival time of the virus infected animals is prolonged, symptoms of diseases caused by virus infection are relieved, and therefore the effects of treating influenza, viral pneumonia, upper respiratory tract infection, skin herpes simplex viruses and hand-foot-mouth diseases and an application value are achieved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the use of rhubarb dianthrone and antiviral drugs. Background technique [0002] Rhubarb is the dry root and rhizome of Rheumpalmatum L., Rheumtanguticum Maxim.ExBalf, or RheumofficinaleBaill. of the Polygonaceae plant. It was first recorded in "Shen Nong's Herbal Classic". Spleen, Stomach, Large Intestine, Liver, and Pericardium Meridians have various functions such as purging and attacking stagnation, clearing heat and purging fire, detoxifying, stopping bleeding, promoting blood circulation and removing stasis. In recent years, a large number of researches on rhubarb have been carried out at home and abroad. Its pharmacological effects have been continuously recognized, and the scope of clinical application has gradually expanded. Coupled with the continuous recognition of traditional Chinese medicine, the clinical use of rhubarb has increased year by year. Rhuba...

Claims

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Application Information

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IPC IPC(8): A61K31/192A61K31/122A61P11/00A61P17/00A61P31/12A61P31/14A61P31/16A61P31/22A61K36/708
CPCA61K31/122A61K31/192A61K36/708
Inventor 萧伟丁玥曹泽彧李娜曹亮丁岗王振中
Owner JIANGSU KANION PHARMA CO LTD
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