Method for refining fish oil from leftovers of freshwater fish
A technology of leftovers and freshwater fish, which is applied in the fields of fat oil/fat refining, fat oil/fat production, fat production, etc. It can solve the problems of low free fatty acid content, pollution, and low bioavailability, and achieve low free fatty acid content, The effect of mild action conditions and rich sources of raw materials
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example 1
[0020] First, take 500g of fresh black fish, slaughter it, take the fat part of the head, viscera and abdomen, clean the blood stains with clean water, put them in a meat grinder and grind them to form a homogenate, and add 5 times the volume of Stir with distilled water, put it into an enzymolysis tank after stirring, set the temperature at 55°C, add compound protease at 1200u / g, adjust the pH value to 7.0 with 10% sodium hydroxide and hydrochloric acid, and carry out the enzyme under stirring After hydrolysis for 1 hour, the enzymolysis solution was deenzyme treated at 100°C for 10 minutes; the compound protease was prepared by using trypsin, papain, neutral protease, pepsin and alkaline protease according to the mass ratio of 1:2:1:2 : 1 mixed; the enzymolysis solution after the above-mentioned deenzyme treatment was cooled to room temperature, centrifuged at a speed of 6000r / min for 15min with a centrifuge to obtain a supernatant, and the supernatant was mixed with petroleu...
example 2
[0023]First, take 750g of fresh black fish, slaughter it, take the head, viscera and abdominal fat part, clean the blood stains with clean water, put them in a meat grinder and grind them to form a homogenate, add 5 times the volume of Stir with distilled water, put it into the enzymolysis tank after stirring, set the temperature at 60°C, add compound protease at 1200u / g, adjust the pH value to 7.0 with 10% sodium hydroxide and hydrochloric acid, and carry out the enzyme under stirring After hydrolysis for 2 hours, the enzymolysis solution was deenzyme-treated for 13 minutes at 103°C; the composite protease was prepared by using trypsin, papain, neutral protease, pepsin and alkaline protease in a mass ratio of 1:2:1:2 : 1 mixed; the enzymolysis solution after the above-mentioned deenzyme treatment was cooled to room temperature, centrifuged at a speed of 7000r / min for 17min with a centrifuge to obtain a supernatant, which was mixed with petroleum ether at a volume ratio of 1:2 ...
example 3
[0026] First, take 1000g of fresh black fish, slaughter it, take the head, viscera and abdominal fat part, clean the blood stains with clean water, put them in a meat grinder and grind them to form a homogenate, add 5 times the volume of Stir with distilled water, put it into the enzymolysis tank after stirring, set the temperature at 65°C, add compound protease at 1200u / g, adjust the pH value to 7.0 with 10% sodium hydroxide and hydrochloric acid, and carry out the enzyme under stirring After hydrolysis for 3 hours, the enzymolysis solution was deenzyme-treated for 15 minutes at 105°C; the composite protease was prepared by using trypsin, papain, neutral protease, pepsin and alkaline protease in a mass ratio of 1:2:1:2 : 1 mixed; the enzymolysis solution after the above-mentioned deenzyme treatment was cooled to room temperature, centrifuged at 8000r / min for 20min with a centrifuge to obtain a supernatant, and the supernatant was mixed with petroleum ether at a volume ratio of...
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