Method for refining fish oil from leftovers of freshwater fish

A technology of leftovers and freshwater fish, which is applied in the fields of fat oil/fat refining, fat oil/fat production, fat production, etc. It can solve the problems of low free fatty acid content, pollution, and low bioavailability, and achieve low free fatty acid content, The effect of mild action conditions and rich sources of raw materials

Inactive Publication Date: 2016-07-20
CHANGZHOU DINGRI ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem mainly solved by the present invention: traditional fish oil is extracted from deep-sea fish, the cost is high, the resources of deep-sea fish that can be used for fish oil processing are limited, and the method of obtaining fish oil by traditional methods is either low in output or polluted. In the process of fish oil, it is easy to introduce impurity ions, which will cause the fish oil to be polluted, which is not conducive to human health and makes its bioavailability low. A method for refining fish oil from freshwater fish leftovers is provided. The invention uses the leftovers of black fish The raw material is enzymolysi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] First, take 500g of fresh black fish, slaughter it, take the fat part of the head, viscera and abdomen, clean the blood stains with clean water, put them in a meat grinder and grind them to form a homogenate, and add 5 times the volume of Stir with distilled water, put it into an enzymolysis tank after stirring, set the temperature at 55°C, add compound protease at 1200u / g, adjust the pH value to 7.0 with 10% sodium hydroxide and hydrochloric acid, and carry out the enzyme under stirring After hydrolysis for 1 hour, the enzymolysis solution was deenzyme treated at 100°C for 10 minutes; the compound protease was prepared by using trypsin, papain, neutral protease, pepsin and alkaline protease according to the mass ratio of 1:2:1:2 : 1 mixed; the enzymolysis solution after the above-mentioned deenzyme treatment was cooled to room temperature, centrifuged at a speed of 6000r / min for 15min with a centrifuge to obtain a supernatant, and the supernatant was mixed with petroleu...

example 2

[0023]First, take 750g of fresh black fish, slaughter it, take the head, viscera and abdominal fat part, clean the blood stains with clean water, put them in a meat grinder and grind them to form a homogenate, add 5 times the volume of Stir with distilled water, put it into the enzymolysis tank after stirring, set the temperature at 60°C, add compound protease at 1200u / g, adjust the pH value to 7.0 with 10% sodium hydroxide and hydrochloric acid, and carry out the enzyme under stirring After hydrolysis for 2 hours, the enzymolysis solution was deenzyme-treated for 13 minutes at 103°C; the composite protease was prepared by using trypsin, papain, neutral protease, pepsin and alkaline protease in a mass ratio of 1:2:1:2 : 1 mixed; the enzymolysis solution after the above-mentioned deenzyme treatment was cooled to room temperature, centrifuged at a speed of 7000r / min for 17min with a centrifuge to obtain a supernatant, which was mixed with petroleum ether at a volume ratio of 1:2 ...

example 3

[0026] First, take 1000g of fresh black fish, slaughter it, take the head, viscera and abdominal fat part, clean the blood stains with clean water, put them in a meat grinder and grind them to form a homogenate, add 5 times the volume of Stir with distilled water, put it into the enzymolysis tank after stirring, set the temperature at 65°C, add compound protease at 1200u / g, adjust the pH value to 7.0 with 10% sodium hydroxide and hydrochloric acid, and carry out the enzyme under stirring After hydrolysis for 3 hours, the enzymolysis solution was deenzyme-treated for 15 minutes at 105°C; the composite protease was prepared by using trypsin, papain, neutral protease, pepsin and alkaline protease in a mass ratio of 1:2:1:2 : 1 mixed; the enzymolysis solution after the above-mentioned deenzyme treatment was cooled to room temperature, centrifuged at 8000r / min for 20min with a centrifuge to obtain a supernatant, and the supernatant was mixed with petroleum ether at a volume ratio of...

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Abstract

The invention discloses a method for refining fish oil from leftovers of freshwater fish, belonging to the field of fish oil preparation. The method comprises the steps of carrying out enzymolysis and extraction on leftovers of black fish as a raw material to obtain crude fish oil, and carrying out degumming, deacidification, decoloration and deodorization on the crude fish oil, so as to obtain refined fish oil. The method for extracting fish oil from the black fish has the beneficial effects that the raw material sources are rich, and the cost is low; furthermore, the fish oil is prepared by virtue of an enzymolysis method, action conditions are mild, the oil extraction rate is high, the color of the fish oil is light, and the content of free fatty acid is low; by carrying out degumming, deacidification, decoloration and deodorization on the crude fish oil to obtain the high-quality fish oil, the value of the fish oil is adequately utilized; and furthermore, the process is simple, and the operation is convenient.

Description

technical field [0001] The invention discloses a method for refining fish oil from leftovers of freshwater fish, belonging to the field of fish oil preparation. Background technique [0002] Fish oil is a general term for all oils in fish, including body oil, liver oil and brain oil. The main fish oil is a kind of oil extracted from fatty fish, rich in omega-3 polyunsaturated fatty acids (DHA and EPA ), has health benefits such as anti-inflammatory and blood lipid regulation. my country is a major producer of freshwater fish in the world. In 2005, the national freshwater fish output was as high as 20.09 million tons. In freshwater fish processing, 50-70% of fish body tissues (fish head, fish viscera, fish abdomen, etc.) become leftovers, which contain a large amount of fish oil. The head, viscera and abdominal muscles of fish contain a large amount of high-quality fish oil, and freshwater fish oil is rich in polyunsaturated fatty acids, especially omega-3 polyunsaturated f...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/10C11B3/04C11B3/06C11B3/10
CPCC11B1/025C11B1/10C11B3/001
Inventor 裴俊王统军
Owner CHANGZHOU DINGRI ENVIRONMENTAL PROTECTION TECH
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