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A kind of Bacillus wdgj11 and its application in improving the quality of tobacco leaves

A technology of bacillus, cgmccno.12111, applied in the direction of bacteria, microbe-based methods, microbes, etc., can solve the problems of flue-cured tobacco quality, reduce plant diseases and insect pests, have good application prospects, and increase plant chlorophyll content.

Inactive Publication Date: 2019-04-02
SICHUAN AGRI UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the quality of flue-cured tobacco is affected to a certain extent because the area contains minerals such as feldspar and mica that plants cannot absorb and utilize when planting flue-cured tobacco in this area.

Method used

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  • A kind of Bacillus wdgj11 and its application in improving the quality of tobacco leaves
  • A kind of Bacillus wdgj11 and its application in improving the quality of tobacco leaves
  • A kind of Bacillus wdgj11 and its application in improving the quality of tobacco leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 contains the bacterial agent of bacillus WDGJ11

[0029] The fungus was isolated from the rhizosphere soil of Panzhihua flue-cured tobacco, and it was found that it had a strong potassium-dissolving effect. Inoculate the bacteria into the rhizosphere of flue-cured tobacco seedlings, which can decompose mineral potassium in the soil, such as feldspar and mica, which cannot be absorbed by flue-cured tobacco to produce water-soluble potassium, which can be absorbed and utilized by flue-cured tobacco to promote the growth of flue-cured tobacco and improve Tobacco quality.

[0030] The strain was identified as Bacillus sp., numbered WDGJ11, and preserved in the General Microbiology Center of China Committee for Culture Collection of Microorganisms (CGMCC for short, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China Institute of Microbiology, Chinese Academy of Sciences, postal code: 100101), the date of deposit is January 27, 2016,...

Embodiment 2

[0032] Indoor test effect of embodiment 2 microorganisms on the growth promotion of flue-cured tobacco

[0033] The bacterium agent described in embodiment 1 is diluted 500 times.

[0034] The natural farming soil was used as the soil matrix for indoor potting experiments. First, the natural farming soil was mixed with flue-cured tobacco organic fertilizer and compound fertilizer evenly, and then put into plastic pots, and the flue-cured tobacco seedlings with uniform growth were transplanted into pots filled with soil. After diluting with Bacillus pumilus (Bacillus pumilus) WDGJ11 bacterial agent, water it around the root system of the transplanted flue-cured tobacco seedlings, so that the microbial concentration is 10 9 ~10 10 Bacterial cells / plants, and no fungal agent was used as the control (CK), and each treatment was repeated three times; the base fertilizer, topdressing and watering of the whole treatment were managed according to the conventional measures of flue-cur...

Embodiment 3

[0038] Example 3 Microorganisms Improve the Outdoor Test Effect of Flue-cured Tobacco Quality

[0039] The outdoor effect test of microbes improving the quality of flue-cured tobacco was carried out in the Panzhihua plain tobacco area; and Yunyan 85 was used as the tested variety, but it is not limited to this variety or this kind of plant.

[0040] With the inoculum containing bacillus WDGJ11 prepared by example 1, the experiment will dilute the inoculum 200 times as root-fixing water irrigation (5 × 10 9 bacteria cells / tobacco strain) as treatment TB, and conventional organic fertilizer without Bacillus WDGJ11 as control CK, each treatment was repeated three times and arranged in random blocks, 40 tobacco seedlings in each plot, and 1 row was designed between plots Separate rows, and design two rows at the edge of the test field as protection rows. The base fertilizer (vermicompost organic fertilizer 50kg / mu, compound fertilizer 50kg / mu), topdressing, watering, weeding and ...

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Abstract

The invention relates to the fields of microorganisms and tobacco planting, and specifically discloses a bacillus and an application thereof. The invention screens and obtains a bacillus WDGJ11 with high-efficiency potassium-decomposing ability, and its preservation number is CGMCC No. 12111. The bacterium agent and bacterium fertilizer prepared by using it can effectively promote plant growth, increase plant chlorophyll content and reduce plant diseases and insect pests. On this basis, the present invention establishes a stable, efficient, and promising method for improving the quality of flue-cured tobacco and improving the quality of tobacco planting soil, that is, applying the bacillus to the plant rhizosphere in the form of bacterial agent or bacterial fertilizer The method for promoting the growth of flue-cured tobacco is mainly applied to the tobacco planting soil, especially the farmland system with unbalanced soil nutrients and continuous cropping obstacles. At the same time, the application of this method can restore the micro-ecological balance of flue-cured tobacco rhizosphere, reduce continuous cropping obstacles, adjust soil nutrient balance, and have a certain effect on the restoration of tobacco planting soil.

Description

technical field [0001] The invention relates to the fields of microorganisms and tobacco planting, in particular to a bacillus WDGJ11 and a method for improving the quality of flue-cured tobacco leaves by using it. Background technique [0002] Tobacco is one of the important economic crops in our country, and it is also an important economic source of national and local fiscal and taxation, which plays a role that cannot be ignored in the local economic development. With the improvement of living standards, people pay more and more attention to the contradiction between smoking and health, which is mainly manifested in that the requirements for cigarettes are not only limited to the taste and aroma, but also the internal quality of tobacco leaves. However, the internal quality, appearance color and luster of tobacco leaves are closely related to the type of fertilizer and the composition of fertilization. [0003] At present, the fertilization of flue-cured tobacco in my c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/07
CPCC05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 余秀梅谢雨歆陈强王昌全李斌杨军伟辜运富赵珂徐开未曾庆宾冯文强
Owner SICHUAN AGRI UNIV