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Method for preparing functional peptide from royal jelly

A royal jelly and functional peptide technology, applied in the field of bioengineering, can solve the problems of no reported preparation method of royal jelly protein peptide, no report of hypoglycemic activity of royal jelly protein peptide, etc., so as to promote agricultural quality and efficiency, increase utilization value, Scientific and reasonable effect

Inactive Publication Date: 2016-07-20
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, among the researches that have been reported, there are studies on royal jelly protein peptides for blood pressure reduction, antioxidant activity, and Aβ inhibitors, but there is no report on royal jelly protein peptides’ hypoglycemic activity, and there is no report on royal jelly protein peptides. The optimal preparation method for multiple functional activities

Method used

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  • Method for preparing functional peptide from royal jelly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, a kind of method utilizing royal jelly to prepare functional peptide, carries out following steps successively:

[0030] Weigh 1.0kg of royal jelly (pH 3.8), add 1.0kg of water and stir evenly, heat up to 37°C, then add 10g of acid protease (50,000 U / g), adjust the pH to 3.5 with 0.1mol / L HCl, and The enzymolysis reaction was incubated (37° C.) for 4 hours with stirring.

[0031] After the enzymatic hydrolysis reaction time is up, the enzymatic hydrolysis reaction product is rapidly heated (heating rate: 10°C / min) to 90°C and kept for 10 minutes, and then cooled (heating rate: 10°C / min) to room temperature to terminate the enzymolysis reaction. Adopt J-6M large-capacity refrigerated centrifuge (BECKMAN company) to centrifuge under the condition of rotating speed 3000r / min and 4 ℃ for 20min, obtain supernatant 1.2kg, this supernatant is the enzymolysis solution containing royal jelly functional peptide.

[0032] Under the working pressure of 0.1-0.2Mpa and...

Embodiment 2

[0034] Embodiment 2, a kind of method utilizing royal jelly to prepare functional peptide, carries out following steps successively:

[0035] Weigh 2.0kg of royal jelly (pH value 3.8) with Example 1, add 2.0kg of water and stir evenly, heat up to 40°C, then add 20g of acid protease (50,000 U / g), and use 0.1moL / L of HCl to adjust the pH The value was 4.0, and the enzymolysis reaction was incubated (40° C.) for 4 hours under stirring conditions.

[0036] Subsequent steps are the same as in Example 1.

[0037] Finally, 158g of royal jelly functional peptide powder was obtained, the peptide content was 92.8%, the color was milky white, the flavor was pure and harmonious, and slightly frankincense. The royal jelly peptide powder was dissolved to a concentration of 1.0 mg / mL, and its DPPH free radical scavenging rate was measured to be 84.2%; at the same concentration of 1.0 mg / mL, the inhibitory activity IC of the active peptide on ACE enzyme was detected by HPLC 50 0.07mg / mL; wh...

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Abstract

The invention discloses a method for preparing functional peptide from royal jelly. The method comprises the following steps: uniformly stirring royal jelly with water, heating the prepared royal jelly to 35-40 DEG C, adding acid proteinase, adjusting the pH value to 3.5-4.0, performing temperature-keeping enzymatic hydrolysis for 3.5-4.5 hours under a stirring condition, wherein the obtained enzymatic hydrolysate contains royal jelly functional peptide; performing ultra-filtration treatment on the enzymatic hydrolysate by using an ultra-filtration membrane at the working pressure of 0.1-0.2MPa and the working temperature of 20-45 DEG C so as to obtain ultra-filtration enzymatic hydrolysate; keeping the temperature of the ultra-filtration enzymatic hydrolysate at 70-80 DEG C so as to prevent microbial spoilage; and performing spray-drying, thereby obtaining royal jelly functional peptide powder.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing functional peptides from royal jelly. Background technique [0002] With the development of society and economy, people's health awareness is becoming stronger and stronger, and they are paying more and more attention to the quality and nutrition of food. Bioactive peptides are multifunctional compounds derived from proteins, which play an important regulatory role in life activities. They are easy to digest and absorb, and have the functions of promoting immunity, hormone regulation, antibacterial, antiviral, antioxidation, lowering blood pressure, lowering blood fat, and lowering blood sugar. It has high food safety and is currently the most popular research topic in the international food industry and a functional factor with great development prospects. At present, functional peptide research has been included in the high-tech fields...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06
Inventor 朱作艺王伟张玉王君虹李雪袁玉伟胡桂仙
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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