Ciprofloxacin medicine composition and application thereof to biological medicine
A technology of ciprofloxacin and its composition, which is applied in the field of pharmaceutical composition and its application in biomedicine, can solve the problems that have not yet been reported on the correlation of ciprofloxacin with diabetic cardiomyopathy, and achieve the effect of improving the effect
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Embodiment 1
[0021] Example 1: Compound (I) Separation Preparation and Structure Confirmation
[0022] Sources of reagents: ethanol, petroleum ether, ethyl acetate, n-butanol, and dichloromethane were of analytical grade, purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Methanol, of analytical grade, were purchased from Jiangsu Hanbang Chemical Reagent Co., Ltd.
[0023] Separation method: (a) Grind the dried rhizome (2kg) of Rhizoma Chuanxiong, extract with 65% ethanol under heat reflux (20L×3 times), combine the extracts, concentrate until there is no alcohol smell (4L), and then use petroleum ether (4L× 3 times), ethyl acetate (4L × 3 times) and water-saturated n-butanol (4L × 3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) step (a) The n-butanol extract was removed with AB-8 macroporous resin, first eluted with 10% ethanol for 12 column volumes, then with 70% ethanol for 15 column volumes, and collected...
Embodiment 2
[0027] Embodiment 2: pharmacological action
[0028] 1. Materials and methods
[0029] 1.1 Animals
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