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Composite probiotic-fermented alfalfa sprout powder preparation and preparation method and application thereof

A technology of compounding probiotics, probiotics, applied in the direction of biochemical equipment and methods, microorganism-based methods, bacteria used in food preparation, etc.

Active Publication Date: 2016-07-27
BEIJING SCITOP BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, alfalfa multi-fermentation is applied to silage, but there is no such compound probiotic fermented alfalfa sprout powder preparation

Method used

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  • Composite probiotic-fermented alfalfa sprout powder preparation and preparation method and application thereof
  • Composite probiotic-fermented alfalfa sprout powder preparation and preparation method and application thereof
  • Composite probiotic-fermented alfalfa sprout powder preparation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] To study the effect of different sterilization methods on alfalfa fermentation medium.

[0042] Solid-state fermentation is different from liquid state, and has the difficulties of slow heat transfer, uneven heat transfer, and incomplete sterilization. To study the killing effect of different sterilization methods on miscellaneous bacteria in alfalfa sprout powder. For specific results, see figure 1 . As the temperature increased, the original miscellaneous bacteria in alfalfa powder gradually decreased, and the sterilization effect was the best when the sterilization condition was 121°C for 15 minutes; in addition, compared with bacteria (mainly Bacillus), fungi had lower heat resistance, 95 The sterilization effect can be achieved at ℃ for 30 minutes. see details figure 1 shown.

Embodiment 2

[0044] Study on the effect of soybean protein on fermented alfalfa sprout powder with compound probiotics.

[0045] The inoculum size and the ratio of each bacterial agent were the same.

[0046] a. Fermentation medium: 1000g of alfalfa sprout powder, 0g of sucrose, 0g of soybean protein powder, and the ratio of material to water is 1:1. The number of viable lactic acid bacteria in fermented alfalfa powder was 4.93×10 9 CFU / g.

[0047] b. Fermentation medium: 1000g of alfalfa sprout powder, 0g of sucrose, 10g of soybean protein powder, and the ratio of material to water is 1:1. The number of live lactic acid bacteria in fermented alfalfa powder was 7.09×10 9 CFU / g.

[0048] The test results show that the protein-treated alfalfa sprout powder is beneficial to the growth of lactic acid bacteria. After protein treatment, the number of live lactic acid bacteria in fermented alfalfa was 7.09 billion CFU / g, which was 43.9% higher than that of untreated alfalfa. see details f...

Embodiment 3

[0050] Study on the effect of sucrose on probiotic fermented alfalfa sprout powder.

[0051] The inoculum size and the ratio of each bacterial agent were the same.

[0052] a. Alfalfa sprouts powder 1000g, sucrose 0g, soybean protein powder 10g, material to water ratio 1:1. The number of viable lactic acid bacteria in fermented alfalfa powder was 6.75×10 9 CFU / g.

[0053] b. 1000g of alfalfa sprout powder, 10g of sucrose, 10g of soybean protein powder, and the ratio of material to water is 1:1. The number of viable lactic acid bacteria in fermented alfalfa powder was 1.015×10 10 CFU / g.

[0054] c. 1000g of alfalfa sprout powder, 20g of sucrose, 10g of soybean protein powder, and the ratio of material to water is 1:1. The number of viable lactic acid bacteria in fermented alfalfa powder was 1.102×10 10 CFU / g.

[0055] The test results showed that the alfalfa sprout powder added with sucrose was beneficial to the growth of lactic acid bacteria. After the addition, the nu...

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Abstract

The invention provides a composite probiotic-fermented alfalfa sprout powder preparation and a preparation method and application thereof. Tactobacillus plantarum SCI-01, lactobacillus plantarum P-8, bifidobacterium animalis lactis V9 and lactobacillus casei Zhang are taken as fermentation bacteria, and the preservation numbers of the tactobacillus plantarum SCI-01, the lactobacillus plantarum P-8, the bifidobacterium animalis lactis V9 and the lactobacillus casei Zhang are CGMCC No.11930, CGMCC No.6312, CGMCC No.5470 and CGMCC No.5469; a fermentation culture medium is prepared from, by weight, 970-1,000 parts of alfalfa sprout powder, 8-12 parts of sucrose and 8-12 parts of soybean protein powder, and the material-water ratio is 1:1; the initial pH value is 6.0, the total inoculation quantity is 1*10<7> CFU / g, fermentation is performed at 37 DEG C, and fermentation stops when the pH value is 5.0; a fermentation product is subjected to vacuum freeze drying to obtain the probiotic alfalfa raw powder preparation, the live bacterium number of probiotics reaches 1.0*10<10> CFU / g or above, and the probiotic alfalfa raw powder preparation can be prepared into tablets, powder or capsules after being directly mixed with xylitol.

Description

technical field [0001] The present invention relates to a kind of compound probiotic fermented preparation, relate in particular to a kind of alfalfa sprout powder preparation and its The preparation method and application belong to the technical field of probiotic fermentation preparations. Background technique [0002] Probiotics are a class of microorganisms that have beneficial effects on the host by improving the gut microbiome. It can inhibit the growth of pathogenic bacteria by participating in the balance of intestinal microorganisms, and avoid drug resistance and superinfection caused by long-term use of antibiotics. Therefore, probiotic products have attracted people's attention in recent years. At present, although probiotic products on the market are extremely rich, the developed products are mainly fermented dairy products, which contain high fat and cholesterol, and may also cause lactose intolerance. Therefore, non-dairy probiotic products have become a deve...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04A23L33/135C12R1/25C12R1/245C12R1/01
CPCC12N1/20C12P1/04C12N2500/76C12R2001/245C12N1/205C12R2001/25C12R2001/01A23V2400/125A23V2400/515A23V2400/169
Inventor 刘晓军张建军
Owner BEIJING SCITOP BIO TECH CO LTD
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