Bacillus licheniformis strain screening method

Abstract

Disclosed is a Bacillus licheniformis strain screening method. By using the dibbling method, screening of 200 strains can be completed by only screening one time for about 16 days. The screening process is high in objective orientation, massive screening is performed at the early stage of dibbling, and workload of screening with a shake bottle at the late stage is reduced; compared with conventional screening methods, workload is reduced by 3 / 4, period is shortened by 3 / 4, and efficiency of the method is four times of the conventional screening methods; screening is low in workload, high in objectiveness, simple to operate and few in error, and a series of problems caused by lumping after single colony culturing are avoided.

Description

technical field [0001] The invention relates to the technical field of microbial screening methods. Background technique [0002] As an agricultural microorganism, Bacillus licheniformis has a strong broad-spectrum bactericidal effect. It is used to make agricultural microbial agents in production. It is necessary to screen out strains with strong bactericidal activity and a high number of viable bacteria. Screening the strains that are really needed is like looking for a needle in a haystack, and the workload is huge. [0003] At present, the specific methods used in the screening process of strains include natural screening, paper culture chromogenic method, color change circle method, transparent circle method, growth circle method and inhibition zone method, etc., and these methods have different defects. Natural screening has a large workload and low purpose, and the operation of the disc culture chromogenic method is complicated. Other ring methods cannot completely s...

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Problems solved by technology

[0003] At present, the specific methods used in the screening process of strains include natural screening, paper culture chromogenic method, color change circle method, transparent circle method, growth circle method and inhibition zone method, etc., and these methods have different defects. Natural screening has a large workload and low purpose, and the operation of the disc culture chromogenic method is complicated. Other ring methods cannot completely spread the single colony evenly during the dilution coating process, which will lead to uneven growth of the ring, and produce rings during the measurement process. large error

The method of cultivating single colonies first and then agglomerating in the zone of inhibition method will cause a series of problems. For example, the use of a uniform punch will lead to contamination between single colonies, and the agar block cannot be taken out from the punch, which will eventually lead to operation failed

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