Method for preparing solid-liquid asynchronous citric acid acidification glycolysis feed

A technology of liquid citric acid and citric acid fermentation liquid, which is applied in the fields of enzyme preparations, microorganisms, and feed, and can solve the problems of not involving adding Chinese herbal medicine and vitamins, expensive raw materials, difficult to use fiber and waste residue and waste liquid, etc.

Inactive Publication Date: 2016-08-31
FLSUGARPEPTIDE BIOLOGY ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] 3. The raw materials of the two patent mediums are all starch-containing products, it is difficult to use fibers and waste residues, and the raw ma

Method used

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  • Method for preparing solid-liquid asynchronous citric acid acidification glycolysis feed
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  • Method for preparing solid-liquid asynchronous citric acid acidification glycolysis feed

Examples

Experimental program
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Effect test

Embodiment 1

[0141] A kind of preparation of liquid first and then solid asynchronous citric acid acidification fermented feed comprises the following steps:

[0142] Crush 80kg of corn and 10kg of broken rice, add 300kg of water to adjust the slurry, add 0.08-0.12kg of liquefaction enzyme and glucoamylase for enzymatic hydrolysis, and obtain 376-378kg of saccharified liquid with slag and 18kg of wet base of sugar slag. In 376-378kg of residue-free saccharification liquid, sterilize and cool at high temperature, insert 10kg of citric acid-producing Aspergillus niger spores, and at a temperature of 26-28°C and a natural pH for initial fermentation, introduce oxygen to carry out organic fermentation. Oxygen fermentation was carried out for 12 hours to obtain 374-376kg citric acid fermentation liquid.

[0143] Mix 80kg of corn, 80kg of rice, 20kg of molasses, 80kg of bran, 18kg of wet base of sugar slag, 2kg of stone powder, and 1.8kg of ammonium nitrate, sterilize at high temperature, and co...

Embodiment 2

[0147] The preparation of a kind of asynchronous citric acid acidification fermented feed after solidification first, comprises the following steps:

[0148] Mix 120kg of clean water, 150kg of corn, 50kg of rice, 30kg of molasses, 50kg of bran, 1kg of stone powder, and 1.2kg of ammonium nitrate, sterilize at high temperature, and cool to obtain a solid-state fermentation medium. 18kg of Aspergillus niger strains of citric acid, under the conditions of 22-28°C, natural fermentation pH and continuous supplementation of 20kg of sterile water, oxygen was introduced, aerobic fermentation was carried out for 16 hours, and 390-395kg of citric acid solid-state fermentation culture was obtained Moisture basis.

[0149] After crushing 90kg of broken rice and 10kg of corn, add 300kg of water to adjust the slurry, add 0.08-0.12kg of liquefying enzyme and glucoamylase to enzymatically hydrolyze when the temperature rises to 95°C, and obtain 400kg of slag-containing sugar liquid, sterilize ...

Embodiment 3

[0154] A kind of preparation of liquid first and then solid asynchronous citric acid acidification fermented feed comprises the following steps:

[0155] Crush 100kg of broken rice, add 400kg of water to make slurry, add 0.10-0.12kg of liquefaction enzyme and glucoamylase to enzymatically hydrolyze, and obtain 500kg of saccharified liquid with slag. Through solid-liquid separation, 18kg of sugar residue wet base and 482kg of residue-free saccharification liquid were obtained.

[0156] In 482kg of residue-free saccharification liquid, sterilize and cool at high temperature, insert 10kg of citric acid-producing Aspergillus niger spores, and carry out aerobic fermentation by introducing oxygen at a temperature of 26-28°C and a natural initial fermentation pH In 15 hours, 485kg of citric acid fermentation broth was obtained.

[0157] Mix 100kg of corn, 60kg of rice, 20kg of molasses, 80kg of bran, 18kg of wet base of sugar residue, 2kg of stone powder, and 1.8kg of ammonium nitra...

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Abstract

The invention relates to a method for preparing solid-liquid asynchronous citric acid acidification glycolysis feed. The method includes inoculating citric acid producing bacteria in liquid media at first, fermenting the citric acid producing bacteria in the liquid media for a plurality of hours, then inoculating the citric acid producing bacteria in solid media, continuing to ferment the citric acid producing bacteria in the solid media and producing citric acid glycolysis feed by means of asynchronous fermentation; alternately, inoculating the citric acid producing bacteria in the solid media at first, fermenting the citric acid producing bacteria in the solid media for a plurality of hours, then inoculating the citric acid producing bacteria in the liquid media, continuing to ferment the citric acid producing bacteria in the liquid media and producing the citric acid glycolysis feed by means of asynchronous fermentation. The citric acid glycolysis feed is mixed with one or more types of feeding inorganic acid, organic acid salt, carbohydrate, grease, Chinese herbal medicines and vitamins to obtain mixtures, and the mixtures are dried to obtain the solid-liquid asynchronous citric acid acidification glycolysis feed. The method has the advantages that the solid-liquid asynchronous citric acid acidification glycolysis feed prepared by the aid of the method contains nutrients such as citric acid, protein and crude fibers, is anti-diarrhea and is good in palatability and easy to digest, and the like.

Description

technical field [0001] The invention belongs to the technical fields of feed, enzyme preparations and microorganisms, and in particular relates to a preparation method of solid-liquid asynchronous citric acid acidification fermented feed. Background technique [0002] In recent years, food safety and environmental pollution have been widely concerned by governments at all levels in various countries, which are related to the health and life safety of the people. The safety of animal products requires that the feed has no drug residues, and the environmental protection requires that the feed has low pollution. In the past, we talked about "effective and safe" feed, but now the Ministry of Agriculture has repeatedly emphasized "safe, effective and low pollution". As we all know, since the advent of antibiotics, their use in livestock and poultry to prevent diseases and stimulate growth has indeed received good results. However, with the passage of time, the defects of antibio...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/18A23K10/14A23K10/30A23K10/35A23K10/33A23K10/37A23K20/163A23K20/147A23K10/38A23K10/20A23K10/28A23K20/20A23K20/174A23K20/22A23K20/24A23K20/26A23K20/158
CPCY02P60/87
Inventor 程勐万里刘萱仪孟龙薛芹程茂基
Owner FLSUGARPEPTIDE BIOLOGY ENG
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