Method for extracting pectin from waste sisal residues

A technology of sisal slag and pectin, which is applied in the field of plant component extraction, can solve the problems of low molecular weight of pectin and affect later application, and achieves the effects of light color, low consumption and low degree of esterification

Active Publication Date: 2016-08-31
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

The patent document "extraction method of sisal pectin" (publication number: CN105254778A) discloses a method for extracting pectin from sisal residue by fermentation; the patent document "extraction method of sisal pectin" (published No.: CN103834710A) discloses a method for extracting pectin from sisal residue by enzymatic method, specifically adopts cellulase and pectinase enzymatic hydrolysis extraction, compared with traditional acid method for extracting pectin by enzymatic method, the conditions Mild and pollution-free, using this patented method, the pectin extraction rate is about 10%, and due to the degradation of pectinase, the molecular weight of the extracted pectin is low, which affects the later application

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  • Method for extracting pectin from waste sisal residues
  • Method for extracting pectin from waste sisal residues
  • Method for extracting pectin from waste sisal residues

Examples

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Embodiment 1

[0034] The sisal slag after extracting the fibers was washed with clean water for several times, removed impurities, and dried by blasting at 60 ° C for 10 hours. 2.0mm and 0.5mm sieves, sealed in a sample bag, and stored in a dry place at room temperature; the sisal slag was placed in hot water at 85°C, mixed and stirred, let stand for 20 minutes, rinsed twice with clean water, and drummed at 60°C. Air-dried for 6 hours; take 100 g of dried sisal slag powder, place it in a Soxhlet extractor, use toluene-ethanol (2:1, V / V) as a solvent, extract at 50°C for 4 hours for degreasing, and then take out the sisal slag , at 60°C for 16h by blast drying; add 500mL 0.1mol / L HAc-NaAc buffer solution (pH4. 0), after stirring evenly, add 40 μl of cellulose composite enzyme Celluclast 1.5L, shake and extract in a shaking water bath at 45°C for 18h, then heat the extract at 100°C, continuously boil for 5min to inactivate the enzyme, and then heat it at 4000rpmin. Centrifuge for 8 min in a ...

Embodiment 2

[0037]The sisal slag after extracting the fibers was washed with clean water for several times, removed impurities, and dried by blasting at 60 ° C for 10 hours. 2.0mm and 0.5mm sieves, sealed in a sample bag, and stored in a dry place at room temperature; the sisal slag was placed in hot water at 85°C, mixed and stirred, let stand for 20 minutes, rinsed twice with clean water, and drummed at 60°C. Air-dried for 6 hours; take 100 g of dried sisal slag powder, place it in a Soxhlet extractor, use toluene-ethanol (2:1, V / V) as a solvent, extract at 50°C for 4 hours for degreasing, and then take out the sisal slag , at 60°C for 16h by blast drying; add 800mL 0.1mol / L HAc-NaAc buffer solution (pH4. 0), after stirring evenly, add 85μl of Celluclast 1.5L, shake and extract in a shaking water bath at 45°C for 18h, then heat the extract at 100°C, boil continuously for 5min to inactivate the enzyme, and then heat the extract at 4000rpmin. Centrifuge for 8 min in a centrifuge to obtain...

Embodiment 3

[0039] The sisal slag after extracting the fibers was washed with clean water for several times, removed impurities, and dried by blasting at 60 ° C for 10 hours. 2.0mm and 0.5mm sieves, sealed in a sample bag, and stored in a dry place at room temperature; the sisal slag was placed in hot water at 85°C, mixed and stirred, let stand for 20 minutes, rinsed twice with clean water, and drummed at 60°C. Air-dried for 6 hours; take 100 g of dried sisal slag powder, place it in a Soxhlet extractor, use toluene-ethanol (2:1, V / V) as a solvent, extract at 50°C for 4 hours for degreasing, and then take out the sisal slag , at 60°C for 16h by blast drying; add 500mL 0.1mol / L HAc-NaAc buffer solution (pH4. 0), after stirring evenly, add 40 μl of cellulose composite enzyme Celluclast 1.5L, shake and extract in a shaking water bath at 45°C for 18h, then heat the extract at 100°C, continuously boil for 5min to inactivate the enzyme, and then heat it at 4000rpmin. Centrifuge in a centrifuge...

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Abstract

The invention belongs to the technical field of plant component extraction, and discloses a method for extracting pectin from waste sisal residues. The method includes the steps of smashing sisal residues, killing enzyme with hot water, conducting extracting through a cellulose compound enzyme solution after degreasing to obtain a sisal residue pectin solution extracted through an enzyme method and solid residues, adding solid residues to an ammonium oxalate solution, conducting ultrasonic extracting to obtain a sisal residue pectin solution extracted through an ultrasonic method, combining and concentrating the sisal residue pectin solution, adding absolute ethyl alcohol for sedimentation, and making sediment sequentially subjected to decoloring through an acid alcohol solution and washing through an ethyl alcohol water solution and vacuum drying to obtain sisal residue pectin. The enzyme method and the ultrasonic extracting method are put forward first so that sisal residue pectin can be extracted; compared with an acid extracting method, an oxalic acid and ammonium oxalate extracting method, a fermentation method and the like in the prior art, the pectin extracting rate is increased to be 30-40% from 10-15%.

Description

technical field [0001] The invention belongs to the technical field of plant component extraction, in particular to a method for extracting pectin from discarded sisal slag. Background technique [0002] More and more countries, especially developed countries, have included the transformation and utilization of renewable resources such as agricultural and forestry wastes as an important strategy for sustainable social and economic development. The effective use of agricultural and forestry waste biomass has become the focus of sustainable human development today. one. my country is one of the major sisal producing countries. my country ranks ninth in the world in terms of sisal harvested area, third in the world in sisal fiber production, and first in average unit yield. my country's sisal cultivation is mainly distributed in subtropical regions such as Guangdong, Guangxi, Hainan, and Fujian. Due to the impact of rising labor costs, the total area of ​​sisal cultivation in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/06
CPCC08B37/0003C08B37/0048
Inventor 王兆梅黄舒晴胡蝶
Owner SOUTH CHINA UNIV OF TECH
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