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Electrochemical detection method for bakuchiol and ER-GO/GCE modified electrode special for method

A technology of bakuchiol and modified electrodes, which is applied in the direction of material electrochemical variables, measuring devices, scientific instruments, etc., can solve the problems that there are no ER-GO modified electrodes, etc., and achieve good electrocatalytic activity, high sensitivity, and reproducible Good performance and stability

Inactive Publication Date: 2016-08-31
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the detection of bakuchiol content in psoralen with ER-GO modified electrodes

Method used

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  • Electrochemical detection method for bakuchiol and ER-GO/GCE modified electrode special for method
  • Electrochemical detection method for bakuchiol and ER-GO/GCE modified electrode special for method
  • Electrochemical detection method for bakuchiol and ER-GO/GCE modified electrode special for method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Preparation of ER-GO / GCE Modified Electrode

[0021] 1. Electrode pretreatment

[0022] Before modifying the electrode, the bare glassy carbon electrode (GCE, Φ = 3 mm) was polished on the suede containing 0.05 μm alumina slurry until a smooth mirror surface appeared, and then ultrasonicated with deionized water, absolute ethanol and deionized water successively. Rinse the electrodes for 5 minutes. The platinum wire electrode should be burnt with an alcohol lamp to remove the sundries on the surface, then rinsed with ethanol and acetone in turn, and dried naturally before use.

[0023] 2. Preparation of modified electrodes

[0024] Weigh a certain amount of GO, add different amounts of deionized water, add 0.1moL L -1 LiClO 4 , stirring constantly, and mixing evenly to prepare GO dispersions with different concentrations, the concentrations were 0.1mg mL -1 , 0.3mg mL -1 , 0.5mg mL -1 , 1.0mg mL -1 , 1.5mg mL -1 and 2.0mg mL -1 . Using one-step elec...

Embodiment 2

[0030] Example 2 Detection of Bakuchiol

[0031] The detection method used in this study is differential pulse voltammetry. Before testing, pipette 5mL of PBS (0.1M) solution with nitrogen gas for 10min to deoxygenate, then continuously add a certain concentration of test substances into the closed electrochemical reaction cell with a pipette gun or a micro-injector. Experimental parameters of differential pulse voltammetry: potential gradient: 0.004V; amplitude: 0.05V; pulse width: 0.05s; pulse interval: 0.2s.

[0032] The DPV method has very high sensitivity in trace detection and analysis, so the DPV method was selected to detect the content of bakuchiol. Figure 4 (A) is the DPV response curve of different concentrations of bakuchiol in ER-GO / GCE under optimal conditions, and the modified electrodes were enriched in different concentrations of bakuchiol for 120 s before detection. As shown in Figure 4(B), the peak current is proportional to the concentration of bakuchiol...

Embodiment 3

[0035] Example 3 Detection of bakuchiol in traditional Chinese medicine psoralen from different origins

[0036] Due to the good sensitivity, stability and selectivity of the prepared ER-GO / GCE, this electrochemical sensor can be used for actual sample analysis. Wash, dry, weigh 0.25g and grind the Chinese herbal medicine psoralen from different origins (Zhejiang, Sichuan, Yunnan, Anhui, Jiangxi) purchased on the market, put it in a 50mL volumetric flask, add 30mL methanol, and ultrasonicate for 1h , cooled to constant volume at room temperature. Experiments were performed by standard addition. The experimental results are shown in Table 3, and the relative standard deviation is less than 5%, which shows that the prepared sensor can still be used for the analysis of bakuchiol content in the Chinese herbal medicine Qinpi without the sample purification process, and the results show that this method is useful for detecting The bakuchiol is accurate, appropriate and effective. ...

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Abstract

The invention provides an electrochemical detection method for bakuchiol, and particularly relates to a method for determining the content of bakuchiol by detecting and analyzing test liquid used for preparing a traditional Chinese medicinal material preparation or a Chinese patent medicine preparation by adopting an ER-GO / GCE modified electrode as a working electrode. A result shows that the ER-GO / GCE modified electrode has the good electrocatalytic activity on bakuchiol, is wide in linear range, high in current response speed, low in detection limit, high in sensitivity and interference resisting capacity and good in reproducibility and stability and is successfully applied to detection on the content of bakuchiol contained in fructus psoraleae from different production areas; a novel method for quickly and sensitively determining the content of bakuchiol contained in fructus psoraleae is successfully established.

Description

technical field [0001] The invention relates to a detection method for active components of traditional Chinese medicines, in particular to an electrochemical detection method for bakuchiol and a special ER-GO / GCE modified electrode. technical background [0002] Psoralen is the dried and mature fruit of the leguminous plant Psoralea corylifolia L., which was introduced to China during the Tang and Song dynasties and has a long history of use. The records of relieving asthma, warming the spleen and relieving diarrhea are often used by folks under the aliases such as Po Gu Zhi, Po Gu Zhi, Hu Jiu Zi, Hei Gu Zi, etc. It is mainly distributed in India, Myanmar, Sri Lanka, etc. abroad, and mainly distributed in Shanxi, Shaanxi, Anhui, Zhejiang, Jiangxi, Henan, Hubei, Guangdong, Sichuan, Guizhou, Yunnan and other regions in China. It has the effects of warming the spleen and stopping diarrhea, tonifying the kidney and strengthening yang, consolidating essence and reducing urinati...

Claims

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Application Information

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IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 励娜姚媛媛张小梅陈一龙梁旭明杨大坚
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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