Method for diagnosing plant gray mold by analyzing metabolome of infected plant

A technology of metabolomics and plant ash, which is applied in the fields of microbiology and instrumental analysis, metabolomics, and botany to achieve the effects of convenient operation, accurate and reliable methods, and high discrimination accuracy

Active Publication Date: 2016-09-07
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the application of metabolomics technology in the diagnosis of Botrytis cinerea, and the metabolomic detection method and differential diagnosis method of pathogen-infected plants are yet to be determined by research.

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  • Method for diagnosing plant gray mold by analyzing metabolome of infected plant
  • Method for diagnosing plant gray mold by analyzing metabolome of infected plant
  • Method for diagnosing plant gray mold by analyzing metabolome of infected plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Based on GC-MS analysis of metabolome of strawberry plants, diagnosis and analysis of Botrytis cinerea in asymptomatic samples

[0052] 1. Pretreatment of strawberry plant metabolome samples based on GC-MS analysis

[0053] 1) Inoculation of Botrytis cinerea

[0054] Before inoculation, wash off the dust on the leaf surface with water, and disinfect the leaf surface with 75% ethanol. Adopt the inoculation mode of harmless spray, with 25ml spore suspension (concentration is 10 5 per ml) evenly sprayed to the aboveground parts of strawberry plants. After inoculation, put the flowerpot into the water tray and seal it with plastic film. Strawberry plants inoculated with Botrytis cinerea were treated in darkness at 20°C for 12 hours, and then placed under normal light conditions for cultivation. The control treatment was sprayed with clear water, and the other treatments were the same;

[0055] 2) The collection method is as follows: 2d, 5d, and 7d after inocu...

Embodiment 2

[0081] Example 2 Based on GC-MS analysis of botrytis cinerea infecting strawberry plant metabolome for diagnosis and analysis of Botrytis cinerea

[0082] 1. Pretreatment of metabolome samples of strawberry plants infected by Botrytis cinerea and Strawberry anthracnose based on GC-MS analysis

[0083] 1) Inoculation of Botrytis cinerea and strawberry anthracnose

[0084] Before inoculation, wash off the dust on the leaf surface with water, and disinfect the leaf surface with 75% ethanol. Adopt the inoculation mode of harmless spray, with 25ml spore suspension (concentration is 10 5 per ml) evenly sprayed to the aboveground parts of strawberry plants. After inoculation, put the flowerpot into the water tray and seal it with plastic film. Strawberry plants inoculated with Botrytis cinerea were treated in the dark at 20°C for 12 hours, and then placed under normal light conditions for cultivation. Strawberry plants inoculated with B. fragariae were treated in darkness at 26°C...

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Abstract

The invention provides a method for diagnosing plant gray mold by analyzing metabolome of an infected plant. The method disclosed in the invention diagnoses plant gray mold based on gas chromatographic-mass spectrometric (GC-MS) analysis and partial least squares discriminant analysis (PLS-DA) of the metabolome of the infected plant. The method comprises the following steps: collection of a sample; inactivation with liquid nitrogen; low temperature preservation; extraction of the metabolome; drying; silylation derivatization; GC-MS detection; establishment and detection of a PLS-DA model; etc. With the method provided by the invention, good-reproductivity results of the metabolome of plants infected by pathogens can be obtained; the established PLS-DA model can accurately identify grey mold infected samples without explicit symptoms and precisely identify samples infected by Botrytis cinerea in samples infected by other pathogens and healthy samples. The method is sensitive and accurate, provides an effective method for early diagnosis of gray mold of transplanted seedlings and colonized plants and facilitates early prevention and treatment of field gray mold.

Description

technical field [0001] The invention provides a method for diagnosing botrytis cinerea by analyzing the metabolome of susceptible plants, and relates to the technical fields of metabolomics, botany, microbiology and instrumental analysis. Background technique [0002] Botrytis cinerea is one of the important diseases in the production of vegetables and flowers. It often causes a large number of rotten fruits and vegetables after harvest and during storage and transportation, causing serious economic losses. The pathogen Botrytis cinerea has a wide range of hosts and can infect more than 200 species of plants including Solanaceae, Cucurbitaceae, Rosaceae, Fabaceae, and Grapeaceae. After Botrytis cinerea infects plants such as strawberries, it usually enters a incubation period for a period of time. The symptoms on the leaves are not obvious and can spread with the transplanting of seedlings. This brings troubles to field disease diagnosis and serious consequences for field pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/025
Inventor 刘鹏飞常旭念代探胡志宏耿媛刘子淇宋维珮刘西莉
Owner CHINA AGRI UNIV
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