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Preparation method of three chemical reference substances chebulinic acid, chebuligic acid and ellagic acid in terminalia chebula medicine

A technology of chelic acid and chelic acid is applied in the field of preparation of chemical reference substances, which can solve problems such as restricting research and not being able to fully reflect the quality of myrobalan, achieving the effects of simple operation, easy automatic control and low production cost.

Inactive Publication Date: 2016-09-21
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the chemical composition currently used for the quality control of Myrobalan medicinal materials and its products is gallic acid, which is not enough to fully reflect the quality of Myrobalan
Therefore, the establishment of quality control methods for various chemical components is of great significance for the comprehensive development and utilization of Myrobalan medicinal materials, and the lack of polyphenolic chemical reference substances seriously restricts research in this area
In particular, three chemical reference substances of chebulic acid, chebulic acid and ellagic acid were simultaneously obtained with high purity, but no relevant reports have been seen.

Method used

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  • Preparation method of three chemical reference substances chebulinic acid, chebuligic acid and ellagic acid in terminalia chebula medicine
  • Preparation method of three chemical reference substances chebulinic acid, chebuligic acid and ellagic acid in terminalia chebula medicine
  • Preparation method of three chemical reference substances chebulinic acid, chebuligic acid and ellagic acid in terminalia chebula medicine

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Effect test

Embodiment 1

[0030] The preparation method of three kinds of chemical reference substances of chebulic acid, chebulic acid and ellagic acid in the medicinal material of Myrobalan chebulae comprises the following steps:

[0031] (1) Extraction: After crushing the dried fruit of Myrobalan medicinal material to 40 meshes, take 500g, and carry out ultrasonic extraction with 2.5L of ethanol with a mass concentration of 10% at 200W, the number of extractions is 1 time, and the extraction time is 30min to obtain the extract ; The extract was concentrated and dried under reduced pressure at 60°C and 0.04MPa to obtain dry extract powder.

[0032] Wherein: the dried fruit of the medicinal material Myrobalan refers to the dried fruit of the plant Myrobalan (Terminalia chebula Retz.).

[0033] (2) Separation and purification: Separation and purification: use n-hexane-ethyl acetate-methanol-water as the solvent system, and the lower phase as the mobile phase, dissolve the crude extract of the target co...

Embodiment 2

[0040] The preparation method of three kinds of chemical reference substances of chebulic acid, chebulic acid and ellagic acid in the medicinal material of Myrobalan chebulae comprises the following steps:

[0041] (1) Extraction: After crushing the dried fruit of Myrobalan medicinal material to 200 meshes, take 500g, and carry out ultrasonic extraction at 400W with 15L of ethanol with a mass concentration of 95%, the number of extractions is 3 times, and the extraction time is 60min to obtain the extract; The extract was concentrated and dried under reduced pressure at 60° C. and 0.04 MPa to obtain dry extract powder.

[0042] Wherein: the dried fruit of the medicinal material Myrobalan refers to the dried fruit of the plant Myrobalan (Terminalia chebula Retz.).

[0043](2) Separation and purification: Separation and purification: use n-hexane-ethyl acetate-methanol-water as the solvent system, and the lower phase as the mobile phase, dissolve the crude extract of the target ...

Embodiment 3

[0049] The preparation method of three kinds of chemical reference substances of chebulic acid, chebulic acid and ellagic acid in the medicinal material of Myrobalan chebulae comprises the following steps:

[0050] (1) Extraction: After crushing the dried fruit of Myrobalan medicinal material to 80 meshes, take 500g, and carry out ultrasonic extraction at 350W with 5L of ethanol with a mass concentration of 70%, the number of extractions is 3 times, and the extraction time is 40min to obtain the extract; The extract was concentrated and dried under reduced pressure at 60° C. and 0.04 MPa to obtain dry extract powder.

[0051] Wherein: the dried fruit of the medicinal material Myrobalan refers to the dried fruit of the plant Myrobalan (Terminalia chebula Retz.).

[0052] (2) Separation and purification: Separation and purification: use n-hexane-ethyl acetate-methanol-water as the solvent system, and the lower phase as the mobile phase, dissolve the crude extract of the target c...

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Abstract

The invention relates to a preparation method of three chemical reference substances, chebulinic acid, chebuligic acid and ellagic acid, in terminalia chebula medicine. The method includes the following steps: 1) crushing dried fruits of terminalia chebula and performing ultrasonic extraction to obtain an extract liquid, and performing pressure-reduced concentration and drying to the extract liquid to obtain a crude extract dried powder; 2) with n-hexane / ethyl acetate / methanol / water as a solvent system, separating the crude extract through HSCCC to respectively obtain the chebulinic acid, the chebuligic acid and the ellagic acid, wherein the purities of the chebulinic acid, the chebuligic acid and the ellagic acid are all more than 95% through purity detection by means of high-performance liquid chromatography (HPLC). The method is simple and quick.

Description

technical field [0001] The invention relates to a preparation method of a chemical reference substance, in particular to a preparation method of three chemical reference substances of chebulic acid, chebulic acid and ellagic acid in chebula medicinal materials. Background technique [0002] Myrobalan (Terminalia chebula Retz.) is a plant of the Junzi family and the main source of medicinal materials of Myrobalan. The main chemical components are polyphenols, galloyl glucose, triterpene acids, etc., which have strong antioxidant, antibacterial and anti-inflammatory , anti-cancer, anti-allergy, inhibition of α-glucosidase activity and other multiple effects, modern pharmacological studies have proved that polyphenols are the main active ingredients. However, gallic acid is currently the chemical component used in the quality control of Myrobalan medicinal materials and its products, which is not enough to fully reflect the quality of Myrobalan. Therefore, the establishment of...

Claims

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Application Information

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IPC IPC(8): C07H13/08C07H1/08C07D493/06
CPCC07H13/08C07D493/06C07H1/08
Inventor 李玉林邹登朗陈涛
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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