Method for preparing D-pantoyl lactone by fermentation

A technology of pantolactone and pantoacid, which is applied in the field of fermentative preparation of D-pantolactone, which can solve the problems of enzyme instability and slow enzyme production

Active Publication Date: 2016-09-21
江西兄弟医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some achievements have been made in the separation of DL-pantolactone by microbial enzymatic methods, there are still some problems to be solved in practical applications, such as slow and less enzyme production in the fermentation process; enzymes are unstable at low pH And other issues

Method used

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Examples

Experimental program
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Effect test

Embodiment approach

[0041] As an embodiment of the present invention, the culture condition is pH 4-9, cultured at 20-40° C. and 100-300 rpm for 0.5-7 days.

[0042] As an embodiment of the present invention, the medium is: 1.5% glycerin, 0.8% peptone, 1.1% yeast extract, 2.5% corn steep liquor, 0.1% spirulina polysaccharide, 0.8% glycine, and the solvent is water.

[0043] As a preferred mode of the present invention, the culture condition is pH 4-7, cultured at 26-29° C. and 100-300 rpm for 0.5-3 days.

[0044] pantothenic acid

[0045] Pantothenic acid, also known as pantothenic acid, vitamin B 5 , widely exists in the biological world, is the main raw material for the synthesis of coenzyme A in animals, and its main function is to participate in the metabolism of sugar, fat and protein. A deficiency in pantothenic acid in the body can cause muscle aches or cramps, adrenal insufficiency and hypofunction, poor concentration, numb hands and feet, constipation, lack of energy, exhaustion aft...

Embodiment 1

[0112] Embodiment 1: a kind of method for preparing D-pantolactone by fermentation, described step comprises:

[0113] (1) cultivating Fusarium oxysporum in culture medium;

[0114] (2) mix the cultured Fusarium oxysporum with the substrate, and hydrolyze to obtain D-pantoic acid;

[0115] (3) The D-pantoic acid of the lactonization step (2) is obtained; wherein,

[0116] The medium is: 1% glycerin, 0.2% peptone, 0.2% yeast extract, 0.5% corn steep liquor, 0.05% spirulina polysaccharide, 0.1% glycine, and the rest is water;

[0117] The conditions of the fermentation culture are: pH 7, cultured at 30°C and 200 rpm for 5 days;

[0118] The immobilization steps are: weigh polyvinyl alcohol and sodium alginate and mix them with water, heat in a water bath and stir until completely dissolved, then let stand and cool to room temperature to obtain a polyvinyl alcohol-sodium alginate mixed solution; Add additives to the sodium alginate mixed solution, then add the wet bacteria, mi...

Embodiment 2

[0126]Embodiment 2: a kind of method for preparing D-pantolactone by fermentation, described step comprises:

[0127] (1) cultivating Fusarium oxysporum in culture medium;

[0128] (2) mix the cultured Fusarium oxysporum with the substrate, and hydrolyze to obtain D-pantoic acid;

[0129] (3) The D-pantoic acid of the lactonization step (2) is obtained; wherein,

[0130] The medium is: 10% glycerin, 2% peptone, 2% yeast extract, 5% corn steep liquor, 0.2% spirulina polysaccharide, 1% glycine, and the rest is water;

[0131] The conditions of the fermentation culture are: pH 7, cultured at 30°C and 200 rpm for 5 days;

[0132] The immobilization steps are: weigh polyvinyl alcohol and sodium alginate and mix them with water, heat in a water bath and stir until completely dissolved, then let stand and cool to room temperature to obtain a polyvinyl alcohol-sodium alginate mixed solution; Add additives to the sodium alginate mixed solution, then add the wet bacteria, mix evenly ...

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Abstract

The invention provides a method for preparing D-pantoyl lactone by fermentation. The method comprises the following steps: culturing fusarium oxysporum in a culture medium; (2) mixing the cultured fusarium oxysporum with a substrate and hydrolyzing to obtain D-pantoic acid; (3) carrying out lactonization on the D-pantoic acid in the step (2) to obtain the D-pantoyl lactone, wherein the culture medium is prepared from 1 percent to 10 percent of glycerol, 0.2 percent to 2 percent of peptone, 0.2 percent to 2 percent of yeast extract, 0.5 percent to 5 percent of maize slurry, 0.05 percent to 0.2 percent of spirulina polysaccharide, 0.1 percent to 1 percent of glycine and the balance of water.

Description

technical field [0001] The invention relates to the field of biochemical industry, and more specifically relates to a method for fermenting and preparing D-pantolactone. Background technique [0002] Pantothenic acid, also known as pantothenic acid, vitamin B 5 , widely exists in the biological world, is the main raw material for the synthesis of coenzyme A in animals, and its main function is to participate in the metabolism of sugar, fat and protein. A deficiency in pantothenic acid in the body can cause muscle aches or cramps, adrenal insufficiency and hypofunction, poor concentration, numb hands and feet, constipation, lack of energy, exhaustion after light exercise, anxiety, nervousness, and teeth grinding. Pantothenic acid is widely used in the fields of medicine, feed and food because of its unique biochemical functions. Its active ingredient is D-configuration D-pantothenic acid, but because pantothenic acid is unstable, its industrial application usually uses deri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/04
CPCC12P17/04
Inventor 周中平张秋华张海峰
Owner 江西兄弟医药有限公司
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