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Method for preparing bacterial cellulose/NBSK (Northern Bleached Softwood Kraft) aerogel spheres under assistance of cation solution

A technology based on bacterial cellulose and cations, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as the collapse of spherical airgel structures, achieve the effect of reducing structure collapse and improving the production rate

Active Publication Date: 2016-09-21
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The difficulty of this technology is that the solvent exchange drying method is inferior to freeze drying and supercritical carbon dioxide drying in terms of retaining the three-dimensional nanostructure of bacterial cellulose, and most of the spherical aerogels will collapse during the drying process.

Method used

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  • Method for preparing bacterial cellulose/NBSK (Northern Bleached Softwood Kraft) aerogel spheres under assistance of cation solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]Acetobacter xylinum was inoculated in 30mL of liquid seed medium (medium composition: fructose 50g / L, peptone 5g / L, yeast extract 5g / L, sodium monohydrogen phosphate 2.6g / L, anhydrous citric acid 1.15g / L L), cultured statically for 48 hours in an incubator at 32°C. Take a 250mL Erlenmeyer flask with a sponge stopper, add 1.25g NBSK fiber, 100mL liquid fermentation medium (the same composition as the seed medium), adjust the pH to 6.0 (the adjustment reagents are 0.1mol / L hydrochloric acid and 0.1mol / L Sodium hydroxide), sterilized at 121°C for 50min (198.64kPa). After the culture medium was cooled, 25 mL of Acetobacter xylinum seed liquid was added, and placed in a shaker with a rotation speed of 150 rpm and a temperature of 32 ° C for 72 hours to obtain bacterial cellulose / NBSK hydrogel balls. The prepared hydrogel spheres were treated with 0.1 mol / L sodium hydroxide solution in a shaker at a temperature of 80° C. and a rotation speed of 100 rpm. Rinse the hydrogel sp...

Embodiment 2

[0040] Acetobacter xylinum was inoculated in 30mL of liquid seed medium (medium composition: fructose 50g / L, peptone 5g / L, yeast extract 5g / L, sodium monohydrogen phosphate 2.6g / L, anhydrous citric acid 1.15g / L L), cultured statically for 48 hours in an incubator at 32°C. Take a 150mL Erlenmeyer flask with a sponge stopper, add 0.9g NBSK, 75mL liquid fermentation medium (the same composition as the seed medium), and adjust the pH value to 6.5 (the adjustment reagents are 0.1mol / L hydrochloric acid and 0.1mol / L Sodium hydroxide), sterilized at 121°C for 50min (198.64kPa). After the medium was cooled, 25 mL of Acetobacter xylinum seed liquid was added, and placed in a shaker with a rotation speed of 200 rpm and a temperature of 32 ° C for 72 hours to obtain bacterial cellulose / NBSK hydrogel balls. The prepared hydrogel spheres were treated with 0.1 mol / L sodium hydroxide solution in a shaker at a temperature of 80° C. and a rotation speed of 100 rpm. Rinse the hydrogel spheres...

Embodiment 3

[0042] Acetobacter xylinum was inoculated in 30mL of liquid seed medium (medium composition: fructose 50g / L, peptone 5g / L, yeast extract 5g / L, sodium monohydrogen phosphate 2.6g / L, anhydrous citric acid 1.15g / L L), cultured statically for 48 hours in an incubator at 32°C. Take a 250mL Erlenmeyer flask with a sponge stopper, add 1.25g NBSK fiber, 100mL liquid fermentation medium (the same composition as the seed medium), and adjust the pH value to 7.0 (adjusting reagents are 0.1mol / L hydrochloric acid and 0.1mol / L Sodium hydroxide), sterilized at 121°C for 50min (198.64kPa). After the culture medium was cooled, 25 mL of Acetobacter xylinum seed liquid was added, and placed in a shaker with a rotation speed of 150 rpm and a temperature of 32 ° C for 72 hours to obtain bacterial cellulose / NBSK hydrogel balls. The prepared hydrogel spheres were treated with 0.1 mol / L sodium hydroxide solution in a shaker at a temperature of 80° C. and a rotation speed of 100 rpm. Rinse the hydro...

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Abstract

The invention relates to a method for preparing bacterial cellulose / NBSK (Northern Bleached Softwood Kraft) aerogel spheres under the assistance of a cation solution. The method comprises the following steps: inoculating acetobacter xylinum into a liquid seed culture medium, and culturing statically to obtain seed liquid; adding NBSK or modified NBSK and a liquid fermentation culture medium into a conical bottle, adjusting the pH to 5.6-7.0, and performing sterilizing treatment; inoculating the seed liquid, and dynamically culturing to obtain the bacterial cellulose / NBSK hydrogel spheres, treating the hydrogel spheres with sodium hydroxide, washing, soaking into deionized water, dropwise adding the cation solution, reacting in a shaking table, adjusting the pH value of the system to an alkaline state, and soaking; taking the hydrogel spheres out, and drying with a solvent to obtain the bacterial cellulose / NBSK aerogel spheres. In the method, large-sized instruments are not needed, and only an ordinary reflux condensation device is needed during drying; the finished product rate of the prepared bacterial cellulose / NBSK aerogel spheres can be up to 50-65 percent.

Description

technical field [0001] The invention belongs to the field of fiber utilization and microbial fermentation, and particularly relates to a method for preparing bacterial cellulose / NBSK airgel balls assisted by a cationic solution. Background technique [0002] Bacterial cellulose is a flexible natural polymer material produced by fermentation of microorganisms under certain conditions. Its cellulose content is as high as 99%, with high crystallinity, high mechanical strength, high porosity, large specific surface area, high water holding capacity, and three-dimensional nano The network structure is an ideal material for preparing cellulose airgel. At present, there are 9 bacterial genera known to be able to synthesize bacterial cellulose, among which Acetobacter xylinum has the highest yield. There are two ways of bacterial cellulose fermentation: dynamic culture and static culture. Under static culture conditions, bacterial cellulose forms a gel-like film on the surface of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08J9/28C12R1/02C08L1/02C08L97/00
CPCC08J9/28C08J2205/026C08J2301/02C08J2397/00C08J2401/02C08J2497/00C12P19/04
Inventor 孟超然郁崇文杨建平张斌郭建生
Owner DONGHUA UNIV
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