Method for extracting anthocyanidin from black chokeberries

A kind of technology of Aronia nigra and anthocyanin, which is applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of reducing anthocyanin decomposition, shortening extraction time, and unfavorable industrial production, so as to shorten production cycle, reduce production costs, and save production costs

Active Publication Date: 2016-09-28
LIAONING FUKANGYUAN BLACK FRUIT OF SORBUS TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Arana berries are rich in anthocyanins, proanthocyanidins, flavonoids and other polyphenols, etc. Its anthocyanin content is more than four times that of blueberries, and proanthocyanidins are more than three times that of blueberries. The total polyphenols are more than 6 times that of blueberries. These substances have various health care and medical effects such as anti-oxidation, anti-aging, lowering blood sugar, anti-mutation and protecting eyesight. Following the research on extracting anthocyanins from blueberries, people now The research work on extracting anthocyanidins and proanthocyanidins from Aronia nigrum was started, but because these works have just started, there are few literature reports on the extraction of anthocyanins from Aronia nigra. Judging from the two existing published articles on the extraction of anthocyanins from Aronia berries, both are based on the method of extracting anthocyanins from blueberries
[0003] Anthocyanins (or anthocyanins) are the general term for anthocyanins and anthocyanins. At present, the methods for extracting anthocyanins all use ethanol solutions with different mass fractions as solvents to extract anthocyanins. These methods have their advantages, but there are also disadvantages. Many shortcomings, such as "China Brewing" 2014 No. 33 published Li Mengsha's "Response Surface Method Optimizing the Ultrasonic Extraction of Aronia Aronia Anthocyanins", which proposed to use 80% volume fraction of ethanol as a solvent, The solid-liquid ratio of Aronia nigricarpa and ethanol solution was 1:10 (g / mL), the extraction temperature was 50°C, and the extraction time was 48 minutes. 3.98%) The advantage of this method is that the anthocyanin has been extracted by the auxiliary measure ultrasonic wave, the ultrasonic wave can accelerate the dissolution of the anthocyanin, shorten the extraction time, and reduce the decomposition of the anthocyanin; the disadvantage is: 1. There is no requirement for the pH value , because the size of the pH value not only determines the structural form of the anthocyanin, but also determines the stability of the anthocyanin; 2. The extraction solvent uses ethanol with a volume fraction of 80%. The coagulation force of the protein is strengthened, which is not conducive to the penetration of the solvent into the cells, and is not conducive to the dissolution of anthocyanins; 3. The aqueous solution of ethanol is used, and the temperature is higher when concentrated. Changes such as decomposition, oxidation, and polymerization are very easy to occur in an atmosphere above 45°C; 4. The author only measured the extraction rate of anthocyanins in the Aronia black fruit extract solution, and did not obtain solid anthocyanins, so this extraction The method is worthless in industrial production, because the anthocyanin is unstable in the solution, and the content of the anthocyanin in the solution decays with the passage of time, how to make the extraction solution of the anthocyanin into a solid with higher content, It is the key to the industrial production of anthocyanins. The solid anthocyanins are not only stable and easy to store, but also easy to transport and apply; 5. The extraction rate is too low, only 3.98%
Its disadvantages are: 1. The ethanol concentration is low, and the water ratio is large, which is not conducive to the extraction of anthocyanins; 2. The ethanol solution has a large water ratio, and the temperature will be high when concentrated, which causes the anthocyanin to be easily decomposed; 3. The ratio of solid to liquid If it is too large, the concentration time will be long, which will not only cause the decomposition of anthocyanins, but also the production cycle will be long, which is not conducive to industrial production; What is obtained is also an extraction solution without further processing into a solid, so industrial production is also impossible with this extraction method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]Embodiment 1: a kind of method that extracts anthocyanin from Aronia nigra, comprises the following steps:

[0031] Step 1: Pulverize the dried fruit of Aronia adenocarpa, pass through a 60-mesh sieve to obtain the dried fruit powder of Aronia adenocarpa, and prepare an azeotropic solution of ethyl acetate and methanol according to the mass ratio of 14:11 (abbreviated as EM reagent ) as an extraction solvent, citric acid and 10% hydrochloric acid solution are formulated into a mixed acidulant according to a mass ratio of 3:2;

[0032] Step 2: Add the dried fruit powder obtained in step 1 into a three-necked flask equipped with a reflux condenser, and add the EM reagent according to the mass ratio of the dried fruit powder to the EM reagent at 1:10, and then add the EM reagent to the three-necked flask according to 5‰ of the quality of the EM reagent. Add the mixed acidifying agent prepared in step 1 to adjust the pH value of the solution, and control the temperature of t...

Embodiment 2

[0036] Embodiment 2: a kind of method that extracts anthocyanin from Aronia nigra, comprises the following steps:

[0037] Step 1: Pulverize the dried fruit of Aronia adenocarpa, pass through a 60-mesh sieve to obtain dried fruit powder, prepare ethyl acetate and methanol azeotropic solution (EM reagent for short) as the extraction solvent according to the mass ratio of 14:11, and Citric acid and 10% hydrochloric acid solution are formulated into a mixed acidulant according to the mass ratio of 3:2;

[0038] Step 2: Add the dried fruit powder obtained in step 1 into the extraction tank equipped with a reflux condenser, and add the EM reagent according to the mass ratio of the dried fruit powder to the EM reagent at 1:10, and then add 5‰ of the EM reagent quality to the solution Add the mixed acidifying agent prepared in step 1, adjust the pH value of the solution to 1-2, and control the temperature of the solution to 40°C and stir for 1 hour, then stop heating and stirring, co...

Embodiment 3

[0042] Embodiment 3: a kind of method that extracts anthocyanin from Aronia nigra, comprises the following steps:

[0043] Step 1: Grinding the dried pomace after squeezing the juice of Aronia berries, passing through a 60-mesh sieve to obtain dried pomace powder, and preparing an azeotropic solution of ethyl acetate and methanol (referred to as EM reagent for short) according to a mass ratio of 14:11 ) as an extraction solvent, citric acid and 10% hydrochloric acid solution are formulated into a mixed acidulant according to a mass ratio of 3:2;

[0044] Step 2: Add the dried pomace powder obtained in step 1 into a three-necked flask equipped with a reflux condenser, and add the EM reagent according to the mass ratio of the dried pomace powder to the EM reagent at 1:10, and then add the EM reagent according to 5‰ of the quality of the EM reagent Add the mixed acidifying agent prepared in step 1 to the three-necked flask, adjust the pH value of the solution to 1-2, and control ...

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Abstract

The invention discloses a method for extracting anthocyanidin from black chokeberries. Azeotropic liquid (an EM reagent for short) of ethyl acetate and methyl alcohol is adopted as an extracting solvent for anthocyanidin in black chokeberries by replacing different ratios of ethanol solutions, both anthocyanidin and anthocyanidin aglycon can be extracted out, and the anthocyanidin extraction rate is increased; meanwhile, when the EM reagent is used as the extracting solvent for anthocyanidin, polarity is appropriate, few impurities with large polarity and few impurities with small polarity are dissolved out, column chromatography isolation and purification are not needed, the anthocyanidin yield is high, and the concentrated extracting solution can be directly subjected to spray drying or vacuum drying to form solid. Accordingly, by means of the method, industrial large-scale production is promoted, meanwhile, the production period is shortened, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of forest food deep processing, and relates to a method for extracting anthocyanins from Aronia nigra. Background technique [0002] Aronia melanocarpa is a species of Aronia melanocarpa in the family Rosaceae. It is native to the northeastern United States and was introduced into my country in the 1990s. Now my country has completed the introduction, breeding, cultivation, and regional experiments. Many areas are promoting planting on a large scale. Arana berries are rich in anthocyanins, proanthocyanidins, flavonoids and other polyphenols, etc. Its anthocyanin content is more than four times that of blueberries, and proanthocyanidins are more than three times that of blueberries. The total polyphenols are more than 6 times that of blueberries. These substances have various health care and medical effects such as anti-oxidation, anti-aging, lowering blood sugar, anti-mutation and protecting eyesight. Follow...

Claims

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Application Information

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IPC IPC(8): C07D311/62C09B61/00C09B67/54
CPCC07D311/62C09B61/00C09B67/0096
Inventor 傅建熙孔东宁王旭太陈世富
Owner LIAONING FUKANGYUAN BLACK FRUIT OF SORBUS TECH DEV
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