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Separation and purification method of sweet corn cob polysaccharide

A technology for separation and purification, sweet corn, applied in the field of sweet corn cob polysaccharide purification, can solve the problems of high polymer non-volatile, unfavorable operation, high viscosity, etc., to achieve the effect of improving the recovery rate and shortening the separation time

Inactive Publication Date: 2016-09-28
HARBIN UNIV OF COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, most of the research on two-phase aqueous phase is limited to the polymer-inorganic salt two-phase system, but because the high polymer is difficult to volatilize and has a high viscosity, it is not conducive to subsequent operations.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] Step 1, preparation of sweet corncob polysaccharide solution: sweet corncobs are dried in an oven at 50°C, crushed with a pulverizer, passed through an 80-mesh sieve, and 1:10 (g:ml) is added to the sieved sweet corncobs with n-hexane (Tianjin Guangfu Fine Chemical Research Institute), magnetically stirred at room temperature for 1 h, centrifuged at 4000 r / min for 10 min, dried and precipitated at room temperature, degreased sweet corn cobs were soaked in hot water at a temperature of 100 ° C, a material-to-liquid ratio of 1:20, and a time of 3 h. extract, centrifuge, and take the supernatant to obtain a sweet corncob crude polysaccharide solution, which is stored at 4°C for later use.

[0019] Step 2, configuration of the two-phase aqueous system: according to the isopropanol-ammonium sulfate two-phase phase diagram, add a certain amount (0.25g / ml) of ammonium sulfate (Tianjin Bohai Chemical Co., Ltd.) to the 25mL colorimetric tube, Then add an appropriate amount of sw...

Embodiment 1

[0024] According to the isopropanol-ammonium sulfate two-phase phase diagram, add 5.00 g of ammonium sulfate (Tianjin Bohai Chemical Co., Ltd.) into the 25mL colorimetric tube, and then add an appropriate amount of sweet corncob polysaccharide solution. After the ammonium sulfate is completely dissolved, finally Add isopropanol (Tianjin Bohai Chemical Co., Ltd.) with a volume fraction of 40% to 20 mL to form an aqueous two-phase system. After standing still to form a clear phase, place it in an ultrasonic wave, and treat it for 25 minutes under the conditions of 25°C, 500w, and 45HZ, and separate the phases in a separating funnel, measure the volume of the upper and lower phases and the protein and polysaccharide content of the upper and lower phases, and calculate Comparison, protein partition coefficient, polysaccharide partition coefficient and recovery. The determination of protein content adopts Bradford method (Coomassie brilliant blue is produced by Shanghai Ruji Biotec...

Embodiment 2

[0026] According to the isopropanol-ammonium sulfate two-phase phase diagram, add 3.50 g of ammonium sulfate (Tianjin Bohai Chemical Co., Ltd.) into the 25mL colorimetric tube, and then add an appropriate amount of sweet corncob polysaccharide solution. After the ammonium sulfate is completely dissolved, finally Add isopropanol (Tianjin Bohai Chemical Co., Ltd.) with a volume fraction of 30% to 20 mL to form an aqueous two-phase system. After standing still to form a clear phase, place it in an ultrasonic wave and treat it under the conditions of 25°C, 500w, and 45HZ for 15 minutes, then stand still in a separating funnel to separate the phases, measure the volume of the upper and lower phases and the protein and polysaccharide content of the upper and lower phases, and calculate Comparison, protein partition coefficient, polysaccharide partition coefficient and recovery. The determination of protein content adopts Bradford method (Coomassie brilliant blue is produced by Shang...

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Abstract

The invention discloses a separation and purification method of sweet corn cob polysaccharide. The separation and purification method comprises the following steps of drying and crushing sweet corn cobs, adding normal hexane into sieved sweet corn cobs according to the weight volume ratio being 1: (8 to 12), performing magnetic stirring under the room temperature condition for 1 to 1.5 hours, and centrifuging at the speed of 4000r / min for 10 to 15 minutes; performing hot water extraction and centrifuging on degreased sweet corn cobs under the conditions that the temperature is 100DEG C, the material-to-liquid ratio is 1:(18 to 22), and the time is 2.5 to 3.5 hours, and taking supernate; adding 3 to 5g of ammonium sulfate into a 25mL colorimetric tube according to an isopropanol-ammonium sulfate aqueous two-phase diagram, and then adding sweet corn cob crude polysaccharide liquid; after the ammonium sulfate is completely dissolved, finally adding the isopropanol until the volume is 20ml, so as to form an aqueous two-phase system solution; enabling the aqueous two-phase system solution processed by ultrasonic waves to stand in a separating funnel for phase separation; respectively determining the volumes of upper and lower phases, and the contents of polysaccharides and protein of the upper and lower phases; preserving the polysaccharide solution obtained after separating and purifying two aqueous phases at 4DEG C in a refrigerator for 1 to 2 days; after ammonium sulfate crystals are separated out, dialyzing and performing rotary evaporation and concentration to small volume; finally, performing vacuum freeze drying, thereby obtaining the sweet corn cob polysaccharide.

Description

technical field [0001] The invention relates to a method for separating and purifying sweet corn cob polysaccharides, and belongs to the technical field of purification methods for sweet corn cob polysaccharides. Background technique [0002] Sweet corn is the sweet quality of the genus Maize, which is a variety of corn and is called vegetable corn by Western Europe. The content of polysaccharides in sweet corncobs is relatively high, and sweet corncob polysaccharides are a multi-component water-soluble polysaccharide, which contains a small amount of acidic sugars. Sweet corn cob polysaccharide has beneficial biological activities such as anticoagulant, antioxidation, hypoglycemic and hypolipidemic, and can be used as a drug for development and utilization. The polysaccharides obtained from sweet corncobs after hot water extraction contain impurities such as proteins and pigments. Therefore, it is necessary to find a way to separate sweet corncob polysaccharides from impur...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 马永强王鑫高爽谢静南庄星光
Owner HARBIN UNIV OF COMMERCE
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