Unlock instant, AI-driven research and patent intelligence for your innovation.

Production method of feed flavoring agent containing rich small peptides

A production method and technology for flavoring agents are applied in the field of feed flavoring agents rich in small peptides, which can solve the problems of high protein and poor palatability, and achieve the effects of high-efficiency enzymatic hydrolysis and good feeding effect.

Inactive Publication Date: 2016-10-12
源耀生物科技(盐城)股份有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Corn gluten meal is a by-product of the production of corn starch. It has the characteristics of high protein and poor palatability, and its application in feed is greatly restricted.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the production method of the feed flavoring agent that is rich in small peptides

[0032] Step S1, prepare corn gluten powder suspension: make corn gluten powder into a suspension with a mass percent concentration of 10% with water, adjust the pH value of the suspension to 9.0, and raise the temperature to 55°C while stirring; adjust the pH of the suspension forward Add 5% Ophiopogon japonicus extract in the suspension;

[0033] Step S2, enzymatic hydrolysis: add alkaline protease to the suspension according to the ratio of 200IU / g of material, and then add neutral protease according to the ratio of 200IU / g of material, and enzymolyze to pH 5.5 ;

[0034] Step S3, filtering: filter the above enzymolysis solution with a ceramic membrane and a reverse osmosis membrane in sequence, filter out components with a molecular weight greater than 1000 Da, and collect the filtrate to obtain a filtrate rich in small peptides;

[0035] Step S4, fermentation: adding g...

Embodiment 2

[0043] Embodiment 2: the contrast of embodiment 1, do not add Ophiopogon japonicus extract

[0044] Step S1, preparing corn gluten powder suspension: making corn gluten powder into a suspension with a concentration of 10% by mass with water, adjusting the pH value of the suspension to 9.0, and raising the temperature to 55° C. while stirring;

[0045] Step S2, enzymatic hydrolysis: add alkaline protease to the suspension according to the ratio of 200IU / g of material, and then add neutral protease according to the ratio of 200IU / g of material, and enzymolyze to pH 5.5 ;

[0046] Step S3, filtering: filter the above enzymolysis solution with a ceramic membrane and a reverse osmosis membrane in sequence, filter out components with a molecular weight greater than 1000 Da, and collect the filtrate to obtain a filtrate rich in small peptides;

[0047] Step S4, fermentation: adding glucose with a mass volume percentage of 2.0% to the above-mentioned filtrate rich in small peptides, ...

Embodiment 3

[0051] Embodiment 3: comparative example, Ophiopogon japonicus is not through steaming

[0052] Step S1, prepare corn gluten powder suspension: make corn gluten powder into a suspension with a mass percent concentration of 10% with water, adjust the pH value of the suspension to 9.0, and raise the temperature to 55°C while stirring; adjust the pH of the suspension forward Add 5% Ophiopogon japonicus extract in the suspension;

[0053] Step S2, enzymatic hydrolysis: add alkaline protease to the suspension according to the ratio of 200IU / g of material, and then add neutral protease according to the ratio of 200IU / g of material, and enzymolyze to pH 5.5 ;

[0054] Step S3, filtering: filter the above enzymolysis solution with a ceramic membrane and a reverse osmosis membrane in sequence, filter out components with a molecular weight greater than 1000 Da, and collect the filtrate to obtain a filtrate rich in small peptides;

[0055] Step S4, fermentation: adding glucose with a m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a production method of a feed flavoring agent containing rich small peptides. The production method comprises steps as follows: preparation of a corn gluten meal suspension: the suspension is prepared from corn gluten meal, the pH value of the suspension is adjusted to range from 8.8 to 9.2, and the mixture is heated to 53-57 DEG C while stirred; enzymolysis: alkaline protease is added to the suspension, the suspension is subjected to enzymolysis until the pH value ranges from 6.5 to 7.0, then, neutral protease is added, and the mixture is subjected to enzymolysis until the pH value ranges from 5.3 to 5.7; filtration: the solution after enzymolysis is sequentially filtered by a ceramic membrane and a reverse osmosis membrane, ingredients with the molecular weight larger than 1,000 Da are filtered out, a filtrate is collected, and the filtrate containing the rich small peptides is obtained; fermentation: glucose is added to the filtrate containing the rich small peptides, and after glucose is dissolved, saccharomyces cerevisiae is added for aerobic fermentation for 70-74 h; wall breaking: lyticase is added to fermentation broth to break walls of the saccharomyces cerevisiae; homogeneous drying: the fermentation broth after wall breaking is treated in a homogenizer, and a finished product is obtained after spray drying. The feed flavoring agent produced with the production method contains rich small peptides, further contains rich nucleotide and has a good attractant effect.

Description

technical field [0001] The invention relates to a feed, in particular to a production method of a feed flavoring agent rich in small peptides. Background technique [0002] As the main digestion product of protein, small peptides play an important role in the process of amino acid digestion, absorption and nutrient metabolism. Small peptides with 2-4 amino acids can be quickly absorbed by the small intestinal villous epithelial cells, and the absorption speed and absorption rate are higher than those of macromolecular proteins and amino acids. At the same time, some small peptides have special physiological functions, such as anti-pathogenic bacteria, promoting fat metabolism, promoting mineral absorption, and have a good antioxidant function. Small peptides are also widely used in feed as a feed additive. Corn gluten meal is a by-product of the production of corn starch. It has the characteristics of high protein and poor palatability, and its application in feed is great...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23K20/147A23K20/153A23K10/14A23K10/12A23K10/30C12P21/06
CPCC12P21/06
Inventor 方华季春源孙中超肖方方郭蓓蓓
Owner 源耀生物科技(盐城)股份有限公司