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Extraction method of purple corn anthocyanin

An extraction method, the technology of purple corn, applied in the direction of organic chemistry, can solve the problems of high toxic and side effects of organic solvents, low extraction efficiency, time-consuming operation, etc., and achieve high product quality, fast extraction speed, and less waste liquid discharge Effect

Inactive Publication Date: 2016-10-12
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, the extraction process of anthocyanins in purple corn mostly adopts traditional methods such as hot water extraction method or direct acid hydrolysis extraction method, organic solvent extraction method to extract anthocyanins from purple corn, but when these traditional methods extract pigments , organic solvents have large toxic and side effects, and the extraction efficiency is low, the operation is time-consuming, energy-consuming, and the extraction cost is high

Method used

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  • Extraction method of purple corn anthocyanin
  • Extraction method of purple corn anthocyanin
  • Extraction method of purple corn anthocyanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Extraction of Anthocyanins from Jinheiyu No. 2 Corn Kernels

[0057] The grains of Jin Heiyu No. 2 were used as raw materials, dried at 35°C at low temperature, and then crushed into 40-mesh fine powder. 10.0 g of the crushed Jin Heiyu No. 2 corn fine powder was accurately weighed, placed in a conical flask, and The mixed solution of 95% ethanol, 0.1mol / L citric acid and distilled water with a volume ratio of 4:1:3 is used as the extraction solution, and 100ml of the extraction solution is added to the conical flask. Extract anthocyanins from Jinheiyu No. 2 corn fine powder for 30 minutes under the assistance of ultrasonic waves with a power of 400W; centrifuge the mixture after ultrasonic-assisted extraction at a speed of 8000 rpm / min for 10 minutes, and then separate the filter residue and filtrate , carry out secondary extraction, the extraction time is halved, centrifuged at a speed of 8000rpm / min again, and the two filtrates are mixed; the filtrate is vacuum concen...

Embodiment 2

[0066] Extraction of Anthocyanins from Zhuozi No.1 Corn Kernel

[0067] Use Zhuozi No. 1 corn grains as raw materials, dry them at 35°C at low temperature, and then crush them into 60-mesh fine powder. Accurately weigh 10 g of the crushed Zhuozi No. 1 corn fine powder, place them in a conical flask, and measure Be the mixed solution of 90% ethanol, 0.2mol / L citric acid and distilled water of 4: 2: 5 as extracting solution, add the extracting solution of 100ml in the Erlenmeyer flask, after mixing evenly, at extraction temperature 45 ℃, ultrasonic power is Under the assisted action of 450W ultrasonic waves, anthocyanins were extracted from Zhuozi No. 1 corn fine powder for 35 minutes; The second extraction, the extraction time was halved, centrifuged again at a speed of 8000rpm / min, and the two filtrates were mixed; the filtrate was vacuum concentrated in a rotary evaporator, and the operating conditions were temperature 40°C and vacuum degree 0.04MPa to obtain a concentrated s...

Embodiment 3

[0076] Extraction of anthocyanins from purple pearl corn kernels

[0077]Use purple pearl purple corn grains as raw materials, dry at 35°C at low temperature, and then crush them into 20-mesh fine powder. Accurately weigh 20.0g of the crushed purple pearl purple corn fine powder and place them in a conical flask. The mixture of 95% ethanol, 0.3mol / L citric acid and distilled water of 4:1:2 is used as the extraction solution, add 100ml of the extraction solution to the Erlenmeyer flask, after mixing evenly, at the extraction temperature of 35°C, the ultrasonic power is 350W Under the assisted action of ultrasonic waves, anthocyanins were extracted from purple pearl purple corn fine powder for 45 minutes; the mixture after ultrasonic assisted extraction was centrifuged at a speed of 10,000 rpm / min for 5 minutes, and then the filter residue and filtrate were separated for secondary extraction , the extraction time is halved, centrifuged again at a speed of 10000rpm / min, and the t...

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Abstract

The invention provides a method for extracting anthocyanins from purple corn. Specifically, purple corn grains are used as raw materials. After pretreatment, the anthocyanins in purple corn grains are first roughly extracted, followed by ultrafiltration and nanofiltration. , macroporous resin adsorption, ethanol elution, secondary nanofiltration, and vacuum freeze-drying to obtain purple corn anthocyanins. The purple corn anthocyanin extraction method provided by the present invention has good selectivity, can effectively separate impurities such as polysaccharides, organic acids and proteins in purple corn anthocyanins, has high extraction rate, high product quality, and can be extracted even in small amounts. The extraction speed is fast, the operation is simple, and the waste liquid discharge is small.

Description

technical field [0001] The invention relates to the technical field of extracting plant functional natural active ingredients, in particular to a method for extracting purple corn anthocyanins. Background technique [0002] Anthocyanidin (Anthocyanidin) widely exists in the plant kingdom, its color is bright, non-toxic, red or purple in acidic conditions, blue in alkaline conditions. It is a water-soluble pigment belonging to flavonoids in phenolic compounds. The basic structure consists of two benzene rings connected by a 3-carbon unit (C6-C3-C6). Anthocyanins are produced by the phenylpropionic acid pathway and the flavonoid biosynthetic pathway, which are catalyzed by the regulation of many enzymes. In the natural state, anthocyanins combine with various monosaccharides in plants to form anthocyanins. Anthocyanin is a powerful antioxidant that can protect the human body from free radical damage, enhance blood vessel elasticity, improve the circulatory system, inhibit i...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 马超周爽庞玉辉张均贾琦石李友军
Owner HENAN UNIV OF SCI & TECH
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