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Preparation method of plant lactobacillus microcapsule

A technology of plant lactic acid bacteria and microcapsules, which is applied in the field of preparation of plant lactic acid bacteria microcapsules, can solve the problems of lactic acid bacteria activity destruction and poor tolerance of the microcapsule stomach environment, and achieve simple operation, good tolerance and high activity of lactic acid bacteria Effect

Inactive Publication Date: 2016-10-26
丁玉琴
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem mainly solved by the present invention: the cross-linking agent used in the preparation process of the currently prepared lactic acid bacteria microcapsules has certain toxicity, which will have a greater destructive effect on the activity of lactic acid bacteria, and the prepared microcapsules are harmful to the stomach. To solve the problem of poor environmental tolerance, a method for preparing plant lactic acid bacteria microcapsules is provided. In this method, single colonies are picked after shaking the soymilk for Gram staining and microscopic examination, and the obtained Gram-positive strains are further tested. Cultivate and centrifuge to obtain lactic acid bacteria strains, then stir the kelp powder with cellulase and calcium chloride, and centrifuge to obtain the lower layer of precipitate, then stir and react with hydrochloric acid solution and sodium hydroxide solution, and filter the obtained gel and oligomer Fructose, the above-mentioned lactic acid bacteria strains, etc. are stirred and reacted, after being atomized, they are left to solidify together with the calcium chloride solution, filtered to obtain gelatinous microcapsules, and a kind of plant lactic acid bacteria microgels can be obtained by drying the microcapsules. It is easy to operate, no cross-linking agent is used in the preparation process, and will not cause any damage to the activity of lactic acid bacteria, and the prepared microcapsules have good tolerance to the gastric environment and have broad market prospects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022]First weigh 100g of soybean milk and add it to a petri dish, stir evenly, place it in a shaker, shake at a speed of 180r / min for 40min at room temperature, then transfer it to a lactic acid bacteria screening medium, and then place it in an anaerobic incubator , cultured at 36°C for 24 hours, picked a single colony for Gram staining, and microscopic examination to obtain Gram-positive strains; then inoculated the Gram-positive strains on the lactic acid bacteria screening medium at an inoculum size of 10%. , placed in an anaerobic incubator, at 36°C, continued to cultivate for 36h, centrifuged the culture solution at 6000r / min for 10min, discarded the supernatant to obtain lactic acid bacteria strains, and set aside; then take 30g of fresh kelp and use Wash it with deionized water, dry it naturally, and grind it with a pulverizer. Put the kelp powder into a beaker, add 100mL deionized water and 0.01g cellulase, and then add 100mL of disodium hydrogen phosphate and diphosp...

example 2

[0026] First weigh 150g of soybean milk and add it to a petri dish, stir evenly, place it in a shaker, shake at a speed of 190r / min for 45min at room temperature, then transfer it to a lactic acid bacteria screening medium, and then place it in an anaerobic incubator , cultured at 37°C for 24.5 hours, picked a single colony for Gram staining, and microscopic examination to obtain Gram-positive strains; then inoculated the Gram-positive strains into the lactic acid bacteria screening medium according to the inoculum size of 10%. Put it in an anaerobic incubator, continue to cultivate at 37°C for 42h, centrifuge the culture solution at 7000r / min for 15min, discard the supernatant, and obtain lactic acid bacteria strains for later use; then take 35g of fresh kelp and use Wash it with deionized water, dry it naturally, and pulverize it with a pulverizer. Add kelp powder to a beaker, add 150mL deionized water and 0.02g cellulase, and then add 150mL of disodium hydrogen phosphate and...

example 3

[0030] First weigh 200g of soybean milk and add it to a petri dish, stir evenly, place it in a shaker, shake at a speed of 200r / min for 50min at room temperature, then transfer it to a lactic acid bacteria screening medium, and then place it in an anaerobic incubator , cultured at 38°C for 25 hours, picked a single colony for Gram staining, and microscopic examination to obtain Gram-positive strains; then inoculated the Gram-positive strains on the lactic acid bacteria screening medium at an inoculum size of 10%. , placed in an anaerobic incubator, at 38°C, continued to cultivate for 48h, centrifuged the culture solution at 8000r / min for 20min, discarded the supernatant to obtain lactic acid bacteria strains, and set aside; then take 40g of fresh kelp and use Wash it with deionized water, dry it naturally, and grind it with a pulverizer. Put the kelp powder into a beaker, add 200mL deionized water and 0.03g cellulase, and then add 200mL of disodium hydrogen phosphate and dibasi...

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PUM

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Abstract

The invention discloses a method for preparing plant lactic acid bacteria microcapsules, which belongs to the technical field of microcapsule preparation. In the present invention, a single colony is picked after soymilk shake culture and carried out Gram staining and microscopic examination, and the obtained Gram-positive strains are further cultured and centrifuged to obtain lactic acid bacteria strains, and then the kelp powder is mixed with cellulase, chlorinated Stir and centrifuge the calcium, etc. to get the lower layer of precipitate and then stir and react with hydrochloric acid solution and sodium hydroxide solution. The gel obtained by filtration is stirred and reacted with fructooligosaccharides and the above-mentioned lactic acid bacteria strains. After atomization, it is mixed with calcium chloride solution Stand to solidify, filter to obtain gelatinous microcapsules, dry the microcapsules to obtain a plant lactic acid bacteria microgel, the invention is easy to operate, no cross-linking agent is used in the preparation process, and will not cause any damage to the activity of lactic acid bacteria , and the prepared microcapsules have good tolerance to gastric environment and have broad market prospects.

Description

technical field [0001] The invention discloses a method for preparing plant lactic acid bacteria microcapsules, which belongs to the technical field of microcapsule preparation. Background technique [0002] The wide application of antibiotics and other antibacterial drugs in animal husbandry has greatly promoted the development of animal husbandry. But its disadvantages are also becoming more and more obvious: serious drug residues in animal husbandry products directly endanger human health; the abuse of antibiotics leads to drug resistance of pathogenic bacteria, resulting in the spread and spread of drug-resistant strains; at the same time, animal flora imbalance, animal immunity decline, the breeding environment deteriorates, and diseases increase; causing a series of serious problems such as animal endogenous infection and double cross-infection. At present, the standardization and reduction of the use of antibacterial drugs in the breeding process, and the development...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K40/30
Inventor 丁玉琴孟浩影薛蕾
Owner 丁玉琴
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