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High-solubility probiotics fermentation dendrobium nobile powder and preparation and application thereof

A high solubility, probiotic technology, applied in the direction of bacteria, application, and food ingredients used in food preparation as solubility improvers, etc., can solve the problems of poor solubility, rough taste, easy stratification in brewing, etc., and achieve a delicate taste. , the effect of improving flavor, reliable safety

Inactive Publication Date: 2016-10-26
杭州彧弋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a preparation process of highly soluble probiotic fermented dendrobium powder and its fermentation product and application, aiming to solve the problem of dissolving the existing probiotic fermented dendrobium powder by pretreating dendrobium with cellulase before fermentation Problems such as poor performance, easy stratification after brewing, rough taste, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) After fresh Dendrobium officinale is collected, remove impurities and clean it in time, cut it into 1-2cm segments, crush it to 20 meshes, and obtain 100g of pretreated Dendrobium officinale;

[0026] 2) Add sterile water to the pretreated Dendrobium officinale according to the mass ratio of 1:10, pass through a colloid mill (JMS-240, Langfang Tongyi Machinery Co., Ltd.) for colloidal treatment, and make a dendrobium homogenate, using 60 Co gamma radiation sterilization method (dose 9kGy) carries out sterilizing treatment to dendrobium homogenate, obtains the dendrobium candidum homogenate 1100g after sterilization;

[0027] 3) Add 1 g of cellulase (enzyme activity 200 U / mg) to the sterilized homogenate of Dendrobium candidum at a mass concentration of 0.1% based on the mass of the pretreated dendrobium to carry out enzymatic hydrolysis;

[0028] 4) The enzymolysis temperature is 35°C, the rotation speed is 80r / min, and the enzymolysis time is 1.5h, and the homogenat...

Embodiment 2

[0040] 1) Impurities removed and cleaned in time after collection of Dendrobium Huoshanense, cut into 1-2cm segments, crushed to 20 mesh, and 100g of pretreated Dendrobium was obtained;

[0041] 2) The pretreated Dendrobium Huoshanense was added sterile water according to the ratio of mass ratio 1:30, and carried out colloidal treatment (same as Example 1) through a colloid mill to make a dendrobium homogenate; 60 Co gamma radiation sterilization method (dose 9kGy) carries out sterilizing treatment to dendrobium homogenate, obtains the dendrobium homogenate 3100g after sterilization;

[0042] 3) Adding 0.4 g of cellulase (enzyme activity 200 U / mg) to the sterilized dendrobium homogenate based on the mass of the pretreated dendrobium at a ratio of 0.4% for enzymatic hydrolysis;

[0043] 4) The enzymolysis temperature is 40°C, the rotation speed is 100r / min, and the enzymolysis time is 1h to obtain the dendrobium homogenate after enzymolysis;

[0044] 5) Add 7g of light calcium...

Embodiment 3

[0055] 1) After collecting Dendrobium nobile, remove impurities in time, clean it, cut it into 1-2cm segments, crush it to 20 mesh, and obtain 100g of pretreated Dendrobium;

[0056] 2) Add sterile water to the pretreated Dendrobium nobile in a ratio of 1:50 in mass ratio, and carry out colloidal treatment (same as Example 1) through a colloid mill to make a dendrobium homogenate; 60 Co gamma radiation sterilization method (dose 9kGy) carries out sterilizing treatment to dendrobium homogenate, obtains the dendrobium nobile homogenate 5110g after the sterilization;

[0057] 3) Adding 1 g of cellulase (enzyme activity 200 U / mg) to the sterilized Dendrobium homogenate at a mass concentration of 1% based on the mass of the pretreated Dendrobium to perform enzymatic hydrolysis;

[0058] 4) The enzymolysis temperature is 50°C, the rotation speed is 150r / min, and the enzymolysis time is 0.5h;

[0059] 5) Add 5g of light calcium carbonate to the dendrobium homogenate after enzymolysi...

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Abstract

The invention discloses high-solubility probiotics fermentation dendrobium nobile powder and preparation and application thereof. Cellulase is utilized to perform enzymolysis on dendrobium nobile before fermentation, the solubility and palatability of dendrobium nobile are improved, and carbohydrates generated by cellulase enzymolysis can serve as a substrate for fermentation of lactic acid bacteria; after the fermentation, the number of live bacteria of probiotics can be 109 CFU / g or above; the fermented dendrobium nobile powder is good in solubility, is not subjected to layering when brewed, and is fine in taste. According to the technology, the shortcomings that existing probiotics fermentation dendrobium nobile powder is poor in solubility, is subjected to layering after being brewed and is coarse in taste are overcome. The dissolving mass percentage of the fermented dendrobium nobile powder can reach 94% or above, and the solubility and palatability of the dendrobium nobile are remarkably improved.

Description

(1) Technical field [0001] The invention relates to the preparation of a biologically fermented dendrobium powder, and relates to a preparation process of a highly soluble probiotic fermented dendrobium powder, a fermentation product and an application thereof. (2) Background technology [0002] Dendrobium nobile Lindl (scientific name: Dendrobium nobile Lindl), also known as Wanzhangxu, Chlorophytum, Lindl, Forbidden Life, Nobile Flower and so on. Medicinal plant, sweet and slightly salty in nature and flavor, cold, and returns to the stomach, kidney, and lung meridian. Tonify stomach and promote body fluid, nourish yin and clear away heat. It is used for yin deficiency and body fluid deficiency, dry mouth, polydipsia, retching due to lack of food, asthenia heat after illness, and blurred vision. Dendrobium candidum ranks first among the "Nine Immortal Herbs", and other species such as Huoshan Dendrobium and Dendrobium nobile are traditional and precious Chinese herbal me...

Claims

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Application Information

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IPC IPC(8): A23L33/00A23L29/30A23L29/00A23L33/10
CPCA23V2002/00A23V2400/165A23V2400/125A23V2400/123A23V2400/113A23V2400/169A23V2200/14A23V2200/238A23V2200/30A23V2250/6402
Inventor 杨善岩吴瑶瑶夏亚娟
Owner 杭州彧弋科技有限公司
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