Free radical degradation product of abelmoschus manilhot stem leaf polysaccharide capable of improving immunocompetence and preparation method thereof

A technology of degradation product, hollyhock, applied in the direction of organic active ingredients, medical preparations containing active ingredients, drug combinations, etc. The effect of continuous application

Active Publication Date: 2016-10-26
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign studies on polysaccharides from stems and leaves of hollyhock mainly focus on the analysis of physical and chemical properties and monosaccharides, and there are few studies on its activity.

Method used

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  • Free radical degradation product of abelmoschus manilhot stem leaf polysaccharide capable of improving immunocompetence and preparation method thereof
  • Free radical degradation product of abelmoschus manilhot stem leaf polysaccharide capable of improving immunocompetence and preparation method thereof
  • Free radical degradation product of abelmoschus manilhot stem leaf polysaccharide capable of improving immunocompetence and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of the free radical degradation product of the stem and leaf polysaccharide of Abelmoschus manihot according to the present invention includes the following steps:

[0032] (1) Preparation of crude polysaccharides from stems and leaves of Abelmoschus manihot

[0033] 100g of coarse powder of hollyhock stems and leaves were added with 30 times the amount of water, and extracted 3 times with reflux in a water bath at 100°C, each for 1 hour. Filter, combine the filtrates, and concentrate under reduced pressure. Remove protein by Sevag method, centrifuge (4000r / min, 10min), take the supernatant, add four times the volume of absolute ethanol, precipitate with alcohol overnight, filter with suction, and wash the precipitate three times with absolute ethanol, acetone, and ether, 50℃ After drying, crude polysaccharides from stems and leaves of hollyhock are obtained.

[0034] (2) Grading of crude polysaccharides from hollyhock stems and leaves

[0035] Weigh 3g...

Embodiment 2

[0038] Example 2 Determination of the total sugar and uronic acid content of the free radical degradation products of the stem and leaf polysaccharide of Abelmoschus manihot

[0039] 1. Experimental method: Using D-anhydrous glucose as the standard product, the total sugar content of hollyhock stem and leaf polysaccharides before and after sulfation modification was determined by the phenol-sulfuric acid method; the uronic acid content was determined by the meta-hydroxybiphenyl method, and D-glucose Aldehydic acid is the standard product.

[0040] 2. Experimental results: The total sugar content of hollyhock stem and leaf polysaccharide prepared in Example 1 was 99.76%. The total sugar content of free radical degradation products of hollyhock stem and leaf polysaccharide decreased to 95.53%; the hollyhock stem and leaf polysaccharide was not degraded before degradation. Contains uronic acid and no uronic acid after degradation, which is consistent with the result of monosaccharide ...

Embodiment 3

[0041] Example 3 Analysis of monosaccharide composition of polysaccharides from stems and leaves of Abelmoschus manihot and its free radical degradation products

[0042] 1. The experimental method uses trifluoroacetic acid to hydrolyze polysaccharide samples, with mixed monosaccharides as the standard, PMP-derived polysaccharide samples and mixed standards, using high performance liquid chromatography to determine the composition of monosaccharides according to the peak time, and draw each monosaccharide based on the peak area The standard curve of sugar measures the monosaccharide content of the sample.

[0043] 2. Experimental results: analysis of monosaccharide composition

[0044] The molar ratio of the monosaccharide composition of the stem and leaf polysaccharide of hollyhock and its degradation products prepared in Example 1 is shown in Table 1. The stem and leaf polysaccharide of hollyhock is composed of mannose, glucose, galactose and arabinose. Among them, the content of g...

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Abstract

The invention discloses a free radical degradation product of abelmoschus manilhot stem leaf polysaccharide and a preparation method thereof. The free radical degradation product of the abelmoschus manilhot stem leaf polysaccharide is composed of mannose, glucose, galactose and arabinose in molar ratio of 0.34:18.56:1.0:0.36 and has the molecular weight of 552.36kDa. The preparation method comprises the following steps: optimizing an extraction separation technology by virtue of a large number of experiments, carrying out water extraction and alcohol precipitation for obtaining crude polysaccharide, then carrying out deproteinization, then purifying by adopting DEAE-52 celluosic resin, so as to obtain high-purity abelmoschus manilhot stem leaf polysaccharide, then degrading by adopting an H2O2-Vc system, and purifying, so that the free radical degradation product of the abelmoschus manilhot stem leaf polysaccharide is obtained. The preparation method disclosed by the invention has the advantages that abandoned abelmoschus manilhot stem leaf resource is fully utilized, waste materials are changed into things of value, the free radical degradation product of the abelmoschus manilhot stem leaf polysaccharide capable of improving immunocompetence is obtained, and sustainable application of traditional Chinese medicine resources can be realized, so that the preparation method has good economic value and great significance for ecological environmental protection.

Description

Technical field [0001] The invention relates to a plant polysaccharide, in particular to a free radical degradation product of the hollyhock stem and leaf polysaccharide with improved immune activity and a preparation method thereof. Background technique [0002] Traditional Chinese medicine resources are a solid foundation for the protection of national health and the development of national medicine. In recent years, the production area of ​​traditional Chinese medicine and natural medicinal biological resources has exceeded 2.40×10 6 hm 2 , The output of medicinal materials can reach 5.40×10 6 t, and the biomass of abandoned plant roots and above-ground stems and leaves is as high as 1.1×10 7 ~1.6×10 7 t is 2 to 3 times the output of medicinal materials, causing serious waste of resources and environmental pollution. Therefore, in the process of industrialization of traditional Chinese medicine resources, improving the utilization value of medicinal biological resources, espec...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P37/04A61K36/185
CPCA61K31/715A61K36/185C08B37/0003C08B37/006
Inventor 江曙潘欣欣段金廒钱大玮朱悦严辉
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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