Composition for UV protection containing hijiki extract as an active ingredient

A technology of extract and hijiki, applied in cosmetic preparations, preparations for skin care, pharmaceutical formulations, etc., can solve the problems of clogging pores, side effects, chapped skin, etc., and achieve the effect of ultraviolet isolation.

Active Publication Date: 2019-09-10
人类科学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although UV-B only reaches the epidermis of the skin, it can also induce erythema, blisters, chapped skin, wrinkles and even skin cancer if exposed to it for a long time
In order to effectively isolate this kind of ultraviolet rays, inorganic ultraviolet scattering agents or organic ultraviolet absorbers are often used, but they will also clog pores and cause various side effects. cause of inflammation

Method used

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  • Composition for UV protection containing hijiki extract as an active ingredient
  • Composition for UV protection containing hijiki extract as an active ingredient
  • Composition for UV protection containing hijiki extract as an active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Extraction of phlorotannins from Hijiki

[0050] The hijiki used in the present invention was collected in the East China Sea from December 2012 to January 2013, and after using tap water to remove salt and sand, it was naturally air-dried in a cool place, crushed and stored. The evidence specimens were kept in the National Biological Resource Museum (specimen voucher: NIBRAL0000137941), using existing methods (Fairhead et al., Polar Biol28: 680-686.2005; Lopes et al., PLoS ONE 7: e31145.2012) to extract brown algae polyphenol extracts in the following manner ( phlorotannins extract, hereinafter referred to as "PE").

[0051] First, immerse 100 g of the above-mentioned broken hijiki in 300 mL of n-hexane (n-hexane) twice, remove the supernatant, remove the lipid and evaporate the solvent, and then immerse the remaining residue in 500 ml of 70% acetone aqueous solution again , extracted at room temperature for 24 hours, filtered using filter paper (Whatmann I...

Embodiment 2

[0052] Embodiment 2: DMBA analysis

[0053] In order to quantitatively measure the polyphenol extract (PE) of brown algae extracted according to an embodiment of the present invention, DMBA analysis was performed.

[0054] Specifically, phloroglucinol (phloroglucinol, Sigma-Aldrich, MO, USA) was used as a standard substance (standard substance) calculated for relative comparison. For relative volume, PE was dissolved in distilled water to reach 62.5-1000 μg, then, the final volume of all samples was adjusted to 10 μl, 10 μl of methanol was added, 10 μl of N,N-dimethylformamide (DMF) was added and acetic acid ( acetic acid) diluted 16% hydrochloric acid (hydrochloric acid) 200 μl. Add 200 μl of DMBA (20 mg / mL) dissolved in acetic acid to each of the prepared mixtures, incubate in a constant temperature water bath at 30°C for 60 minutes, measure the absorbance at 510 nm, and calculate the relative content of the standard substance concentration.

[0055] After measuring the co...

Embodiment 3

[0056] Embodiment 3: HPLC-MS analysis

[0057] According to an embodiment of the present invention, HPLC-MS analysis is performed in order to separate and refine the brown algal polyphenols contained in the above-mentioned final extract (PE).

[0058] Specifically, the PE extracted from Hijiki was analyzed by HPLC (ThermoAccela UPLC, Waltham, MA, USA)-MS (Thermo LTQ-Orbitrap XL, Waltham, MA, USA), the sample was dissolved in distilled water, and the first determination Inject 2 μl at the same time. The column used in the above analysis was ACQUITYBEH C18column (150 2.1mm, 1.7μm), the mobile phase (mobile phase) uses the solvent A that adds 0.1% formic acid to distilled water and the solvent B that adds 0.1% formic acid to acetonitrile (acetonitrile) (HPLC grade, Thermo Fisher Scientific, Waltham, MA, USA) , the solvent gradient initially started with 0% A, 100% B, and after 20 minutes, the concentration of solvent A was increased linearly to reach 80% A, and the analysis wa...

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Abstract

The present invention provides a composition for protecting ultraviolet light, which contains, as an active ingredient, a Sargassum fusiforme extract or a phlorotannin-containing active fraction obtained by separating the Sargassum fusiforme extract through chromatography.

Description

[0001] priority [0002] This application claims priority based on Korean Patent Application No. 10-2015-0056550 filed on April 22, 2015. The above patent application documents are incorporated into this document by reference. technical field [0003] The present invention relates to a composition for ultraviolet shielding, and more specifically relates to a composition for ultraviolet shielding containing hijiki extract as an active ingredient. Background technique [0004] Skin aging is broadly divided into intrinsic aging, which refers to the deterioration of body functions as we age, and extrinsic aging, which refers to aging caused by continuous exposure to ultraviolet rays. (Ultraviolt, UV) and other external stimuli caused by aging. Ultraviolet light is one of the typical causes of photoaging, and it is divided into UV-A (320-400nm), UV-B (280-320nm) and UV-C (100-280nm) according to the length of the wavelength. Most of UV-C is absorbed by the ozone layer, and ver...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9711A61K8/33A61K8/49A61Q17/04A61Q19/08
CPCA61K8/33A61K8/4973A61Q17/04A61Q19/08
Inventor 朱成洙
Owner 人类科学有限公司
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