The invention discloses a novel positive/negative rigidity parallel low-frequency vibration isolation mechanism. The mechanism comprises a positive-rigidity spring adjusting device, a vertical spring body, a negative-rigidity structure, a negative-rigidity spring adjusting device, a damping structure, a bearing structure, a horizontal spring support column and a base support fixed structure, wherein the positive-rigidity spring adjusting device includes a circular sleeve, an adjusting knob and a pressure sensor 1; the negative-rigidity structure includes a horizontal spring body and a sleeve, horizontal spring slide blocks 1 and 2, a horizontal spring connecting rod and a cylinder side wall hinge device; the negative-rigidity spring adjusting device includes a stud and and an adjusting knob thereof, a stud fixed frame, a pressing spring body, a pressing adjusting nut, and a pressure sensor 2 and a fastening nut thereof; the damping structure includes an adjusting cushion plate, a damper and a connecting plate thereof; the bearing structure includes a bearing table and a cylinder side wall; and the base support fixed structure includes a support plate, a disc base, a support cushion leg, a support column and a linear bearing. The mechanism has such vibration isolation advantages as compact structure and accurate adjustment.