Bromelain liposome and preparation method thereof

A technology of bromelain and liposome, which is applied in liposome delivery, peptide/protein components, biochemical equipment and methods, etc., can solve the problems of easy drug leakage, insufficient stability, and poor skin affinity, etc. Achieve the effects of overcoming sensitivity to environmental conditions, improving bioavailability, improving stability and transdermal absorption

Inactive Publication Date: 2016-11-09
AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, traditional liposomes have problems such as insuffic

Method used

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preparation example Construction

[0049] The present invention also provides a kind of preparation method of bromelain liposome, comprises the steps:

[0050] a) dissolving lecithin, cholesterol and Tween-80 in absolute ethanol to obtain a first solution;

[0051] b) The first solution is subjected to magnetic stirring treatment to make the mixed solution into a uniform single phase, and the first solution is placed in a rotary evaporator to evaporate under reduced pressure until a lipid film is formed;

[0052] c) dissolving bromelain in a phosphate buffer solution with a pH value of 6.0-7.0 to obtain a second solution;

[0053] d) adding the second solution to the lipid film to dissolve the lipid film to obtain a third solution;

[0054] e) treating the third solution with a dynamic high-pressure micro-jet with a pressure of 120-130Mpa to obtain bromelain nanoliposomes;

[0055] f) dissolving pectin in a phosphate buffer solution with a pH value of 6.0-7.0 to obtain a fourth solution;

[0056] g) Adding t...

Embodiment 1

[0059] Weigh 100.0g of lecithin and dissolve it in 5L of absolute ethanol, weigh 20.0g of cholesterol and 30.0g of Tween-80 in the aforementioned lecithin solution, place it on a magnetic stirrer and stir for 30min until it is completely dissolved, then place the mixed solution in On a rotary evaporator, rotary evaporate 1H under 40°C water bath condition, remove absolute ethanol to form a layer of lipid film; weigh 30.0g bromelain and dissolve it completely in 10L phosphate buffer solution to obtain bromelain buffer solution, and The homogeneously mixed bromelain buffer solution was added to the lipid film prepared above and rotated to wash the film to obtain a bromelain liposome solution, and then treated twice at 120MPa with a dynamic high-pressure microfluidic device to obtain bromelain nanoliposomes; Weigh 30.0g of apple pectin and dissolve it completely in 5L of phosphate buffer solution, add the apple pectin buffer solution to the bromelain nanoliposomes prepared above, ...

Embodiment 2

[0062] Weigh 500.0g of lecithin and dissolve it in 20L of absolute ethanol, weigh 80.0g of cholesterol and 80.0g of Tween-80 in the aforementioned lecithin solution, place it on a magnetic stirrer and stir for 30min until it is completely dissolved, then place the mixed solution in On a rotary evaporator, rotate evaporating in a water bath at 40°C for 1H to remove absolute ethanol to form a lipid film; weigh 50.0g bromelain and dissolve it completely in 20L phosphate buffer solution to obtain a bromelain buffer solution. The homogeneously mixed bromelain buffer solution was added to the lipid film prepared above and rotated to wash the film to obtain a bromelain liposome solution, and then treated twice at 120MPa with a dynamic high-pressure microfluidic device to obtain bromelain nanoliposomes; Weigh 50.0g of apple pectin and dissolve it completely in 15L of phosphate buffer solution, add the apple pectin buffer solution to the bromelain nanoliposomes prepared above, and stir ...

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Abstract

The invention discloses a bromelain liposome and a preparation method thereof. The bromelain liposome is prepared from the following components by weight: 0.2 to 0.5 percent of bromelain, 1 to 5 percent of lecithin, 0.4 to 0.8 percent of cholesterol, 0.2 to 0.8 percent of Tween 80, and 0.1 to 0.5 percent of pectin. The bromelain liposome provided by the embodiment of the invention can not only improve the transdermal absorption ratio of bromelain and but also increase the stability and the bioavailability of liposome.

Description

technical field [0001] The invention relates to the technical field of protease liposomes, in particular to a bromelain liposome and a preparation method thereof. Background technique [0002] Bromelain is a proteolytic enzyme extracted from pineapple peel and pineapple stem. It is widely used in food, cosmetics, pharmaceuticals and other fields. It has excellent effects such as whitening, whitening and freckle removal. However, because bromelain is sensitive to environmental conditions, it is easy to oxidize and deteriorate, and it is difficult to penetrate the skin to cause defects in application. Liposome is a closed vesicle formed by a phospholipid bilayer and contains an aqueous phase inside. It has the advantages of slow release, cell affinity, and tissue compatibility, and has been successfully used in the fields of medicine, chemical industry, and cosmetics. However, traditional liposomes have problems such as insufficient stability, easy drug leakage, and poor skin...

Claims

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Application Information

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IPC IPC(8): A61K38/48A61K47/36A61K9/127A61K8/73A61K8/66A61K8/14A61Q19/00A23P10/35A23L33/185
CPCA23V2002/00A61K8/14A61K8/66A61K8/73A61K9/1271A61K9/1277A61K38/4873A61K47/36A61K2800/10A61Q19/00C12Y304/22004A23V2200/318A23V2250/548
Inventor 周伟潘琪曹玉坡李积华付调坤
Owner AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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