A Fluorescent Probe for Detecting Hydrogen Sulfide in Cancer Cells
A fluorescent probe, hydrogen sulfide technology, applied in the field of analytical chemistry, can solve the problems of inability to distinguish cancer cells from normal cells, and no cancer cell targeting, achieves good fluorescence emission spectral characteristics, is easy to popularize, and cheap and easy to obtain raw materials Effect
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Embodiment 1
[0029] Preparation of Fluorescent Probe for Detecting Hydrogen Sulfide in Cancer Cells
[0030] Add compound a (1 mmol), sodium azide (1 mmol) and N,N-dimethylformamide (5 mL) represented by formula (II) into a 50 mL one-necked flask, and heat at 90°C for 5 hours. Cool to room temperature, add to water, filter and dry. The resulting solid was purified by column chromatography (dichloromethane:methanol=10:1) to obtain a yellow product, which is a fluorescent probe for detecting hydrogen sulfide in cancer cells according to the present invention, with a yield of 63%.
[0031] The above-mentioned fluorescent probes for detecting hydrogen sulfide in cancer cells 1 H NMR spectrum see figure 1 .
Embodiment 2
[0033] Fluorescence spectra of fluorescent probes at different concentrations of sodium sulfide
[0034] This patent uses sodium sulfide to supply hydrogen sulfide in aqueous solution. Prepare in advance ten 5 mL portions of 5 μM probe buffer solution containing 5% methanol with added sodium sulfide at a concentration of 0 to 150 μM. Fluorescence detection is then performed (λ Ex = 440 nm); calculate the fluorescence intensity in each system; by analyzing the relationship between the fluorescence intensity at 544nm and the concentration of hydrogen sulfide, evaluate the response performance of the probe to sodium sulfide (see figure 2 and image 3 ). figure 2 It shows that with the increase of sodium sulfide concentration, the fluorescence intensity of the solution increases gradually. image 3 It shows that when the concentration of sodium sulfide is in the range of 40 ~ 90 μM, the fluorescence intensity of the solution and the concentration of sodium sulfide are in a g...
Embodiment 3
[0036] Fluorescence spectra of fluorescent probes reacted with different substances
[0037] Prepare 10 parts of 5 mL of 5 μM probe buffer solution (containing 5% methanol, pH = 7.4), and then add 50 μL of 200 μM Hcys, Na 2 S, Na 2 SO 3 , NO, H 2 o 2 , HClO 4 , Cys, GSH, NaNO 2 , VC and other small molecules in PBS solution. Fluorescence detection is then performed (λ Ex = 440 nm); calculate the fluorescence intensity in each system; evaluate the interference of different substances to the fluorescent probe solution (see Figure 4 ). It can be seen from the figure that, of course, adding Hcys and Na to the probe solution 2 S, Na 2 SO 3 , NO, H 2 o 2 , HClO 4 , Cys, GSH, NaNO 2 , VC and other small molecules, only sodium sulfide can cause significant fluorescence in the solution, but the fluorescence of the solution basically does not change when other small molecules are added, which means that the probe is only responsive to sodium sulfide, and not affected by ...
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