Method for lactic acid fermentation thallus residue pretreatment and method for circulation fermentation production of lactic acid

A technology of lactic acid fermentation and cyclic fermentation, applied in fermentation and other directions, can solve the problems of nutrient waste, waste residue pollution, etc., and achieve the effect of reducing dosage, realizing resource recycling, and reducing consumption of raw and auxiliary materials.

Active Publication Date: 2016-11-09
河南金丹乳酸科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] For this reason, the technical problem to be solved by the present invention is to provide a method for pretreatment of lactic acid fermentation cell slag, so as to solve the problems of nutrient waste and waste residue pollution caused by fermentation cell separation in the prior art

Method used

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  • Method for lactic acid fermentation thallus residue pretreatment and method for circulation fermentation production of lactic acid

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Embodiment 1

[0029] The method for cyclic fermentation production of lactic acid described in this embodiment comprises the following steps:

[0030] (1) Raw material preparation: take corn and go through impurity removal, pulverization, pulping, jet liquefaction and filtration; obtain a liquefied sugar solution with a DE value of 22%, and the total sugar content in the liquefied sugar solution is 120g / L;

[0031] (2) Pre-saccharification: The pre-saccharification temperature is 59°C, and the liquefied sugar solution is added into a sterilized and sterilized fermenter for pre-saccharification; 3% hydrochloric acid is added to adjust the pH of the liquefied sugar solution to 4.5±0.1, and the saccharification Add 400U of glucoamylase according to the amount of enzyme added per gram of total sugar in the liquefied sugar liquid, and feed sterile air into the fermenter to keep the pressure in the tank at 0.2Mpa, and the pre-saccharification time is 4 hours to obtain a fermented feed liquid; The...

Embodiment 2

[0039] The method for cyclic fermentation production of lactic acid described in this embodiment comprises the following steps:

[0040] (1) Raw material preparation: the corn is removed from impurities, pulverized, pulped, sprayed and liquefied and filtered; a liquefied sugar solution with a DE value of 28% is obtained, and the total sugar content in the liquefied sugar solution is 180 g / L;

[0041] (2) Pre-saccharification: control the pre-saccharification temperature to 70°C, add the liquefied sugar solution into a sterilized and sterilized fermenter for pre-saccharification; add sulfuric acid with a mass fraction of 2% to adjust the pH of the liquefied sugar solution to 4.4, and glucoamylase According to the amount of glucoamylase added per gram of total sugar in the liquefied sugar liquid, 400 U of glucoamylase was added, and sterile air was introduced into the fermenter to keep the pressure in the tank at 0.1Mpa, and the pre-saccharification time was 6 hours to obtain a f...

Embodiment 3

[0049] The method for cyclic fermentation production of lactic acid described in this embodiment comprises the following steps:

[0050] (1) Raw material preparation: the corn is removed from impurities, pulverized, pulped, sprayed and liquefied and filtered; a liquefied sugar solution with a DE value of 25% is obtained, and the total sugar content in the liquefied sugar solution is 140 g / L;

[0051] (2) Pre-saccharification: The pre-saccharification temperature is 50°C, and the liquefied sugar solution is added into a sterilized and sterilized fermenter for pre-saccharification; hydrochloric acid with a mass fraction of 1% is added to adjust the pH of the liquefied sugar solution to 4.6, and the glucoamylase Add 600 U of glucoamylase per gram of total sugar in the liquefied sugar liquid, and feed sterile air into the fermenter to keep the pressure inside the tank at 0.4Mpa. The pre-saccharification time is 3.5 hours to obtain the fermentation feed liquid. DX value is 90%;

...

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Abstract

The invention belongs to the technical field of lactic acid fermentation, and particularly relates to a method for lactic acid fermentation thallus residue pretreatment and application of the method for circulation fermentation production of lactic acid. The method for pretreatment comprises the steps that thallus residues obtained through filtering and separation of a lactic acid fermentation solution are pretreated through a composite medium; the pretreated thallus residues are filtered and separated to obtain soluble nutrient substances and insoluble impurities; the composite medium is prepared from ammonium sulfate, ethylenediamine tetraacetic acid disodium salt, sodium dodecyl sulfate and sodium citrate. According to the method for lactic acid fermentation thallus residue pretreatment, soluble nutrient substances, including thallus and unconsumed nutrients, obtained after treatment of the composite medium can continue to participate in fermentation, the separated thallus is adopted as a nutrition nitrogen source to be returned to a fermentation working procedure, the consumption of nutrient substances in the fermentation culture medium can be reduced, the problem of fermentation waste residue outlet is solved, consumption of raw and auxiliary materials is lowered, the composite medium can be recycled, and resource recycling is achieved.

Description

technical field [0001] The invention belongs to the technical field of lactic acid fermentation, and in particular relates to a method for pretreatment of lactic acid fermentation bacterium slag and its application for cyclic fermentation to produce lactic acid. Background technique [0002] As one of the three major organic acids (including lactic acid, citric acid, and acetic acid), lactic acid is an organic acid that is made from starch and refined by microbial fermentation. It has a wide range of applications in the fields of food, medicine, and chemical industries. application. [0003] In the field of food industry, due to its gentle and stable acidity, lactic acid is widely used as a sour agent and preservative in the food industry. Therefore, the consumption of lactic acid in the food field accounts for about 60% of the total consumption of lactic acid in the world. Since the human body only has the enzyme to metabolize L-lactic acid, D-lactic acid cannot be absorbe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56
CPCC12P7/56
Inventor 张国宣穆鹏宇师媛媛崔洋崔耀军张树银邢晓霞顾永华张亚辉杨清翔楚慧灵范小辉魏坤罗智慧
Owner 河南金丹乳酸科技股份有限公司
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