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A method for inducing mature embryo callus of wild barley and establishing a regeneration system

A technology for callus induction and establishment method, which is applied in the directions of plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve problems such as imperfection, and achieve the effects of low cost, simple operation and reliable technical support

Active Publication Date: 2018-08-17
LANZHOU UNIVERSITY
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Problems solved by technology

[0004] At present, the research on the in vitro regeneration system of wild barley in China is not perfect. Zhu Jin et al. used mature embryos of wild barley as explants to induce callus, and the salt-tolerant variants screened on MS medium containing NaCl had certain Salt-tolerant and stable, and differentiated into regenerated plants

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  • A method for inducing mature embryo callus of wild barley and establishing a regeneration system
  • A method for inducing mature embryo callus of wild barley and establishing a regeneration system
  • A method for inducing mature embryo callus of wild barley and establishing a regeneration system

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Embodiment 1

[0048] Method concrete steps of the present invention are as follows:

[0049] (1) Selection and treatment of plant materials: Select mature wild barley seeds with uniform color and size, remove the lemma and rinse under running water for 30 minutes, then treat with 75% alcohol for 5 minutes, rinse with sterile water 2-3 times, and use 10 % sodium hypochlorite disinfection for 10 minutes, rinsed with sterile water for 3-5 times, and placed the seeds on sterile filter paper until the seeds are dry and ready for use; the wild barley seeds refer to: wild wild barley collected in Linze, Gansu seed.

[0050] (2) The formulations of induction medium, differentiation proliferation medium and rooting medium are as follows:

[0051] The basal medium is MS medium plus 30g×L sucrose -1 and agar 10 g×L -1 ;

[0052] The callus induction medium is: basal medium + 0.3 mg·L -1 6-BA +1.5 mg·L -1 2,4-D;

[0053] The differentiation and proliferation medium is: basal medium + 0.5mg / L6-...

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Abstract

The invention provides a method for establishing a callus induction and regeneration system for mature embryo of wild barley. The method comprises the following steps: 1) selecting wild barley seeds and disinfecting; 2) selecting an induction medium, a differentiation enrichment medium and a rooting culture medium; 3) treating and inducing differentiation of mature embryo: taking the wild barley seeds disinfected in step 1), putting into the induction medium for culturing, sub-culturing for several times and then transferring into the differentiation and enrichment medium for culturing, sub-culturing for several times and then transferring into the rooting culture medium for culturing, closing the bottle and hardening seedling when the roots of the differentiated seedlings are 4-6cm long, injecting running water after 2d, opening the bottle and hardening seedling for 3d, cleaning the roots, sterilizing and culturing with substrate, thereby acquiring a complete regenerated plant. The callus induction tissue culture method for wild barley, provided by the invention, is simple in operation, low in cost and unlimited by material taking time and space. An established regenerating method and system for mature embryo culture plant of wild barley can provide reliable technical support to the research on the seedling and genetic improvement of cell engineering.

Description

technical field [0001] The invention relates to the field of plant regeneration through tissue culture technology in agricultural cultivation, in particular to a method for inducing callus of mature embryos of wild barley and establishing a regeneration system. Background technique [0002] Wild barley, also known as short-mounted barley, is a perennial herbaceous plant of the Poaceae barley family. It is a perennial herb with short rhizomes and has strong asexual reproduction. It is often distributed in halophyte communities in the form of clusters. In the Songnen Plain The alkalized meadow can form a large area of ​​single dominant community. Wild barley has excellent characteristics such as good grass quality, rich nutrition, high protein and fat content in hay, good palatability, resistance to harsh environments, and trampling resistance. It is the preferred fine variety in the process of artificially cultivated grassland and grassland improvement. [0003] With the deg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王正凤李春杰李秀璋古丽君
Owner LANZHOU UNIVERSITY
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