Preparation method of feed Bacillus subtilis and Chinese medicine bacterial powder

A technology for Bacillus subtilis and forage, which is applied in the field of preparation of Bacillus subtilis traditional Chinese medicine powder for forage, can solve the problems of uneven quality of Bacillus subtilis, unscientific composition of ingredients, backward equipment and technology, etc., and achieves accelerated growth of animals. Speed, broad market application prospects, the effect of promoting rapid growth

Inactive Publication Date: 2016-11-16
河南省岳氏精忠科技有限公司
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  • Description
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Problems solved by technology

At present, there are many Bacillus subtilis production enterprises in the market, but most of them are small-scale enterprises, workshop-style production, backward equipment and technology, uneven quality of Bacillus subtilis, and unscientific ingredients in products. Although some contain a large number of bacteria, they are miscellaneous. High bacteria rate, poor effect

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  • Preparation method of feed Bacillus subtilis and Chinese medicine bacterial powder
  • Preparation method of feed Bacillus subtilis and Chinese medicine bacterial powder

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preparation example Construction

[0018] A preparation method of Bacillus subtilis Chinese medicinal powder for feed includes the following steps:

[0019] Step 1. Inoculate Bacillus subtilis for feed on the slant seed culture medium and cultivate for 48 hours at 35-37°C; the mass percentage content of each component in the slant seed culture medium is: glucose 0.5-1.5%, peptone 0.2-2% , Beef extract 0.5-1.5%, yeast extract 0.5-1.5%, sodium chloride 0.5%, agar 1.5-2%, the balance is water;

[0020] Step 2. Pick 2-4 loops from the freshly cultivated sloping surface and inoculate it in a 250ml Erlenmeyer flask containing 50ml of liquid expansion medium, shake culture at 35-37℃, 180-240rpm shaker for 16-24h to obtain seeds liquid;

[0021] The components and their mass percentages in the liquid expansion medium are: glucose 0.5-1.5%, peptone 0.2-2%, beef extract 0.5-1.5%, yeast extract 0.5-1.5% and sodium chloride 0.5%, the balance Is water

[0022] Step 3. Inoculate the above-mentioned cultured seed liquid into a 5L f...

Embodiment 1

[0031] A preparation method of Bacillus subtilis Chinese medicinal powder for feed includes the following steps:

[0032] Step 1. Inoculate Bacillus subtilis for feed on the slant seed culture medium and culture for 48 hours at 35°C; the mass percentage content of each component in the slant seed culture medium is: glucose 0.5%, peptone 0.2%, beef extract 0.5%, Yeast extract 0.5%, sodium chloride 0.5%, agar 1.5%, the balance is water;

[0033] Step 2. Pick 2 loops of lawn from the freshly cultivated slope, inoculate it in a 250ml Erlenmeyer flask containing 50ml of liquid expansion medium, and culture at 35°C with a shaker at 180rpm for 24h to obtain seed liquid;

[0034] The components and their mass percentage contents in the liquid expansion medium are: glucose 0.5%, peptone 0.2%, beef extract 0.5%, yeast extract 0.5% and sodium chloride 0.5%, the balance being water;

[0035] Step 3. Inoculate the above-mentioned cultured seed liquid into a 5L fermentor equipped with 3L culture li...

Embodiment 2

[0045] A preparation method of Bacillus subtilis Chinese medicinal powder for feed includes the following steps:

[0046] Step 1. Inoculate Bacillus subtilis for feed on the slant seed culture medium and culture for 48 hours at 37°C; the mass percentage content of each component in the slant seed culture medium is: glucose 1.5%, peptone 2%, beef extract 1.5%, Yeast extract 1.5%, sodium chloride 0.5%, agar 2%, the balance is water;

[0047] Step 2: Pick 4 loops of lawn from the freshly cultivated slope, inoculate it in a 250ml Erlenmeyer flask containing 50ml of liquid expansion medium, and cultivate it with shaking at 37°C and 240rpm for 16 hours to obtain seed liquid;

[0048] The content of each component and its mass percentage in the liquid expansion medium are: glucose 1.5%, peptone 2%, beef extract 1.5%, yeast extract 1.5% and sodium chloride 0.5%, the balance being water;

[0049] Step 3. Inoculate the above-mentioned cultured seed liquid into a 5L fermentor equipped with 3L cu...

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Abstract

The present invention relates to a preparation method of feed Bacillus subtilis and Chinese medicine bacterial powder, including: activating feed Bacillus subtilis, and inoculating to a seed culture solution for culturing to obtain a seed solution; inoculating the seed solution to a culture solution for culturing to obtain a secondary seed solution; inoculating the secondary seed solution to a liquid fermentation medium, and culturing to obtain a fermentation broth; taking Rhizoma Rehmanniae, Pericarpium Citri Reticulatae, Rhizoma Atractylodis Macrocephalae, Radix Ophiopogonis, Radix Trichosanthis, Fructus Crataegi and medical stone powder, crushing, adding water for soaking, decocting and filtering, and concentrating filtrate to obtain concentrate; mixing well the fermentation broth and the concentrate according to the volume ratio of (8-10): (1-3) to obtain mixture, conveying the mixture to a double-effect vacuum concentrating apparatus by using a pump, concentrating, adding starch, mixing well, atomizing, performing concurrent drying with hot air, and recycling to obtain the feed Bacillus subtilis and Chinese medicine bacterial powder; The preparation method of the bacterial powder is less than 6% in water content, has a viable count of 8000-18000 hundred million / g, and can keep 95% of strain activity within two years.

Description

Technical field [0001] The invention relates to a preparation method of bacterial powder, in particular to a preparation method of traditional Chinese medicine bacterial powder of Bacillus subtilis for feed. Background technique [0002] Bacillus subtilis is a commonly used bacteria in microbial preparations. After entering the animal’s intestinal tract in the form of spores, Bacillus subtilis can quickly re-germinate from a dormant state. As an aerobic bacteria, Bacillus subtilis can quickly re-germinate during germination and growth. The free oxygen in the digestive tract is consumed quickly and in large quantities, resulting in an anoxic environment; the harmful bacteria are generally aerobic bacteria, so their growth is inhibited, and the anaerobic beneficial flora becomes the dominant flora. At the same time, Bacillus subtilis also has strong protease, amylase and lipase activities, which can activate proenzymes in the body into active enzymes, and can also secrete a series ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/30A23K10/18C12N1/20C12R1/23
CPCC12N1/20Y02P60/87
Inventor 岳占州王新昌安建军杨雪莲岳朝鹏张亚飞
Owner 河南省岳氏精忠科技有限公司
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