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Skin regeneration material and preparation method thereof

A technology of skin regeneration and pigskin, which is applied in the field of medical materials, can solve problems such as insufficient skin donor sites, and achieve the effects of shortening healing time, good cell compatibility, and beneficial regeneration

Inactive Publication Date: 2016-11-16
林春梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional skin repair materials include dressings, heterogeneous skin grafts, allogeneic skin grafts and autologous skin grafts, etc., but many times there are often insufficient skin donor sites, and multiple transplants are required to cure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A skin regeneration material, prepared from the following ingredients in parts by weight: 20 parts of pig skin collagen, 20 parts of tilapia skin collagen, 5 parts of O-carboxymethyl chitosan, 0.1 part of Scutellaria baicalensis extract, ascorbyl palmitate 0.2 parts of acid ester, 3 parts of silicone adhesive, 0.1 parts of citric acid, 0.2 parts of glutaraldehyde, 0.1 parts of potassium sorbate, 40 parts of acetic acid, 30 parts of concentrated sulfuric acid, 400 parts of absolute ethanol, and 120 parts of deionized water.

[0027] The preparation method of the above skin regeneration material is as follows: (1) mix O-carboxymethyl chitosan and concentrated sulfuric acid, put it in an ice-water bath and stir for 30 minutes at a speed of 800r / min; (2) add 100 parts of deionized water , and continue to stir for 5 minutes; (3) add 300 parts of absolute ethanol for precipitation; (4) filter, put it into a dialysis bag and freeze-dry at a temperature of -20°C; , citric acid,...

Embodiment 2

[0029] A skin regeneration material, prepared from the following ingredients in parts by weight: 22 parts of pig skin collagen, 22 parts of tilapia skin collagen, 6 parts of O-carboxymethyl chitosan, 0.12 parts of Scutellaria baicalensis extract, ascorbyl palmitate 0.3 parts of acid ester, 4 parts of silicone adhesive, 0.15 parts of citric acid, 0.3 parts of glutaraldehyde, 0.11 parts of potassium sorbate, 45 parts of acetic acid, 32 parts of concentrated sulfuric acid, 420 parts of absolute ethanol, and 130 parts of deionized water.

[0030] The preparation method of the above skin regeneration material is as follows: (1) mix O-carboxymethyl chitosan and concentrated sulfuric acid, put it in an ice-water bath and stir for 35 minutes at a speed of 850r / min; (2) add 110 parts of deionized water , continue to stir for 6 minutes; (3) add 320 parts of absolute ethanol for precipitation; (4) filter, put it into a dialysis bag and freeze-dry at a temperature of -19°C; , citric acid,...

Embodiment 3

[0032] A skin regeneration material, prepared from the following ingredients in parts by weight: 25 parts of pig skin collagen, 25 parts of tilapia skin collagen, 7.5 parts of O-carboxymethyl chitosan, 0.15 parts of Scutellaria baicalensis extract, ascorbyl palmitate 0.35 parts of acid ester, 4.5 parts of silicone adhesive, 0.2 parts of citric acid, 0.35 parts of glutaraldehyde, 0.15 parts of potassium sorbate, 50 parts of acetic acid, 35 parts of concentrated sulfuric acid, 450 parts of absolute ethanol, and 150 parts of deionized water.

[0033] The preparation method of the above skin regeneration material is as follows: (1) mix O-carboxymethyl chitosan and concentrated sulfuric acid, put it in an ice-water bath and stir for 35 minutes at a speed of 850r / min; (2) add 125 parts of deionized water , and continue to stir for 7 minutes; (3) add 350 parts of absolute ethanol for precipitation; (4) filter, put it into a dialysis bag and freeze-dry at a temperature of -17°C; , cit...

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PUM

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Abstract

The invention provides a skin regeneration material and a preparation method thereof. The preparation method comprises the following steps that O-carboxymethyl chitosan and concentrated sulfuric acid are mixed and stirred, deionized water is added, stirring is carried out, absolute ethyl alcohol is added for sediment, filtering and freeze-drying are carried out, and then the product is mixed with radix scutellariae extract, 6-O-palmitoyl-L-ascorbic acid, citric acid, potassium sorbate, acetic acid and deionized water; pigskin collagen, tilapia skin collagen, acetic acid and deionized water are mixed to be uniform; the two solutions are mixed and stirred, after being subjected to vacuum defoamation, the mixture is injected into a culture plate to be frozen dry and then treated through glutaraldehyde and absolute ethyl alcohol mixed liquid, and after rinsing, a porous scaffold is obtained; finally, the surface of a medical silicone rubber film is coated with an organic silica gel adhesive, and the medical silicone rubber film and the porous scaffold are combined to obtain the skin regeneration material. The skin regeneration material has good cytocompatibility, no obvious cytotoxicity appears, and the skin regeneration material has good vascularization ability and can effectively promote wound healing and carry out wound repairing.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a skin regeneration material and a preparation method thereof. Background technique [0002] Skin is the largest organ of the human body, composed of epidermis, dermis and subcutaneous tissue, and contains accessory organs, blood vessels, lymphatic vessels and nerves. The skin can prevent the loss of water and electrolytes in the body, protect the body from radiation in the external environment and the direct harm of chemicals, bacteria and viruses, and at the same time can regulate the balance of water and other substances in the body, and has the function of regulating body temperature and sensing external information. effect. However, a large area of ​​skin tissue exposed to the external environment may be damaged at any time. Burns, mechanical trauma, and ulcers caused by chronic diseases are the main reasons for skin defects and loss of function, and a large number of patie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/54A61L27/36A61L27/24A61L27/20
CPCA61L27/60A61L27/20A61L27/24A61L27/3637A61L27/54A61L2300/412C08L89/00C08L5/08
Inventor 林春梅
Owner 林春梅