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Method for determining detergent fiber content in straw residues of sweet sorghum

A technology for sweet sorghum straw and fiber washing, applied in the agricultural field, can solve problems such as cumbersome experimental steps, and achieve the effects of accurate and reliable results and simple operation steps.

Inactive Publication Date: 2016-11-16
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this technology is that it is necessary to use a pressure vessel (pressure cooker) for the experiment, and the experimental steps are cumbersome
The disadvantage of this technology is that it needs to use precision instruments such as high-phase liquid chromatography (HPLC) and ultraviolet spectrophotometer (UV) to carry out experiments, and the experimental steps are relatively cumbersome.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. Pretreatment of sweet sorghum stalks: remove the roots and leaves after harvesting the sweet sorghum stalks, and dry them;

[0056] After the sweet sorghum stalks are harvested, the roots and leaves are removed, and the juice and straw biomass of the sweet sorghum are obtained by squeezing through a juicer. The straw biomass is air-dried, ground and sieved to obtain a 40-60 mesh biomass sample. Then the stalks were washed three times according to the ratio of distilled water to sweet sorghum stalks of 10:1 to remove residual sucrose, glucose, fructose and other substances on the stalk biomass. Then dry it in an oven at 60°C for 24 hours, cool it to room temperature in a desiccator, and seal it with a sealed bag to avoid the degradation of the biomass by substances such as cellulase and cause errors in the experiment.

[0057] 2. Configure neutral detergent

[0058] The neutral detergent adopts 25-35g / L sodium lauryl sulfate solution, preferably 30g / L sodium lauryl ...

Embodiment 2

[0076] 1. Pretreatment of sweet sorghum stalks, obtaining sweet sorghum stalk residues after pretreatment;

[0077] After the sweet sorghum stalks are harvested, the roots and leaves are removed, and the juice and straw biomass of the sweet sorghum are obtained by squeezing through a juicer. The straw biomass is air-dried, ground and sieved to obtain a 40-60 mesh biomass sample. Then the stalks were washed three times according to the ratio of distilled water to sweet sorghum stalks of 10:1 to remove residual sucrose, glucose, fructose and other substances on the stalk biomass. Then dry in an oven at 60°C for 24 hours, cool to room temperature in a desiccator, and seal with a sealed bag to avoid cellulase and other substances from degrading the biomass and causing errors in the experiment.

[0078] 2. The configuration method of neutral detergent (30g / L sodium lauryl sulfate) is as follows: Take 100ml beaker and weigh 18.61g disodium edetate (Na 2 EDTA), 6.81g sodium tetrabo...

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PUM

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Abstract

The invention provides a method for determining the detergent fiber content in the straw residues of sweet sorghum. The method includes: pretreating sweet sorghum straw; preparing neutral detergent, acid detergent and sulfuric acid; digesting a pretreated sample with the neutral detergent, and calculating to obtain the content of neutral detergent fibers; reweighing a sample, digesting the sample with the acid detergent, and calculating to obtain the content of acid detergent fibers; treating with the sulfuric acid, and calculating to obtain the content of acid detergent lignin, cellulose content, hemicellulose content and lignin content. The method is simple in procedure, capable of conveniently and fast determining various parameters such as the content of the neutral detergent fibers in the straw residues of the sweet sorghum, and capable of providing data and theoretical support for the resource utilization of the straw residues of the sweet sorghum.

Description

technical field [0001] The invention relates to a method for measuring substance content in sweet sorghum stalks, in particular to a method for measuring washing fiber content in sweet sorghum stalk residues, which belongs to the field of agricultural technology. Background technique [0002] In agricultural production, a large amount of biomass waste will be produced, and the resource and energy utilization of these wastes has been a research hotspot for a long time. Sweet sorghum is a renewable green energy crop. Most studies focus on the use of sweet sorghum stalk juice to ferment ethanol, but its stalk residue is also a resource that can be further developed and utilized. [0003] Through literature search of the prior art, it is found that the Chinese patent application number is CN 201110091984.5, the publication number is CN 102735566A, and the name is: Determination method of acid detergent fiber content in forage grass. The forage grass and the acid washing solutio...

Claims

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Application Information

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IPC IPC(8): G01N5/04G01N1/28
CPCG01N1/28G01N5/04
Inventor 刘荣厚蔡文飞赫倚风刘彪彪崔少峰
Owner SHANGHAI JIAO TONG UNIV
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