Method for extracting fatty acid through waste grease enzyme method
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A technology of waste oil and enzymatic extraction, applied in the field of fatty acid extraction, can solve problems such as environmental pollution and impact on food safety.
Inactive Publication Date: 2016-11-23
淮南市蓝科火炬生物科技有限公司
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Problems solved by technology
Conversely, waste cooking oil has become an environmental pollutant and impacts food safety
Method used
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Embodiment 1
[0027] A method for enzymatically extracting fatty acids from waste oil, comprising the steps of:
[0028] (1) First, heat and stir the waste oil (removal of impurities), use activated clay as the decolorizing and deodorizing agent, and decolorize under normal pressure. While decolorizing the waste oil, it can also remove odor, some metal ions, soap particles, glue to meet the quality requirements of enzymatic hydrolysis for waste oil; the decolorization and deodorization process is carried out under heating, stirring and stirring conditions, the temperature is 50-130°C, the stirring speed is 60-200r / pm, and the contact time is 0.1-200r / pm. 0.5h, the dosage of the filter medium activated clay is 1%-6% of the total amount of waste oil. ;
[0029] (2) Wash the decolorized and deodorized oil with water to remove water and impurities to obtain neutral purified oil;
[0030] (3) Mix the neutral purified oil and compound lipase for hydrolysis reaction to obtain a hydrolysis mixed ...
Embodiment 2
[0035]A method for extracting fatty acids by enzymatic method of waste oil, wherein the compound enzyme is a compound enzyme composed of Candida lipolytica lipase and lipase "Amano" 50 produced by filamentous bacteria of the genus Penicillium, the compound enzyme Among them, "Amano" 50 lipase accounts for 10-30%, Candida lipolytica lipase accounts for 70-90%; the enzyme dosage is 2500-25000 U / 100g oil. The oil hydrolysis is carried out at a temperature of 15-50° C., normal pressure, a pH value of 5-9, and a shaker speed of 150-220 rpm. The hydrolysis time is 15-26 hours, and the weight ratio of waste oil and water is 1:0.5-1:5; the others are the same as in Example 1.
Embodiment 3
[0037] A method for enzymatically extracting fatty acids from waste oil, the method also includes recovering glycerin, and the others are the same as in Example 1.
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Abstract
The invention discloses a method for extracting fatty acid through a waste grease enzyme method. The method includes the following steps that firstly, waste grease without impurities is heated and stirred, activated white clay serves as a decoloring and deodorizing agent, normal-pressure decoloring is adopted, when the waste grease is decolorized, odor, part of metal ions, soap grains, colloid and impurities can be removed, and quality requirements of the waste grease for enzymolysis are met; secondly, the decolorized and deodorized grease is washed with water, water and impurities are removed, and neutral purified grease is obtained; thirdly, the neutral purified grease and composite lipase are mixed for hydrolysis reaction, and a hydrolysis mixed product is obtained; fourthly, the hydrolysis mixed product is separated to obtain upper-layer oil phase substances in a separation tank, distillation is continuously conducted after water washing to obtain mixed fatty acid, and the product is obtained. The method has the advantages of being low in energy consumption, little in equipment investment, simple in process, easy and convenient to operate, low in processing cost and the like, is suitable for production on different scales, facilitates resource development and utilization of kitchen waste grease, reduces environment pollution and improves work conditions.
Description
technical field [0001] The invention relates to a method for extracting fatty acid, in particular to a method for enzymatically extracting fatty acid from waste oil. Background technique [0002] Waste oil can be divided into three categories: one is gutter oil (swill oil, swill oil, etc.), that is, the greasy floating matter in the sewer or the oil that is simply processed and extracted from the leftovers and leftovers of hotels and restaurants; The oil produced after the processing and refining of inferior animal viscera or meat; the third is the oil used for frying food that has been used more than the specified number of times, and then reused or re-used after adding some new oil to it. Long-term consumption of waste oil is extremely harmful to the human body. The heavy metals and toxins (such as acrolein) in waste oil seriously exceed the standard, and the peroxide value is higher than 0.4%, far exceeding the national standard of 0.15%. Long-term intake will cause cell ...
Claims
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