Effective separation method of Ustilaginoidea virens (Cooke) Takahashi capable of producing five ustiloxins
A technology of rice smut toxin and Chlorocytosis oryzae, which is applied in the field of effective separation of Chrysanthemum oryzae, can solve problems affecting the germination, growth and colony purification of chlamydospores, increase the difficulty of separation and purification, and limit environmental conditions, etc. question
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0013] The present invention a kind of effective separation method of the sclerotinia oryzae that produces five kinds of rice smut toxins, 1, the collection of rice bent ball sample: the fresh single-grain yellow, yellow-green or green rice bent ball collected from the field.
[0014] 2. Preparatory work for bacterial strain isolation: preparation of 75% alcohol; sterile filter paper and sterile scalpel; filter-sterilized 100g / L chloramphenicol mother liquor (prepared by absolute ethanol).
[0015] 3. Isolation of bacterial strains: Soak the collected rice koji ball disease grains in 75% alcohol for 10 seconds, then put them on an alcohol lamp to ignite spontaneously for about 5 seconds, dry the surface moisture with sterile filter paper, and remove the surface tissue Clean up under aseptic conditions. Then with a sterile scalpel the rice ball is cut open, the middle layer is clamped, and it is inoculated to a concentration of 0.05g / L chloramphenicol (inhibiting bacterial grow...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com