Boletus culture medium with addition of bacterial nano cellulose and preparation method for liquid bacterial strain of boletus

Abstract

The invention discloses a boletus culture medium with addition of bacterial nano cellulose, which includes, by weight, 98-100 parts of porous corn starch, 89-90 parts of yeast extract powder, 36-38 parts of glucose, 2.9-3 parts of edible peanut oil, 1.5-1.6 parts of sodium bicarbonate, 2.1-2.2 parts of egg yolk, 2.2-2.3 parts of polyaspartic acid, 1.9-2 parts of oil of camellia assamica, 1.4-1.5 parts of ethyl maltol, 4.6-4.8 parts of nano-scale tourmaline powder, 6.8-7 parts of bacterial nano cellulose, and the like. The porous corn starch and the yeast extract powder are employed with nutritional additives to prepare the liquid bacterial strain of boletus, which is rich in nutrition. The bacterial nano cellulose modifies the porous corn starch, so that on one hand, the raw material components of the culture medium are increased; and on the other hand, the culture medium is improved in uniformity, stability and space structure characters, thereby providing guarantee for excellent generation and growth and development of mycelium pellets of the liquid bacterial strain of boletus.

Description

technical field [0001] The invention relates to the technical field of liquid strains, in particular to a boletus culture medium added with bacterial nanocellulose and a preparation method for boletus liquid strains. Background technique [0002] In the production process of boletus, liquid strains have incomparable advantages over solid strains. Liquid strains have short production cycle, short and consistent bacterial age, high purity, strong vitality, simple and fast inoculation, and easy implementation of industrialization and large-scale production. The advantages of modernization and standardized production. Now, more and more people are researching and using liquid strains, and the promotion and use of liquid strains is also an inevitable trend to realize industrialization, scale and standardization of edible fungi. However, liquid strains also have their own defects and deficiencies: 1. The germination point of liquid strains usually grows quickly, but the penetrati...

AI Technical Summary

Problems solved by technology

However, liquid strains also have their own defects and deficiencies: 1. The germination point of liquid strains usually grows quickly, but the penetrating power is not strong. If the compost is too thick, mycelium is not easy to penetrate the compost, and the mycelia will follow the compost. The surface grows rapidly, and the amount of mycelium inside the compost is very small, which seriously affects the yield and quality of the strain; 2. Although the conventional liquid strain solves some problems existing in the solid strain, it can significantly shorten the cultivation cycle and enhance the growth rate of the strain. Species vigor and stress resistance, reducing the contamination rate of strains, and improving the uniformity of strain quality, but there are still some deficiencies such as the density, size, and uniformity of the bacterial balls in the liquid strains obtained by fermentation are not very ideal. If the bacteria balls are crushed by stirring and shearing, the uniformity of the obtained strains will be more ideal. However, the shear force of high-speed stirring, crushing and beating will greatly damage the strains and have a serious impact on the overall quality of the strains.

Publication No. CN105309194A "New Technology for the Production of Edible Mushroom Microparticle Liquid Strain" provides a kind of edible fungus microparticle liquid strain with a mycelium ball particle diameter of 0.5mm, which increases the ventilation rate of the edible fungus liquid strain culture solution, so that the ventilation rate It is suitable at 1:0.6—0.9V / V·min. At the same time, a stirrer is installed in the aerated fermenter to make the eddy current of the culture liquid move and prolong the technical operation method of the air bubble. The method for producing the microparticle liquid strain is from the production process There is no improvement in the aspect of liquid culture medium, and the implementation is technically difficult and demanding; Publication No. CN105130516A "Method for Making Molecular-level Biological Medium" mentions that the nutrients of molecular-level biological medium can be absorbed quickly and comprehensively. Promotes the growth of liquid strains, but does not mention the effect of molecular-grade biological media on the diameter of liquid strain mycelium balls