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A Saprolegnia antagonistic actinomycetes qhv2, isolation and identification method and application

A technology of actinomycetes and actinomycetes, applied in the direction of antifungal agents, isolation of microorganisms, methods based on microorganisms, etc., to achieve the effect of stable antibacterial activity

Active Publication Date: 2019-08-02
沅陵县鱼翔生态农业开发有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the application of actinomycetes in the control of saprolegniasis. In this study, saprolegniasis antagonistic actinomycetes with stable antibacterial activity were isolated and screened from the sediment of healthy pearl culture water, and their morphology and molecular biology were identified. In order to provide some technical support for the research and development of biological control drugs for saprolegniasis

Method used

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  • A Saprolegnia antagonistic actinomycetes qhv2, isolation and identification method and application
  • A Saprolegnia antagonistic actinomycetes qhv2, isolation and identification method and application
  • A Saprolegnia antagonistic actinomycetes qhv2, isolation and identification method and application

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Embodiment 1

[0030] (1) Collection and processing of mud samples: The mud samples were collected from the lake bottom mud of Hunan Miluo and Qianshan Red Pearl Mussel Healthy Breeding Bases. The collected mud samples were naturally air-dried at room temperature, and then the air-dried mud samples were dried with a mortar. Grind and set aside.

[0031] (2) Culture medium preparation

[0032] Gaoshi No. 1 medium: the ingredients include soluble starch 2g, KNO 3 0.1g, K 2 HPO 4 0.05g, MgSO 4 · 7H 2 O0.05g, NaCl 0.05g, FeSO 4 · 7H 2 O 0.001g, agar 1.8g, first use 10-20 mL of cold water to make starch into a paste, pour into 60-70 mL of boiling water, heat and stir until transparent, after cooling, add other ingredients in the medium formula except starch After dissolving, adjust the volume to 100 mL, adjust the pH to 7.4-7.6, and sterilize at 121°C for 20 minutes for the purification and cultivation of actinomycetes.

[0033] Gaoshi No. 1 double-antibody medium: add nalidixic acid (fi...

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Abstract

The invention discloses a strain of saprolegnia antagonizing actinomycetes QHV2, a separation and identification method and application. This strain of Saprolegnia antagonistic actinomycetes QHV2 belongs to the genus Streptomyces and is classified as Streptomyces hillocks Streptomyces collinus , the deposit number is CCTCC NO: M2016140, which was deposited in the China Center for Type Culture Collection on March 23, 2016, and the deposit address is Wuhan University, Wuhan City, Hubei Province, China. The antibacterial activity test showed that the growth of saprolegnia was significantly inhibited after 3-5 days of continuous growth, and the fermentation was carried out for 5-7 days, which showed good anti-sprouting activity. The stability test showed that after 10 generations of continuous passage, QHV2 within 3-5 days could significantly inhibit the growth of Saprolegnia hyphae. The actinomycete QHV2 of the present invention has obvious antagonistic effect on the saprolegnia pathogen, and provides a certain reference basis for the prevention and control of saprolegnia.

Description

technical field [0001] The invention relates to a Saprolegnia antagonizing actinomycete QHV2 and its isolation and identification method, as well as its application. Background technique [0002] Saprolegnia ( Saprolegnia ) is a parasitic disease Saprolegniasis ( Saprolegniasis ) of the main pathogenic bacteria. Saprolegnia has no strict selectivity to hosts, and is a worldwide disease that seriously threatens freshwater farmed fish. Especially in the late hatching period of sticky eggs, Saprolegnia can even infect fertilized and developed fish eggs, and the hyphae entanglement will suffocate the fertilized eggs to death. . In the production practice of saprolegniasis prevention and control, a variety of drugs have been used for prevention and control, such as salicylic acid, "galacha", malachite green, etc. Among them, the most effective drug is malachite green, but because of its high residue and " The "three causes" (carcinogenic, teratogenic, and sudden) effects hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/689C12N1/02A61K35/74A61P31/10C12R1/465
CPCA61K35/74C12N1/02C12Q1/689C12N1/205C12R2001/465
Inventor 罗玉双杨品红王文彬唐琳李娜陈飘李德斌
Owner 沅陵县鱼翔生态农业开发有限责任公司