Gastric cancer genetic susceptible gene screening reagent kit

Abstract

The invention discloses a gastric cancer genetic susceptible gene screening reagent kit. The gastric cancer genetic susceptible gene screening reagent kit comprises specific primers of rs1801133 loci of MTHFR (methylenetrahydrofolate reductase) genes, rs2031920 loci of CYP2E1 (cytochrome P450 2E1), rs25478 loci of XRCC1 (X-ray repair cross-complementing group 1) genes and res1136410 loci of ADPRT [ADP (adenosine diphosphate)-ribosyltransferase] genes and corresponding extension probes. The gastric cancer genetic susceptible gene screening reagent kit has the advantages that the rs1801133 loci of the multiple MTHFR genes, the rs2031920 loci of the CYP2E1, the rs25478 loci of the XRCC1 genes and the res1136410 loci of the ADPRT genes can be simultaneously detected by the gastric cancer genetic susceptible gene screening reagent kit, and the gastric cancer genetic susceptible gene screening reagent kit is low in detection cost; a plurality of samples can be simultaneously detected at one step, and accordingly the gastric cancer genetic susceptible gene screening reagent kit is high in throughput.

Description

technical field [0001] The invention relates to the fields of molecular biology and medicine, in particular to a screening kit for gastric cancer hereditary susceptibility genes. Background technique [0002] Gastric cancer is one of the most common malignant tumors of the digestive tract, and is a common and frequently-occurring disease that seriously threatens human health and life. According to statistics, about 900,000 people in the world are newly diagnosed with gastric cancer every year. In my country, gastric cancer ranks first among digestive tract malignancies and third among systemic malignancies. According to the data released by the Health Information Statistics Center of the Ministry of Health in 2001, malignant tumors are the first cause of death among urban residents in my country, with a mortality rate of 135.59 / 100,000 (24.9%). Among them, gastric cancer is the third leading cause of death from malignant tumors. The occurrence and development of gastric ca...

AI Technical Summary

Problems solved by technology

The rs25487 polymorphic site of XRCC1 is just located in the BRCT-domain that interacts with ADPRT, so this gene-gene interaction has a biological basis, and the rs1136410 polymorphic site of ADPRT and the rs25487 polymorphic site variation of XRCC1 have Interaction, the simultaneous mutation of the two reduces the function of base excision repair and affects the susceptibility of individuals to cancer

[0007] The existing technology detects gastric cancer susceptible populations, using hybridization chip method or glass plate chip method, and using taqman probes. This method has low accuracy, high false negative and false positive rates, and cannot be detected in batches; another direct sequencing Although the accuracy rate is high, the cost is high and batch detection cannot be realized. Therefore, none of the existing methods can meet the requirements of large-scale genetic screening.