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Preparation method of electrochemical sensor for detecting polymorphism of ADRB1-115G>C gene

A polymorphism detection, C gene technology, applied in scientific instruments, instruments, measuring devices, etc., can solve the problem of not being able to be used as redox electrical equivalents, to improve sensitivity and biocompatibility, increase immobilization capacity, promote effect of development

Active Publication Date: 2017-01-04
CHONGQING MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, nucleic acid molecules cannot be used as redox couples in biological assay reactions due to their inert nature.

Method used

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  • Preparation method of electrochemical sensor for detecting polymorphism of ADRB1-115G>C gene
  • Preparation method of electrochemical sensor for detecting polymorphism of ADRB1-115G>C gene
  • Preparation method of electrochemical sensor for detecting polymorphism of ADRB1-115G>C gene

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Embodiment 1

[0045] Step 1. Disperse 10mg GO in 5mL ultrapure water to form a suspension, followed by 10mL, 0.025M Ce(NO 3 ) 3 ·6H 2 O was mixed with 10mL, 0.025M urotropine (HTMA), and then the above two mixed solutions were placed in a water bath at 80°C for 5 hours, and the resulting solution was centrifuged at 10,000r / min for 5 minutes, and washed with ultrapure water and ethanol. Wash 3 times respectively, and dry the obtained precipitate in a constant temperature drying oven at 60°C under reduced pressure, and set aside;

[0046] Step 2. 20mg GO-CeO 2 Disperse in 5mL absolute ethanol, then add 0.1mLAPTES, and heat at reflux at 70°C for 1.5h. After the above solution was cooled to room temperature, it was centrifuged at 8000r / min for 5 minutes, and washed 3 times with ultrapure water. The obtained precipitate was dried in a constant temperature drying oven at 50°C for 12 hours, and set aside;

[0047] Step 3. 1mL H 2 PtCl 6 (1%) add 1mL, 2mg mL -1 GO-CeO 2 solution, sonicated...

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Abstract

The invention relates to a preparation method and an application of an electrochemical sensor for detecting the polymorphism of metoprolol personalized medicine gene-beta1 adrenergic receptor (ADRB1), and belongs to the technical field of electrochemical detection. The preparation method is characterized by comprising the following steps: firstly reducing cerium dioxide (CeO2) onto graphene oxide (GO) to obtain a GO-CeO2 composite material, reducing platinum nano particles on the surface of the composite material, and mixing a single-chain DNA probe with the composite material to obtain a detection probe; self-assembling nano gold and avidin to fix the biotinylation single-chain DNA capturing probe, thereby preparing the electrochemical sensor for detecting the polymorphism of the ADRB1-1165G>C gene. The sensor is successfully used for detecting the single base mutation of the ADRB1 gene. The electrochemical sensor for detecting the polymorphism of the ADRB1-1165G>C gene has the advantages of high sensitivity, high specificity, and rapidness and convenience in detection. A novel detection method is provided for the metoprolol personalized medicine.

Description

[0001] Technical field: [0002] The invention relates to a preparation method and application of an electrochemical sensor for the detection of ADRB1-1165G>C gene polymorphism for clinically guiding warfarin medication, especially a sandwich prepared based on ceria nanocomposite materials as signal probes A type biosensor is used for detecting ADRB1-1165G>C gene polymorphism, and belongs to the field of electrochemical detection. [0003] Background technique: [0004] As a commonly used β-receptor blocker, metoprolol is commonly used in the treatment of hypertension, angina pectoris, myocardial infarction, arrhythmia, and cardiac neurosis. However, the predicted dose of metoprolol for each patient may be over or under due to the lack of individual genetic information of the patient. Therefore, it is necessary to precisely adjust the dosage of metoprolol according to individual differences. This difference in drug use is determined by the target site of metoprolol and ...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 于超赵一璘陈俊吴静
Owner CHONGQING MEDICAL UNIVERSITY
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