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A kind of method for extracting avelamycin

A technology of avilamycin and alkaline solvent, applied in the field of extracting avilamycin, can solve the problems of long process, low product yield and high cost, and achieve the effects of less process, lower ammonia nitrogen content and short working hours

Active Publication Date: 2020-10-30
HEBEI SHENGXUE DACHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a method for extracting avelamycin with low cost, short operation and high yield, which solves the problem of high cost and long operation of the avelamycin extraction method in the prior art, resulting in The problem of low product yield

Method used

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  • A kind of method for extracting avelamycin

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Experimental program
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Effect test

Embodiment 1

[0027] With 50L of avelamycin fermented liquid, titer 4656U / mL, product weight 233g. Use sodium hydroxide to adjust the pH to 8.2, followed by solid-liquid separation; the obtained solid is dried to obtain a dry powder and then pulverized, an organic solvent is added to the pulverized solid, and stirred for 1.5 hours, during which the temperature is controlled at 42 ° C, filtered, and the obtained filtrate Standby; the filtrate is decolorized with 2% activated carbon, and the obtained solution is transferred to a non-polar macroporous adsorption resin for adsorption, and then the resin column is analyzed with 60% ethanol to collect the analysis solution; the analysis solution is evaporated, concentrated and dried to obtain Avila Mycin finished product 128g, content 82.1%, yield 54.9%.

Embodiment 2

[0029] With neomycin sulfate fermentation liquid 60L, potency 4832U / ml, product weight 290g. Use sodium hydroxide to adjust the pH to 8.5, followed by solid-liquid separation; the obtained solid is dried to obtain a dry powder and then pulverized, an organic solvent is added to the pulverized solid, stirred for 2.0 hours, during which the temperature is controlled at 45 ° C, filtered, and the obtained filtrate Standby; the filtrate is decolorized with 3% activated carbon, and the obtained solution is transferred to a non-polar macroporous adsorption resin for adsorption, and then the resin column is analyzed with 65% ethanol to collect the analysis solution; the analysis solution is evaporated, concentrated and dried to obtain Avila Mymycin finished product 165g, content 81.5%, yield 56.9%.

Embodiment 3

[0031] With 70L of neomycin sulfate fermented liquid, titer 4938U / mL, product weight 346g. Use sodium hydroxide to adjust the pH to 8.7, followed by solid-liquid separation; the obtained solid is dried to obtain a dry powder and then pulverized, an organic solvent is added to the pulverized solid, stirred for 2.5 hours, during which the temperature is controlled at 48 ° C, filtered, and the obtained filtrate Standby; the filtrate is decolorized with 5% activated carbon, and the obtained solution is transferred to a non-polar macroporous adsorption resin for adsorption, and then the resin column is analyzed with 75% ethanol to collect the analysis solution; the analysis solution is evaporated, concentrated and dried to obtain Avila Mycin finished product 194g, content 83.6%, yield 56.1%.

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Abstract

The invention discloses a method for extracting avilamycin from avilamycin fermentation broth. The avilamycin is prepared through the steps of performing solid-liquid separation on the avilamycin fermentation broth, performing drying, performing decoloring, and the like. The method has the advantages of high production efficiency, low cost, less environmental pollution, and good finished product quality. The avilamycin is an oligosaccharide antibiotic belonging to an orthosomycin family. The avilamycin has an inhibitory effect on various gram-positive bacteria, including some pathogenic bacteria such as vancomycin antagonistic enterococcus, methicillin antagonistic staphylococci and penicillin antagonistic diplococcus pneumoniae. Moreover, the avilamycin has an indirect effect on colibacillus, and can not only influence adhesion of bacterial flagella and bacteria but also achieve inhibition of bacterial infection by inhibiting the bacteria from adhering to surfaces of host mucosal cells, thereby inhibiting infection of diseases.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for extracting avelamycin. Background technique [0002] Avelamycin belongs to the oligosaccharide antibiotics of the orthosaccharomycin family. This product has inhibitory effect on a variety of Gram-positive bacteria, including some pathogenic bacteria, such as vancomycin-antagonistic Enterococcus, methicillin-antagonistic Staphylococcus and penicillin-antagonistic Streptococcus pneumoniae. In addition, avelamycin has an indirect effect on Escherichia coli, that is, it can affect bacterial flagella and bacterial adhesion, and inhibit bacterial infection by inhibiting bacterial adhesion to the surface of host mucosal cells, thereby inhibiting the progression of the disease. Infect. [0003] Avelamycin can be obtained from fermentation broth through separation, extraction, purification and drying. Patent CN201210544526.7 involves crystallization, silica g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/04C07H1/06
CPCC07H1/06C07H17/04
Inventor 范炜照
Owner HEBEI SHENGXUE DACHENG PHARMA
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