Micro-fluidic chip for preparation of liposome by multiple emulsion method, and manufacturing method of micro-fluidic chip
A chip and emulsion technology, applied in the field of biomedicine, can solve the problems that cannot meet the size requirements of nano-drug carriers, and achieve the effects of good economy and practicability, high encapsulation efficiency, and easy processing and preparation
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[0057] The preparation method of double emulsion preparation chip
[0058] The preparation method of double emulsion preparation chip of the present invention may comprise the following steps:
[0059] (1) Transfer the microchannel structure of the double emulsion preparation chip to the film mask plate, which is divided into a first layer mask plate and a second layer mask plate; wherein, the first layer includes a filter structure 1 and a first layer mask plate The mixing part 2; the second layer includes an outer water phase pipeline 3, an intermediate phase pipeline 4, an inner water phase pipeline 5, a W / O emulsion forming cavity 6, an outer water phase sampling part 7, an intermediate phase sampling part 8, an inner Water phase sampling part 9, second mixing part 10, W / O / W emulsion forming channel 11, double emulsion collecting part 12;
[0060] (2) Carry out the first layer of photolithography, control the height of the first layer, and obtain the template after the fi...
Embodiment 1
[0088] Embodiment 1 double emulsion preparation chip and its obtaining method
[0089] 1-1: The chip prepared by double emulsion of the present invention, the structural diagram can be found in figure 1 .
[0090] 1-2: The preparation method of the double emulsion preparation chip may include the following steps:
[0091] (1) Transfer the microchannel structure of the microfluidic chip to a film mask, which is divided into a first layer mask and a second layer mask; wherein, the first layer includes a filter structure 1 and a first layer The mixing part 2; the second layer includes an outer water phase pipeline 3, an intermediate phase pipeline 4, an inner water phase pipeline 5, a W / O emulsion forming cavity 6, an outer water phase sampling part 7, an intermediate phase sampling part 8, an inner Water phase sampling part 9, second mixing part 10, W / O / W emulsion forming channel 11, double emulsion collecting part 12;
[0092] (2) The first layer of photolithography:
[009...
Embodiment 2
[0123] The preparation of embodiment 2 liposomes
[0124] 2-1: Prepare double emulsion chips with reference to the method in Example 1. The height of each channel structure in the first layer is 5 microns. Therefore, the height of the first mixing part 2 is 5 microns, and the height of the first mixing part 2 is 5 microns. The width is 40 microns. The height of each channel in the second layer is 50 microns, therefore, the height of the W / O emulsion forming channel 6 is 50 microns.
[0125] 2-2: Configuration of three-phase solution:
[0126] (1) Inner water phase: 2mM calcein, 50mg / ml poloxamer (molecular weight 8350) dissolved in PBS buffer;
[0127] (2) Intermediate phase: Dissolve EPC and cholesterol in chloroform at a molar ratio of 2:1, blow dry with nitrogen, and dry at room temperature for at least 2 hours; dissolve phospholipids in ethanol to prepare a 50mg / ml stock solution, and place it at -20°C For storage, when experiments are required, dissolve the stock solut...
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Abstract
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