Preparation method of L-arginine-alpha-ketoglutaric acid

Abstract

The invention provides a preparation method of L-arginine-alpha-ketoglutaric acid. A method for separating a mixed solution of an alpha-ketoglutaric acid enzyme conversion solution and an arginine fermentation solution to extract arginine-alpha-ketoglutaric acid is utilized; according to the process, the enzyme conversion solution is firstly obtained by utilizing an enzyme catalysis method; after thalli in the conversion solution are removed, the conversion solution passes through a cation column to collect an alpha-ketoglutaric acid solution for later use; then, the arginine fermentation solution is obtained through a direct fermentation method and the thalli, almost protein and pigments in the fermentation solution are removed through membrane filtering; a membrane filtering solution passes through the cation column and arginine is adsorbed; then the solution is eluted with ammonia water and an arginine high fraction is collected; after deamination is carried out, an arginine solution is obtained; the two solutions are mixed according to the equal mol ratio of an alpha-ketoglutaric acid enzyme and the arginine; then operation units of de-colording by active carbon, decompressing and concentrating in vacuum, spraying and drying a finished product and the like are used for obtaining an arginine-alpha-ketoglutaric acid crystal with the purity of 98.0 percent and the yield reaches 82 percent or more.

Description

technical field [0001] The invention relates to the technical field of producing organic acids by fermentation, in particular to a method for preparing L-arginine-α-ketoglutarate. Background technique [0002] L-arginine-α-ketoglutarate (AAKG), referred to as spermone mixture, is composed of L-L-arginine (L-Arg) and α-ketoglutarate ( α-KG) The amino acid salt formed by complexation can crystallize out from the solution in the form of salt. Under different crystallization conditions, AAKG can form two forms: monoarginine ketoglutarate (MAAKG) and double arginine ketoglutarate (DAAKG). Its crystal form is colorless prism, easily soluble in water, slightly soluble in ethanol, insoluble in acetone, ether, etc., and can be completely dissociated into L-arginine and α-ketoglutarate in aqueous solution. The AAKG studied in this paper refers to MAAKG, and its structural formula is as follows: [0003] [0004] AAKG not only has the physiological functions of L-arginine and α-k...

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Problems solved by technology

[0009] In Chinese patent CN 102531968A, the source of α-ketoglutaric acid in the process of extracting sperm-ketone mixture is obtained through the reaction of 2,2-dichloroglutaric acid dimethyl ester. The synthesis route is relatively long and a large amount of organic solvents are used. , is highly toxic to the human body, and is not suitable for use as raw materials for food and medicinal products; the α-ketoglutaric acid involved in the extraction process in the Chinese patent CN 102020593A is obtained through microbial fermentation, and the by-products of the fermentation liquid have complex components (especially the accumulation of organic acids in the metabolic process), causing certain interference to the later extraction

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