Microbiological product capable of flocculation and coagulation enhancement and preparation method, use method and application thereof
A technology of microorganisms and products, which is applied in the field of microbial flocculation-aided products and their preparation, can solve the problems of difficult mass production, long time required, and increased industrial production costs, etc., and achieve the effect of wide application and good activity
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Embodiment 1
[0033] This embodiment provides a microbial flocculation-assisted coagulation product, which is a biomacromolecule produced by marine flocculation-assisted microorganisms, which has flocculation and coagulation-assisted effects, and exists in the form of pharmaceuticals or powders, and has many characteristic positions (λex / λem), including one or several of 230 / 290 nm, 260 / 445 nm, 275 / 325 nm, and 355 / 430 nm; the biomacromolecules are humic acid and fulvic acid , phenolic amino acids, glycoproteins, mucopolysaccharides, proteins, cellulose, one or more of deoxyribonucleic acid.
[0034] For the microbial flocculation-assisted coagulation product of the present invention, the present invention designs the preparation method of the microbial flocculation-assisted coagulation product:
[0035] figure 1 It is a flow chart of the preparation method of the microbial flocculation aid product in the embodiment of the present invention, as figure 1 Shown, the preparation method of mic...
Embodiment 2
[0042] This embodiment provides a preparation method of microbial flocculation aid product, comprising the following steps:
[0043] S1: Weigh 1000 grams of seawater, 500 milligrams of peptone, 500 milligrams of phenol, 150 milligrams of dipotassium hydrogen phosphate, 50 milligrams of magnesium sulfate, 25 milligrams of calcium chloride and 20 milligrams of ferrous sulfate, stir and mix to obtain seawater medium, and then add to the Add 10 grams of sea mud to the seawater culture medium, and cultivate it in an environment with a temperature of 30 degrees Celsius, a dissolved oxygen amount of 2.5 mg / L, and a pH value of 7.5 until obvious colonies appear, and then absorb the bacterial liquid for preservation. It has been identified that the microorganism belongs to Arthrobacter , and is Gram-positive bacteria;
[0044] S2: Add the aspirated Arthrobacter marine bacteria liquid into 1 liter of seawater culture medium, carry out enrichment culture for 2 days under the environment ...
Embodiment 3
[0048] This embodiment provides a preparation method of microbial flocculation aid product, comprising the following steps:
[0049] S1: Weigh 1000 grams of seawater, 1000 milligrams of hydroquinone, 400 milligrams of peptone, 120 milligrams of dipotassium hydrogen phosphate, 40 milligrams of magnesium sulfate, 20 milligrams of calcium chloride and 15 milligrams of ferrous sulfate, stir and mix to obtain seawater medium, Then add 25 grams of sea mud to the seawater culture medium, and cultivate it at a temperature of 25 degrees Celsius, a dissolved oxygen amount of 1.5 mg / liter, and a pH value of 7 until obvious colonies appear, and then absorb the bacterial liquid for preservation. After identification, the microorganism Belongs to marine proteobacteria and is Gram-negative bacteria;
[0050] S2: Add the aspirated bacterial solution into 1 liter of seawater culture medium, carry out enrichment culture for 3 days in an environment with a temperature of 25 degrees Celsius, a di...
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