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Method for analyzing otis tarda food source plant through feces DNA (Deoxyribonucleic Acid)

A technology of great bustards and plants, which is applied to the analysis of food source plants of great bustards by fecal DNA and the analysis of food habits of great bustards, can solve the problems of unclear food types and nutritional requirements of great bustards, lack of scientific guidance, and unsuccessful artificial breeding.

Active Publication Date: 2017-02-08
RES INST OF FORESTRY NEW TECH CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the food types and nutritional requirements of the bustard in each life history period are still unclear, resulting in the lack of scientific guidance for the conservation department to protect wild bustard populations and foraging grounds, which is also the bottleneck of the unsuccessful artificial breeding of the bustard for many years

Method used

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  • Method for analyzing otis tarda food source plant through feces DNA (Deoxyribonucleic Acid)
  • Method for analyzing otis tarda food source plant through feces DNA (Deoxyribonucleic Acid)
  • Method for analyzing otis tarda food source plant through feces DNA (Deoxyribonucleic Acid)

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Experimental program
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Effect test

Embodiment 1

[0115] Example 1. Analysis of Great Bustard Food Source Plants Based on Chloroplast DNA trnL Barcodes

[0116] (1) Establishment of the trnL complete sequence database of native plant chloroplast DNA

[0117] 1. Collection of potential food plants

[0118] Collect plant leaves at the site of great bustard activity or where there are traces of great bustard activity (feces, feathers, scratches, etc.), put the fresh leaves of the plant into a paper bag, add silica gel, and store in a dry place. Record the family, genus, and species information of the collected plants.

[0119] 2. Plant DNA extraction

[0120] The leaves were put into a mortar, ground into powder with liquid nitrogen, and the plant genomic DNA was extracted using a broad-spectrum plant genomic DNA rapid extraction kit (article number: DL116-01, Beijing Biomed Gene Technology Co., Ltd.).

[0121] Perform agarose electrophoresis on the extracted DNA, and use a UV spectrophotometer to measure the concentration an...

Embodiment 2

[0174] Example 2, method verification based on feces DNA analysis of bustard sex

[0175] (1) Collect known plants to feed the Great Bustard in cages and collect feces

[0176]In places where there are bustard activities or traces of bustard activities in the wild (feces, feathers, scratches, etc.), 10 whole plants of 8 kinds of plants were collected, and the plant species were Mongolian allium (Androsace umbellata) and Diandimei (Anemarrhenaasphodeloides), Bupleurum chinense, Leymus chinensis, Chloris virgata, Rhaponticum uniflorum, Scabiosa comosa, Potentilla discolor. In addition, 2 plant staples, mung beans (Vigna radiata) and maize (Zea mays), were fed.

[0177] Before feeding the caged bustard, remove old feces, dropped food and weeds on the ground in advance to avoid interfering with subsequent analysis. Select 6 adult great bustards with good daily eating conditions as the experimental subjects, and give them enough and clean water during the feeding period. No food...

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Abstract

The invention discloses a method for analyzing an otis tarda food source plant through feces DNA (Deoxyribonucleic Acid). The method comprises the following steps of (1) using trnL gene amplification primer pairs with different base tags for respectively amplifying multiple trnL gene segments in otis tarda feces to be measured, so as to obtain the multiple trnL gene segments in the otis tarda feces to be measured added with different base tag sequences; (2) mixing the multiple trnL gene segments in the otis tarda feces to be measured added with the different base tag sequences, so as to obtain mixed segments, high-throughput sequencing the mixed segments, so as to obtain multiple sequencing sequences of the trnL gene segments in the otis tarda feces to be measured; (3) comparing the multiple sequencing sequences of the trnL gene segments in the otistarda feces to be measured with an eciophyte chloroplast DNA trnL complete sequence database and a common trnL bar code database, judging the otis tarda food source plant to be measured according to the homology of sequences in the sequencing sequences and the two databases, and calculating the percentage of a plant ingested by an otis tarda according to a sequence bar number. The method for analyzing the otis tarda food source plant through feces DNA provided by the invention provides a technical support for researching feeding habit preference of animals and recipes.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for analyzing the feeding properties of the Great Bustard, in particular to a method for analyzing the source plants of the Great Bustard through feces DNA. Background technique [0002] Diet analysis is one of the important topics in the study of wildlife conservation biology. It is also the basis for understanding the relationship between animals and habitats, and can provide scientific support for the effective protection and management of wild animals. Traditional methods for analyzing animal feeding habits include field observation of foraging behavior, analysis of stomach contents, and microscopic identification of feces components. However, these methods have the disadvantages of invasiveness, poor operability, and low accuracy. For example, field observation is poorly operable and the results are extensive, and the analysis of stomach contents can cause damage or eve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895
Inventor 刘刚龚明昊崔丽娟李林海宁宇韩·莫日根周景英于长江李惠鑫夏晓飞
Owner RES INST OF FORESTRY NEW TECH CHINESE ACAD OF FORESTRY
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