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Microbe-assisted synthesis and degradable oil-water separation omentum and preparation method thereof

A technology of oil-water separation omentum and microorganisms, which is applied in the field of oil-water separation omentum and its preparation, can solve the problems of restricting large-scale practical application, poor water resistance of omentum, and affecting service life, so as to improve the oil-water separation effect and separation performance Stable, low-cost effect

Inactive Publication Date: 2018-08-21
优澎(嘉兴)新材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese patent application with the application number 201010531517.5 discloses an oil-water separation net with underwater super-oleophobic properties. This technology has a certain effect of oil-water separation, but the preparation requires light-induced polymerization, and the process is relatively complicated.
The Chinese patent application whose publication number is CN102716676A discloses a method for preparing an oil-water separation net with underwater superoleophobic properties using chitosan as a raw material. The film-forming material used in this patent is a polymer with certain water solubility, resulting in The omentum has poor water resistance, which affects its service life
The Chinese patent application with application number 201210152727.2 reports a naturally degradable underwater superoleophobic oil-water separation membrane, but its preparation is also slightly complicated, which limits its large-scale practical application

Method used

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  • Microbe-assisted synthesis and degradable oil-water separation omentum and preparation method thereof

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preparation example Construction

[0020] The preparation method of the degradable oil-water separation omentum that is assisted by microorganisms to be synthesized by the present invention comprises the following steps:

[0021] (1) Microbial liquid seed medium and microbial solid-state fermentation medium are prepared respectively:

[0022] Preparation of microbial liquid seed medium:

[0023] Add 2% glucose, 0.5% peptone, 0.5% yeast powder, 0.27% disodium hydrogen phosphate pentahydrate and 0.115% citric acid-hydrate with a mass concentration of less than 3 g / L in deionized water according to the following mass percentages. Sulfuric acid with a percentage concentration of 0.2% adjusts the pH value to 4-6, sterilizes at 115 degrees Celsius for 30 minutes, and shakes fully to obtain a microbial liquid seed medium;

[0024] Preparation of microbial solid-state fermentation medium:

[0025] Add 2% glucose, 0.5% peptone, 0.5% yeast powder, 0.27% disodium hydrogen phosphate pentahydrate, 0.2% agar and 0.115 citr...

Embodiment 1

[0033] (1) Preparation of microbial fermentation medium:

[0034] Preparation of microbial liquid seed medium:

[0035] Add 2% glucose, 0.5% peptone, 0.5% yeast powder, 0.27% disodium hydrogen phosphate pentahydrate and 0.115% citric acid-hydrate with a mass concentration of less than 3 g / L to 250 mL of deionized water in the following mass percentages to Sulfuric acid with a mass concentration of 0.2% adjusts the pH value to 5, sterilizes at 115 degrees centigrade for 30 minutes, and vibrates fully to obtain a microbial liquid seed medium for use.

[0036] Preparation of microbial solid-state fermentation medium:

[0037]Add 2% glucose, 0.5% peptone, 0.5% yeast powder, 0.27% disodium hydrogen phosphate pentahydrate, 0.2% agar and citric acid-water with a mass concentration of less than 3 g / L to 60 mL of deionized water according to the following mass percentages: 0.115% of the compound was adjusted to pH 5 with sulfuric acid with a mass concentration of 0.2%, sterilized at ...

Embodiment 2

[0044] (1) Prepare liquid seed medium and solid-state fermentation medium of microbial strains according to the method in Example 1.

[0045] (2) Inoculate the commercial Acetobacter xylinum strain into the seed culture medium with an incorporation amount of 2%, and vibrate at 30 degrees Celsius for 24 hours to obtain a seed culture solution.

[0046] (3) Cut the sterilized cotton gauze into a 4cm × 6cm size sample, spread it on the surface of the microbial solid-state fermentation medium prepared in the above step (1), wherein the yarn count in the cotton gauze is 30 The yarn count density is 50×60.

[0047] (4) the seed culture liquid of the Acetobacter xylinum bacterial classification that step (2) gained is added dropwise on the cotton gauze in step (3), until gauze is soaked completely, at 30 degrees Celsius, static culture 24 hours.

[0048] (5) Place the composite membrane obtained in step (4) in 6% sodium hydroxide aqueous solution at 80 degrees Celsius for 40 minutes...

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Abstract

The invention relates to a degradable oil-water separation mesh membrane synthesized through assistance of microorganisms and a preparation method of the degradable oil-water separation mesh membrane, and belongs to the technical field of oil-water separation in biochemical engineering and oil industry. The oil-water separation mesh membrane is prepared by compositing a cotton gauze with hydrophilic nanofibers prepared from the microorganisms or secretions thereof. The degradable high-efficiency oil-water separation mesh membrane with the underwater superoleophobic property is formed by winding the fiber-shaped microorganisms or secretions thereof on yarn of the cotton gauze in a wrapping mode. The separation mesh membrane can be used for oil-water two-phase separation in the fields of biology, chemical industry, environmental protection and the like, the pore permeability and the oil-water separation effect are effectively improved, and the separation performance is stable; in addition, the preparation process is simple, the raw materials have rich sources and are easy to obtain, and the separation mesh membrane does not contain harmful substances and can be degraded, so that the separation mesh membrane is green and friendly to environment.

Description

technical field [0001] The invention relates to a degradable oil-water separation mesh synthesized with the assistance of microorganisms and a preparation method thereof, belonging to the technical field of oil-water separation in biochemical industry and petroleum industry. Background technique [0002] Crude oil spills caused by oil extraction and its pipeline transportation and ship transportation, as well as large amounts of oily wastewater discharged in food processing, machinery industry and chemical industry will have a very harmful impact on human beings and the natural environment. In addition, with the depletion of fossil resources, people pay more and more attention to the separation and recovery of waste oil from the oil-water mixed system. With the development of the biochemical industry, it is becoming more and more important to separate the biosynthetic chemicals dissolved in the oil phase from the oil-water mixed system in a more economical and effective way....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D17/022C02F1/40
CPCB01D17/02C02F1/40
Inventor 赛华征付蕊向军辉张复实
Owner 优澎(嘉兴)新材料科技有限公司
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