Method for purifying high-quality acarbose

A technology of acarbose and a purification method, which is applied in the field of pharmaceutical purification, can solve the problems of large amount of elution solvent, low yield of acarbose, and poor desalination effect, so as to save organic solvents and energy consumption, save The effect of eliminating the column packing step and shortening the production cycle

Active Publication Date: 2017-02-15
HANGZHOU ZHONGMEI HUADONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the problems of poor desalination effect of the existing process, which leads to the need for multiple columns for subsequent purification, too long production cycle, low yield of acarbose, large amount of elution solvent, polluting the environment, and unfavorable for large-scale industrial production.

Method used

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  • Method for purifying high-quality acarbose
  • Method for purifying high-quality acarbose
  • Method for purifying high-quality acarbose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 10 tons of the pretreated acarbose fermentation liquid, the concentration is 0.6% (W / W), and the pH value is adjusted to 4.8 with filter aid aluminum sulfate.

[0047] The elution temperature of the anion and cation exchange resin column chromatography in this embodiment is 20°C.

[0048] Add strong acidic cation exchange resin chromatography column (filler: CT151, chromatography column inner diameter: 1000mm; filling height: 2500mm), pass through the cation exchange resin chromatography column at a volume of 1 times per hour, and collect 12000 liters of feed liquid .

[0049] Lower the liquid level to about 10cm above the resin surface, then pass through 1.5 times the volume of 0.03mol / L hydrochloric acid to top wash the resin at a rate of 1 times the volume per hour, and collect 3000 liters of eluent.

[0050] The mixture of the above feed liquid and eluent is determined by ion chromatography for Ca 2+ 、Na + The total concentration of cations was 17mg / L.

[0051] ...

Embodiment 2

[0061] 10 tons of the pretreated acarbose fermentation liquid, the concentration is 0.5% (W / W), and the pH value is adjusted to 4.5 with filter aid aluminum sulfate.

[0062] The elution temperature of the anion and cation exchange resin column chromatography in this embodiment is 25°C.

[0063] Add strong acidic cation exchange resin chromatography column (filler: HZ-12, chromatography column inner diameter: 1000mm; filling height: 2000mm), pass through the cation exchange resin chromatography column at a volume of 0.5 times per hour, and collect the feed liquid 9500 liters.

[0064] Lower the liquid level to about 10cm above the resin surface, then feed 1 volume of 0.04mol / L hydrochloric acid to top wash the resin at a rate of 1.5 volumes per hour, and collect 2000 liters of eluent.

[0065] The mixture of the above feed liquid and eluent is determined by ion chromatography for Ca 2+ 、Na + The total concentration of cations is 25mg / L.

[0066] Pass 9500 liters of feed li...

Embodiment 3

[0070] 10 tons of the pretreated acarbose fermentation liquid, the concentration is 0.7% (W / W), and the pH value is adjusted to 4.9 with filter aid aluminum sulfate.

[0071] The elution temperature of the anion and cation exchange resin column chromatography in this embodiment is 15°C.

[0072] Add strong acidic cation exchange resin chromatography column (filler: JK006, column inner diameter: 1000mm; filling height: 3000mm), pass through the cation exchange resin chromatography column at 1.5 times the volume per hour, and collect 9400 liters of feed liquid .

[0073] Lower the liquid level to about 10cm above the resin surface, then pass through 2 times the volume of 0.02mol / L hydrochloric acid to top wash the resin at a rate of 0.5 times the volume per hour, and collect 4000 liters of eluent.

[0074] The mixture of the above feed liquid and eluent is determined by ion chromatography for Ca 2+ 、Na + The total concentration of cations was 27mg / L.

[0075] Pass 9400 liter...

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Abstract

The invention relates to the technical field of medicine purification, and in particular discloses a method for purifying high-quality acarbose. The method comprises the following steps: removing cation salt of a pretreated fermented acarbose liquid by using a cation resin column, performing top washing by using a diluted hydrochloric acid solution so as to elute a small amount of acarbose adsorbed in the resin, and removing anions by using a common method. By adopting the method, the problems that a conventional process is poor in salt removing effect so that the pretreated fermented acarbose liquid needs to be purified with the cation resin column for multiple times, too long in production cycle, low in acarbose yield, large in use amount of an elution solvent, severe in environment pollution and unbeneficial to industrial large-scale production can be solved. The acarbose prepared by using the method is high in purity, stable quality and medicine use safety of the acarbose can be ensured, moreover a great deal of production cost of industrial large-scale production can be reduced, outstanding economic benefits can be made, and sustainable development of companies can be facilitated.

Description

technical field [0001] The invention relates to the technical field of medicine purification, in particular to a purification method for industrial production of high-quality acarbose. Background technique [0002] Acarbose (Acarbose) is a new oral hypoglycemic drug. Competitively inhibits glucoside hydrolases in the gut. Reduce the decomposition of polysaccharides and sucrose into glucose, so that the absorption of sugar is slowed down accordingly, so oral acarbose can control the rise of postprandial blood sugar in patients with type II diabetes. [0003] Chinese invention patent CN86108259 discloses a method for preparing high-purity acarbose, that is, the acarbose aqueous solution that is about to be pretreated is passed through a weakly acidic hydrophilic cation exchanger (with dextran, agarose and cellulose or is made of Adding the exchanger derived from the above components of polyacrylamide) to purify the acarbose. But this method needs to strictly control the pH ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/06
CPCC07H1/06C07H15/203
Inventor 许乐义徐亚强谢海松
Owner HANGZHOU ZHONGMEI HUADONG PHARMA
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