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Method for extracting superoxide dismutase from pig blood and the superoxide dismutase

A superoxide and dismutase technology, applied in the direction of oxidoreductase, biochemical equipment and methods, enzymes, etc., can solve the problems of harmful chemical reagents, organic solvents, secondary pollution, etc., to avoid environmental pollution and enzyme activity The effects of toxicity, short production cycle and high activity

Inactive Publication Date: 2020-02-18
HUBEI BAODI AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The first object of the present invention is to provide a method for extracting superoxide dismutase from pig blood, to solve the problem of using harmful chemical reagents and organic Solvent, the problem of secondary pollution, the method for extracting superoxide dismutase from pig blood, the process uses rich pig blood as raw material, adjusts pH through inorganic acid and alkali and concentrates by ultrafiltration, with simple process , low cost, green environmental protection, easy to implement and other advantages

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  • Method for extracting superoxide dismutase from pig blood and the superoxide dismutase

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Experimental program
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Effect test

Embodiment 1

[0050] (1) Collect 1000L of fresh pig blood that has passed inspection, add 0.5% of its mass of food-grade sodium citrate to the fresh pig blood for anticoagulation treatment, use a tubular centrifuge at 16300r / mim, and collect the blood after continuous separation and centrifugation Red blood cells, 420L of blood cell fluid and 580L of plasma fluid are obtained. Add water equal in volume to the blood cell fluid and stir for 60 minutes to obtain 840L of hemolysis;

[0051] (2) Adjust the pH of the hemolysis in step (1) to 5.2 with HCl, quickly raise the temperature to 60°C and keep it warm for 50 minutes, then quickly drop the temperature to below 45°C, and filter by a plate and frame filter press to obtain the filtrate 560L;

[0052] (3) Adjust the pH value of the filtrate in step (2) with NaOH to 6.2, quickly raise the temperature to 70°C and keep it warm for 20 minutes, then quickly lower the temperature to below 40°C, and filter through a plate and frame filter press to obtain ...

Embodiment 2

[0056] (1) Collect 1000L of fresh pig blood that has passed inspection, add 0.6% food-grade sodium citrate to the fresh pig blood for anticoagulation treatment, use a tubular centrifuge at 16300r / mim, and collect the blood after continuous separation and centrifugation Red blood cells, that is, 450L of blood cell fluid and 550L of plasma fluid are obtained. Add water equal in volume to the blood cell fluid and stir for 40 minutes to obtain 900L of hemolysis;

[0057] (2) Adjust the pH of the hemolysis in step (1) to 5.5 with HCl, quickly raise the temperature to 60°C and keep it warm for 40 minutes, then quickly drop the temperature to below 45°C, and filter by a plate and frame filter press to obtain the filtrate 610L;

[0058] (3) Use NaOH to adjust the pH value of the filtrate in step (2) to 6.3, quickly raise the temperature to 70°C and keep it for 25 minutes, then quickly drop the temperature to below 45°C, filter by a plate and frame filter press to obtain 520L of filtrate ;...

Embodiment 3

[0062] (1) Collect 2000L of fresh pig blood that has passed inspection, add 1.0% of its mass of food-grade sodium citrate to the fresh pig blood for anticoagulation treatment, use a tubular centrifuge at 16300r / mim, and collect the blood after continuous separation and centrifugation Red blood cells, that is, 930L of blood cell fluid and 1070L of plasma fluid are obtained. Add the same volume of water as the blood cell fluid and stir for 60 minutes to obtain 1860L of hemolysis;

[0063] (2) Adjust the pH of the hemolyzed blood in step (1) to 5.3 with phosphoric acid, quickly raise the temperature to 65°C and keep it warm for 50 minutes, then quickly drop the temperature to below 45°C, and filter through a plate and frame filter press to obtain the filtrate 1230L;

[0064] (3) Adjust the pH value of the filtrate in step (2) with KOH to 6.5, quickly raise the temperature to 75°C and keep it for 30 minutes, then quickly lower the temperature to below 45°C, filter by a plate and frame ...

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Abstract

The invention provides a method for extracting superoxide dismutase from pig blood and the superoxide dismutase. The method has the advantages that the pig blood is used as the raw material, no organic solvents are used in the extraction and separation process of the superoxide dismutase, environment pollution and damage on enzyme activity are avoided, and the production process is simplified; lauroyl chloride is used as the protecting agent during the purification of the superoxide dismutase, the superoxide dismutase is protected from being destroyed by heat, acid and alkali, the thermal denaturation technology and the isoelectric precipitation technology are combined to further purify and refine crude superoxide dismutase, and the method is short in production cycle, low in cost and suitable for large-scale industrial production; the prepared superoxide dismutase is high in activity, and the unit enzyme activity of the superoxide dismutase reaches more than 4500U / mg dry powder.

Description

Technical field [0001] The invention relates to the field of separation and purification of organic matter, in particular to a method for extracting superoxide dismutase from pig blood and the superoxide dismutase. Background technique [0002] Superoxide Dismutase (Superoxide Dismutase, referred to as SOD) is a vital antioxidant enzyme that exists in organisms. It is an internationally recognized superoxide anion oxygen free radical (O 2- ) Is a special scavenger, which plays a vital role in balancing the body's oxidation and antioxidant systems and avoiding free radical damage. The effect of SOD on the human body can be classified as "one clear and four resistances": "one clear" means to effectively remove superoxide anion oxygen free radicals in the body and protect human cells from the poison of oxygen free radicals; "four resistances" means anti-aging , Anti-disease, anti-radiation and anti-fatigue. [0003] In recent years, the technology for preparing superoxide dismutase (...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02
CPCC12N9/0089C12Y115/01001
Inventor 陈石良
Owner HUBEI BAODI AGRI TECH