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Activated fluorescent latex microsphere for 25-hydroxyvitamin D3 fluorescent immunochromatography

A technology of fluorescent latex microspheres and fluorescent immunochromatography, which is applied in the direction of analytical materials, measuring devices, instruments, etc., can solve the problems of reduced detection sensitivity, uneven dispersion, chromatographic failure, etc., and achieve stable and accurate detection results , the effect of easy operation

Active Publication Date: 2017-02-15
BEIJING ELCOTEQ BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: after drying, the fluorescent latex microspheres of the fluorescent immunochromatography detection card are easy to agglomerate and disperse unevenly, resulting in failure of chromatography or reduction of detection sensitivity

Method used

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  • Activated fluorescent latex microsphere for 25-hydroxyvitamin D3 fluorescent immunochromatography
  • Activated fluorescent latex microsphere for 25-hydroxyvitamin D3 fluorescent immunochromatography
  • Activated fluorescent latex microsphere for 25-hydroxyvitamin D3 fluorescent immunochromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Polyethylene glycol monolaurate, sodium dodecylbenzenesulfonate and lauroyl glutamate

[0047] 1) Dissolve the surfactant (5mg polyethylene glycol monolaurate, 1mg sodium dodecylbenzenesulfonate and 4mg sodium lauroyl glutamate) in 0.5M carbonic acid buffer (H value to 9.6), then add in 0.1ml of DMF, 0.1ml of DMF, after adding 1mg of N,N'-dicyclohexylcarbodiimide (DCC) and 0.6mg of N-hydroxysuccinimide (NHS) Stir at room temperature for 3 hours.

[0048] 2) Adjust the pH value of 1ml of fluorescent latex microsphere solution containing 1% solids on the amino surface to 9.6 with 0.5M carbonic acid buffer solution of pH 9.6, then add it to step 1), and continue to stir and react for 3 hours. Acquired activated latex microspheres.

[0049] 3) Use a high-speed centrifuge to centrifuge the fluorescent latex microsphere reaction solution at high speed, remove the supernatant of the reaction solution, and redissolve the fluorescent latex microspheres with 1mL microsphere buf...

Embodiment 2

[0052] The operation method of this example is similar to that of Example 1, except that the surfactant in step 1) is: 2 mg polyethylene glycol monolaurate, 2 mg sodium dodecylbenzene sulfonate and 6 mg lauroyl glutamate Sodium Amino Acid.

Embodiment 3

[0054] The operation method of this example is similar to Example 1, except that the surfactant in step 1) is: 6.5mg polyethylene glycol monolaurate, 0.5mg sodium dodecylbenzenesulfonate and 3mg lauryl Sodium acyl glutamate.

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Abstract

The invention discloses an activated fluorescent latex microsphere for a 25-hydroxyvitamin D3 fluorescent immunochromatographic detection card and the detection card. The preparation method of the activated fluorescent latex microsphere comprises the following steps: 1) adding a surfactant into a buffer solution with pH of 8 to 10 and adding dimethylformamide, N, N'-dicyclohexylcarbodiimide and N-hydroxysuccinimide into the solution, and 2) taking a dispersion liquid of fluorescent latex microspheres with amido groups on the surfaces, adjusting pH of the dispersion liquid to 8-10 through a buffer solution, adding the dispersion liquid into the mixture obtained in the step 1), carrying out stirring for a reaction, then carrying out centrifugation after the reaction, and removing the supernatant so that the surface-activated fluorescent latex microspheres are obtained. The surfactant contains polyethylene glycol monolaurate, dodecyl benzene sulfonate and lauroyl glutamate according to a weight ratio of 2-10: 0.5-2: 3-7.

Description

technical field [0001] The invention belongs to the technical field of fluorescence immunochromatography, and in particular relates to an activated fluorescent latex microsphere for a 25-hydroxyvitamin D3 fluorescence immunochromatography detection card and its preparation and application. Background technique [0002] Fluorescence immunochromatography detection card (reagent card) is widely used in the field of rapid detection because of its fast and convenient characteristics. The analyte antibody is labeled with fluorescent latex microspheres, and the latex microspheres have surface electron repulsion and van der Waals forces in the liquid phase to keep the latex particles in the liquid phase evenly distributed. However, after spraying on the glass cellulose film and drying, as the solution is evaporated to dryness, the surface tension pushes the latex particles infinitely closer together, resulting in the disappearance of the intermolecular repulsion, and the increase of...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/558
CPCG01N33/533G01N33/558
Inventor 林斯
Owner BEIJING ELCOTEQ BIO TECH
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